We narrowed to 197 results for: rosa26

1. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

   Plasmid

   #199261

   Purpose

   Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.

   Depositor

   Erik Sontheimer

   Article

   Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

   Insert

   NmeABE8e
   

   Use

   AAV and CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   U1a promoter

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS-HZ54 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26

   Plasmid

   #199265

   Purpose

   Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene

   Depositor

   Erik Sontheimer

   Article

   Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

   Insert

   NmeABE8e
   

   Use

   AAV and CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   U1a

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

   Plasmid

   #86233

   Purpose

   Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   osTir1
   

   Use

   Mouse Targeting

   Tags

   V5 tag

   Expression

   Mammalian

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN114 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt-Rosa26

   Plasmid

   #92143

   Purpose

   Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   osTir1
   

   Use

   Mouse Targeting

   Tags

   V5 tag

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. KL063 - pCAGGS-OsTIR1(F74G)-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

   Plasmid

   #232929

   Purpose

   Targeting vector to introduce an OsTir1(F74G) expressing cassette at the mouse Rosa26 locus using Neomycin selection. AID2 degron system. Use with Addgene #64216 sgRNA.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   OsTir1(F74G)
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Mutation

   changed Phenylalanine 74 to Glycine

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-ROSAf

   Plasmid

   #54323

   Purpose

   TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmid

   Depositor

   Silvestro Conticello

   Insert

   TALEN for the murine ROSA26 locus
   

   Use

   Mouse Targeting and TALEN

   Expression

   Mammalian

   Promoter

   CAGG

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-ROSAr

   Plasmid

   #54324

   Purpose

   TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: GGGAGTCTTCTGGGC. Can be used with the pBS SK mCherryROSAbsr plasmid

   Depositor

   Silvestro Conticello

   Insert

   TALEN for the murine ROSA26 locus
   

   Use

   Mouse Targeting and TALEN

   Expression

   Mammalian

   Promoter

   CAGG

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pR26 CAG/GFP Asc

   Plasmid

   #74285

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Mouse Targeting

   Available Since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pR26 CAG AsiSI/MluI

   Plasmid

   #74286

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pR26 CAG/GFP Dest

    Plasmid

    #74281

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Mouse Targeting

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pR26 GFP Dest

    Plasmid

    #74283

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pR26 CAG/BFP Dest

    Plasmid

    #74282

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pR26 iBFP Dest

    Plasmid

    #74284

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and BFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-R26-FLEX-mStr

    Plasmid

    #135619

    Purpose

    Donor vector for genomic targeting of a CAG-FLEX-mStrawberry cassette to the mouse Rosa26 locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    CAG-FLEX-mStrawberry flanked by Rosa26 homology arms (Gt(ROSA)26Sor Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-RosaR4 KKR mutations

    Plasmid

    #37199

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

    Insert

    FokI (fused to RosaR4 ZFN)
    

    Use

    Zfn expression

    Tags

    HA, ROSA26 eZFNs R4, and SV40 NLS

    Expression

    Mammalian

    Mutation

    I538K, E490K, and H537R

    Promoter

    CMV

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-RosaL6 ELD mutations

    Plasmid

    #37198

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

    Insert

    FokI (fused to Rosa L6 ZFN)
    

    Use

    Zfn expression

    Tags

    HA, ROSA26 eZFNs L6, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Q486E, I499L and N496D

    Promoter

    CMV

    Available Since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGATOR-DTA

    Plasmid

    #64103

    Purpose

    Tet inducible, Gateway adapted ROSA26 targeting Vector with DTA

    Depositor

    Bill Skarnes

    Article

    Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

    Insert

    rTTA (Gt(ROSA)26Sor Synthetic)
    

    Use

    Mouse Targeting; Tet inducible

    Expression

    Mammalian

    Promoter

    ROSA26 endogenous

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGATOR

    Plasmid

    #64102

    Purpose

    Tet inducible, Gateway adapted ROSA26 targeting Vector

    Depositor

    Bill Skarnes

    Article

    Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

    Insert

    rTTA (Gt(ROSA)26Sor Synthetic)
    

    Use

    Mouse Targeting; Tet inducible

    Expression

    Mammalian

    Promoter

    ROSA26 endogenous

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PGKneotpAlox2

    Plasmid

    #13444

    Depositor

    Philippe Soriano

    Article

    Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

    Insert

    PGKneotpAlox2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    Nov. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEM-floxedEGFP-TRI2A

    Plasmid

    #74669

    Purpose

    floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus

    Depositor

    Roberto Giovannoni

    Insert

    floxedEGFP-TRI2A in between the homology regions left (HL) and right (HR) to Rosa26 murine locus
    

    Use

    Mouse Targeting

    Promoter

    pCAGGS

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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