We narrowed to 687 results for: emd

1. pET14b_tev_PDZ2

   Plasmid

   #59734

   Purpose

   expresses PDZ2 domain of human ZO-1 in bacteria

   Depositor

   Alan Fanning

   Article

   Fanning et al J Biol Chem. 2007 Dec 28;282(52):37710-6

   Insert

   zonula occludens-1 PDZ2 (TJP1 Human)
   

   Use

   Tags

   HIS and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pET28a-ClfB_N2N3-HIS-ybbr

   Plasmid

   #101717

   Purpose

   ClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   ClfB_N2N3
   

   Use

   Tags

   H3V 3C, HIS, and ybbr

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDJSVT89

   Plasmid

   #109303

   Purpose

   Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

   Depositor

   Daniel Slade

   Article

   Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

   Type

   Empty backbone

   Use

   Tags

   TamA 1-27, 6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDJSVT90

   Plasmid

   #109304

   Purpose

   Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

   Depositor

   Daniel Slade

   Article

   Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

   Type

   Empty backbone

   Use

   Tags

   TamA 1-27, 6His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTH849-gst-optec

   Plasmid

   #89590

   Purpose

   Plasmid for bacterial expression of His-tagged GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase codon-optimised for bacterial expression
   

   Use

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. T7-PseB-His6

   Plasmid

   #89723

   Purpose

   Expresses PseB from C. jejuni with an N-terminal T7 tag and a C-terminal His6 tag

   Depositor

   Barbara Imperiali

   Article

   Zamora et al Methods Enzymol. 2017;597:145-186. doi:

   Insert

   PseB
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28b-Sad1

   Plasmid

   #90297

   Purpose

   protein expression

   Depositor

   Christine Guthrie

   Article

   Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

   Insert

   SAD1
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28 mSmo-Cterm

   Plasmid

   #58703

   Purpose

   used for anti-mouse Smoothened antibody production (for affinity purification)

   Depositor

   Matthew Scott

   Article

   Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

   Insert

   Smo (Smo Mouse)
   

   Use

   Tags

   6X-HIS

   Expression

   Bacterial

   Mutation

   contains C terminus (aa 543-793)

   Promoter

   Available since

   Sept. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pET28b-sad1-C63A

   Plasmid

   #90302

   Purpose

   protein expression

   Depositor

   Christine Guthrie

   Article

   Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

   Insert

   SAD1
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   C63A

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET15b/GgVcl 884-1066 (aka Vt)

    Plasmid

    #46176

    Depositor

    Susan Craig

    Article

    Steimle et al J Biol Chem. 1999 Jun 25;274(26):18414-2

    Insert

    Vcl 884-1066 (VCL Chicken)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 884-1066 (tail domain)

    Promoter

    T7

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a(+)/FbGH30

    Plasmid

    #86463

    Purpose

    Expression of the Beta-1,6-Exo-Laminarinase FbGH30 in E.coli

    Depositor

    Jan-Hendrik Hehemann

    Article

    Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

    Insert

    FbGH30
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a(+)/FaGH17A

    Plasmid

    #86462

    Purpose

    Expression of the Beta-1,3-Endo-Laminarinase FaGH17A in E.coli

    Depositor

    Jan-Hendrik Hehemann

    Article

    Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

    Insert

    FaGH17A
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET27b+R

    Plasmid

    #188245

    Purpose

    Empty backbone vector for protein expression in Escherichia coli and his-tag purification

    Depositor

    Yiping Qi

    Article

    Fang et al Methods Mol Biol. 2023;2653:153-171. doi

    Type

    Empty backbone

    Use

    Tags

    6xHis tag and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTorD-H6

    Plasmid

    #169039

    Purpose

    Escherichia coli TorD with C-terminal 6xHis in pET28a.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    TorD-H6
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    The mutation N at position 46 was changed back to…

    Promoter

    T7

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28b-sad1-H79A

    Plasmid

    #90303

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

    Insert

    SAD1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    H79A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28b-sad1-H85A

    Plasmid

    #90304

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

    Insert

    SAD1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    H85A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28b-sad1-D159A

    Plasmid

    #90305

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

    Insert

    SAD1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D159A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28b-sad1-H405A

    Plasmid

    #90306

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

    Insert

    SAD1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    H405A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28b-sad1-D422A

    Plasmid

    #90307

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

    Insert

    SAD1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D422A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTAT-CRE

    Plasmid

    #35619

    Depositor

    Steven Dowdy

    Article

    Wadia et al Nat Med. 2004 Mar;10(3):310-5. Epub 2004

    Insert

    CRE (cre Bacteriophage P1)
    

    Use

    Tags

    6xHIS, T7 tag, and TAT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only