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pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

Plasmid

#198035

Purpose

Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

SINTBAD (TBKBP1 Human)

Tags

EGFP and GST-TEV

Expression

Insect

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13

Plasmid

#186223

Purpose

For expression of ATG13 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET20b-delta133HtrA2 S142D

Plasmid

#16154

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

His

Expression

Bacterial

Mutation

S142D

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pET20b-delta133HtrA2 S400A

Plasmid

#16155

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

His

Expression

Bacterial

Mutation

S400A

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pFastBac_Dual_GST-TEV-EGFP-TBK1

Plasmid

#187830

Purpose

Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST and TEV-mEGFP-GG Linker

Expression

Bacterial and Insect

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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MFN2-Halo donor plasmid

Plasmid

#239866

Purpose

endogenously tags MFN2 with Halo tag

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

MFN2 (MFN2 Human)

Use

Donor plasmid

Tags

Halo Tag

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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TOMM70-FKBP12-Halo donor plasmid

Plasmid

#239867

Purpose

endogenously tags TOMM70 with Halo tag

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TOMM70 (TOMM70 Human)

Use

Donor plasmid

Tags

Halo Tag

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBMN HA

Plasmid

#188645

Purpose

This is the Lazarou lab's standard retrovirus vector backbone for expression of HA-tag fusions onto the N-terminus of cloned genes

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Type

Empty backbone

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet-1_6xHis-TEV-mCh-NDP52

Plasmid

#187829

Purpose

Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Tags

6xHisTag, TEV, and mCherry

Expression

Bacterial, Insect, and Mamm…

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMX-IY LC3B

Plasmid

#89291

Purpose

For expression of the mammalian Atg8 protein LC3B without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

LC3B (MAP1LC3B Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMX-IY GBRPL1

Plasmid

#89294

Purpose

For expression of the mammalian Atg8 protein GABARAPL1 without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL1 (GABARAPL1 Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMX-IY GBRPL2

Plasmid

#89295

Purpose

For expression of the mammalian Atg8 protein GABARAPL2 without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNAEmptyVector_Puro_T2A_BFP2

Plasmid

#236729

Purpose

Dual sgRNA empty vector expressing BFP with Puromycin selection

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Type

Empty backbone

Use

Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_CTRL_Puro_T2A_BFP2

Plasmid

#236730

Purpose

Dual sgRNA targeting CTRL expressing BFP with Puromycin selection

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

Negative CTRL

Use

Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBMN_HA-TBK1 (E696K)

Plasmid

#199207

Purpose

Used for the expression of TBK1 carrying E696K mutation (SMC Internal No. 1998)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

E696K

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac-GST-TEV-mCherry-TBK1

Plasmid

#198033

Purpose

Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST-TEV and mCherry

Expression

Insect

Mutation

E696K

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1

Plasmid

#187936

Purpose

Plasmid for the expression and purification of GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1327

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Inserts

ATG14 (ATG14 Human)
VPS34 (PIK3C3 Human)
VPS15 (PIK3R4 Human)
BECN1 (BECN1 Human)

Tags

GST-mCherry

Expression

Bacterial

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF1b-His8-TEV-PI31-1-151

Plasmid

#202593

Purpose

Bacterial expression of HsPI31-1-151

Depositor

Brenda Schulman

Article

Kraus et al EMBO Rep. 2023 Aug 3;24(8):e56399. doi:

Insert

PI31 (PSMF1 Human)

Tags

8xHis, TEV

Expression

Bacterial

Mutation

aa 1-151 only

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13(Δ1-198)

Plasmid

#186222

Purpose

For expression of ATG13(Δ1-198) tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Deleted amino acids 1 - 198

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN GBRP

Plasmid

#89293

Purpose

For expression of the mammalian Atg8 protein GABARAP without a tag

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAP (GABARAP Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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