We narrowed to 9,634 results for: SUB

1. pCbeta EV (PKA catalytic subunit Cbeta)

   Plasmid

   #15311

   Depositor

   Stanley McKnight

   Article

   Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

   Insert

   PRKACB (Prkacb Mouse)
   

   Expression

   Mammalian

   Available Since

   July 6, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pUb-HisUb-Ubc9-ChE1 v4.5

   Plasmid

   #185756

   Purpose

   Bacterial expression of His-Ub, Ubc9 and ChE1 v4.5

   Depositor

   Jeffrey Bode

   Article

   Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

   Inserts

   His-Ub
   Ubc9
   ChE1 v4.5
   

   Tags

   His6

   Mutation

   K14R

   Promoter

   T7

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Human b4 nicotinic receptor subunit EM construct

   Plasmid

   #163961

   Purpose

   pEZT-BM vector with beta4 gene after CMV promoter, published EM construct

   Depositor

   Ryan Hibbs

   Article

   Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do

   Insert

   Human beta4 nicotinic acetylcholine receptor subunit EM construct
   

   Use

   Baculovirus (bacmam) production and mammalian exp…

   Tags

   StrepII tag and apocytochrome b(562)RIL (BRIL)

   Expression

   Mammalian

   Mutation

   Pro341-Ser395 replaced with apocytochrome b(562)R…

   Promoter

   CMV

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFSynW Substance P-pHluorin IRES mRuby3

   Plasmid

   #195699

   Purpose

   Lentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

   Depositor

   Edwin Chapman

   Article

   Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

   Insert

   Tac1 (Tac1 Mouse)
   

   Use

   Lentiviral

   Tags

   pHluorin

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-mCherry-PKAsub-RLuc8N-NES

   Plasmid

   #138211

   Purpose

   Encodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   mCherry-PKAsub-RLuc8N-NES
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAM420 GFP-titin-ssrA substrate

   Plasmid

   #226362

   Purpose

   Expression of GFP-titin-ssrA substrate for 26S proteasome

   Depositor

   Andreas Martin

   Article

   Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

   Insert

   GFP-titin substrate
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PCDF Bravo-PotH-OL3/2sub

   Plasmid

   #216752

   Purpose

   Study the interaction of PotH in E. coli, specifically focusing on the variant in which outer loop 2 (OL2) is substituted with OL3 in its interaction with exogenous peptides.

   Depositor

   Gordon Laurie

   Article

   Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

   Insert

   potH (potH E. coli (Bacteria))
   

   Tags

   10x His

   Expression

   Bacterial

   Mutation

   Outer loop 2 (OL2) with the sequence of [WMGILKNN…

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Anti-Gs protein, alpha subunit [N192/12]

   Plasmid

   #190302

   Purpose

   Mammalian Expression Plasmid of anti-Gs protein, alpha subunit (Cattle). Derived from hybridoma N192/12.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Gs protein, alpha subunit (Bos taurus) recombinant Mouse monoclonal antibody (GNAS Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. psubCMV-WPRE-IL3SP-mVEGF-D-dNdC

   Plasmid

   #119229

   Purpose

   Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.

   Depositor

   Kari Alitalo

   Insert

   IL3SP-Vegfd-dNdC (Vegfd Mouse)
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-mCherry-PKCsub-RLuc8N-NES

    Plasmid

    #138213

    Purpose

    Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    mCherry-PKCsub-RLuc8N-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES

    Plasmid

    #138299

    Purpose

    Encodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    mCherry-PKCsub(T/A)-RLuc8N-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX408 Francisella tularensis subsp. novicida Cas9

    Plasmid

    #68705

    Purpose

    Francisella tularensis subsp. novicida Cas9

    Depositor

    Feng Zhang

    Insert

    FtCas9
    

    Use

    CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. untargeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45215

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    untargeted Aurora B FRET sensor
    

    Tags

    CyPET, YPET

    Expression

    Mammalian

    Promoter

    t7

    Available Since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. untargeted Plk1 FRET sensor Myt1 substrate

    Plasmid

    #45217

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    untargeted Plk1 FRET sensor Myt1 substrate
    

    Expression

    Mammalian

    Promoter

    t7

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. RNA polymerase beta prime subunit FLAG

    Plasmid

    #102337

    Purpose

    Targeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.

    Depositor

    Erin O'Shea

    Article

    Piechura et al Elife. 2017 Dec 14;6. pii: 32032. doi: 1

    Insert

    RNAP Beta Prime Subunit with C-terminal FLAG TAG
    

    Tags

    3x FLAG with 3X GSGS linker

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet

    Plasmid

    #83286

    Purpose

    untargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pair

    Depositor

    Daniel Needleman

    Article

    Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

    Insert

    mTurquoise2-FHA2-AURKB substrate-YPet
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-FLEX-mCherry-FHA1-subT391A-mCherry

    Plasmid

    #104462

    Purpose

    Cre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherry

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Neuron. 2017 Dec 6;96(5):1070-1083.e5. d

    Insert

    mCherry-subT391A-FHA1-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Promoter

    CAG

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

    Plasmid

    #213118

    Purpose

    Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

    Depositor

    Scott Coyle

    Article

    Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

    Insert

    PKAsub-mCherry-MinD (minD Escherichia coli)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CENP-B targeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45214

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    CENP-B targeted Aurora B FRET sensor
    

    Expression

    Mammalian

    Promoter

    t7

    Available Since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. N-term Hec1 Plk1 FRET sensor c-jun substrate

    Plasmid

    #45204

    Depositor

    Michael Lampson

    Article

    Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

    Insert

    Hec1-targeted Plk1 FRET sensor
    

    Tags

    CyPET, YPET

    Expression

    Mammalian

    Available Since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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