Showing: 81 - 100 of 151 results
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Plasmid#177887PurposeBaculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2ADepositorInsertLck (LCK Human)
UseTags10xHis-TEV-4xFRB-SNAP tagExpressionInsectMutationG2APromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-LckAPEX-P2A-EGFP
Plasmid#182826PurposeCre-dependent expression of membrane localized APEXDepositorInsertLckAPEX-P2A-EGFP
UseAAVTagsEGFP reporter (P2A)ExpressionMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-lck-smFLAG-4x6T-WPRE
Plasmid#196414PurposeAstrocytic expression of membrane-targeted FLAG spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorInsertLck-smFLAG
UseAAV; Astrocyte-selectiveTagsLckExpressionMammalianMutationPromoterCAG and GfaABC1DAvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-lck-smMyc-4x6T-WPRE
Plasmid#196415PurposeAstrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorInsertLck-smMyc
UseAAV; Astrocyte-selectiveTagsLckExpressionMammalianMutationPromoterCAG and GfaABC1DAvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-lck-smV5-4x6T-WPRE
Plasmid#196416PurposeAstrocytic expression of membrane-targeted V5 spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorInsertLck-smV5
UseAAV; Astrocyte-selectiveTagsLckExpressionMammalianMutationPromoterCAG and GfaABC1DAvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-flex-lck-smV5-4x6T
Plasmid#196419PurposeCre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorInsertLck-smV5
UseAAV and Cre/Lox; Astrocyte-selectiveTagsLckExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-dDIO-lck-smMyc-4x6T
Plasmid#196421PurposeDre-dependent AAV expression of membrane-targeted Myc spaghetti monster reporter preferentially in astrocytes; astrocyte selectivity generated with 4x6T miRNA targeting cassetteDepositorInsertLck-smMyc
UseAAV; Dre/rox; astrocyte-selectiveTagsLckExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
Membrane EGFP
Plasmid#198056PurposePlasma membrane localizing EGFP using Human LCK proto-oncogene sequenceDepositorInsertLCK membrane EGFP (LCK Synthetic, Human, A. victoria)
UseSea urchin, mammal, zebrafishTagsEGFPExpressionMutationTandem repeat of N-terminal LCK membrane targetin…Promotersp6AvailabilityAcademic Institutions and Nonprofits only -
Membrane mNeonGreen
Plasmid#198057PurposePlasma membrane localizing mNeonGreen using Human LCK proto-oncogene sequenceDepositorInsertLCK membrane mNeonGreen (LCK Human, A.victoria)
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmNeonGreenExpressionMutationTandem repeat of N-terminal LCK membrane targetin…Promotersp6AvailabilityAcademic Institutions and Nonprofits only -
Membrane mCherry
Plasmid#210470PurposePlasma membrane localizing mCherry using Human LCK proto-oncogene sequenceDepositorInsertLCK membrane mCherry (LCK Synthetic, Human, A.victoria)
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmCherryExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
PM-RA
Plasmid#211705PurposeExpression of a dimerization-dependent fluorescent protein (ddFP) subunit (RA) fused with plasma membrane (PM) targetting motif of Lck in mammalian cellsDepositorInsertPM-targetted RA (Lck Synthetic)
UseTagsRAExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLJM1-PM-RA-BlastR
Plasmid#211708PurposeLentiviral expression of a ddFP subunit (RA) fused with the lipid modification motif of lymphocyte-specific protein tyrosine kinase (Lck) for plasma membrane (PM) targetting (PM-RA)DepositorInsertPM-targetted RA (Lck Synthetic)
UseLentiviralTagsRAExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-cSrc
Plasmid#214233PurposeBacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screeningsDepositorInsertcSrc Kinase domain (SRC Human)
UseTags6xHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3-Myr-TYK2
Plasmid#139355PurposeExpression of membrane-anchored human TYK2 protein fused to GFPDepositorInsertTYK2 cDNA with aa 1-16 of human Lck fused to the N-terminus (TYK2 Human)
UseTagsaa 1-16 of human Lck and GFPExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-cAbl
Plasmid#214234PurposeBacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screeningsDepositorInsertABL1 Kinase domain (ABL1 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pEF-HA-TSAd-Y89XF
Plasmid#84559Purposemammalian expression of TSAd-Y89XFDepositorInsertTSAd (SH2D2A Human)
UseTagsHAExpressionMammalianMutationY279F, Y290F, Y305FPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6f
Plasmid#62808PurposepPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6f-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6s
Plasmid#62809PurposepPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6s-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG3-tdT
Plasmid#62810PurposepPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pAAV-i53
Plasmid#92170Purposerecombinant adeno-associated viral plasmid for delivery and expression of ubiquitin variant (i53) that is a selective inhibitor of 53BP1DepositorInserti53
UseAAVTagsFLAGExpressionMammalianMutationUbvG08 with I44A mutation and no terminal GlycinesPromoterCMVAvailabilityIndustry, Academic Institutions, and Nonprofits
Showing: 81 - 100 of 151 results