We narrowed to 19,463 results for: MUT

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pCAG-Kir2.1Mut-T2A-tdTomato

Plasmid

#60644

Purpose

Expresses Kir2.1Mut-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Kcnj2 (Kcnj2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

GYG to AAA (aa144-146)

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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betaTrCP mVenus mutant reporter

Plasmid

#192459

Purpose

Fluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.

Depositor

Steven Cappell

Article

Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

Insert

CdC25B

Use

Lentiviral

Tags

SV40 NLS and mVenus

Expression

Mammalian

Mutation

aa198-338 of CDC25B. betaTrCP degron sequence (aa…

Promoter

EF1alpha

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

Plasmid

#162576

Purpose

Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.

Depositor

George Church

Article

Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

Inserts

Lambda Red-Beta
E. coli SSB
EcMutLE32K

Expression

Bacterial

Mutation

E32K mutated to give dominant negative phenotype

Promoter

pBAD

Available Since

May 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_Psyn-sgRNA500

Plasmid

#149576

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CD74-ROS1 nonmutant

Plasmid

#183813

Purpose

Express CD74-ROS1 fusion (C6;R34) in mammalian cells

Depositor

Aaron Hata

Article

Lin et al Clin Cancer Res. 2021 May 15;27(10):2899

Inserts

ROS Proto-Oncogene 1, Receptor Tyrosine Kinase (ROS1 Human)
CD74 molecule (CD74 Human)

Use

Lentiviral

Tags

The fusion protein of CD74 exon 1-6 and ROS1 exon…

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET30a-His6-ABP-LOXCATmut

Plasmid

#139972

Purpose

A control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)

Depositor

Vamsi Mootha

Article

Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

Insert

A fusion of lactate oxidase from A. viridans and catalase from E. coli

Tags

His6

Expression

Bacterial

Mutation

Mutation in H265A and R268A in LOX (used original…

Promoter

T7

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-RosaR4 KKR mutations

Plasmid

#37199

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

Insert

FokI (fused to RosaR4 ZFN)

Use

Zfn expression

Tags

HA, ROSA26 eZFNs R4, and SV40 NLS

Expression

Mammalian

Mutation

I538K, E490K, and H537R

Promoter

CMV

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-RosaL6 ELD mutations

Plasmid

#37198

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

Insert

FokI (fused to Rosa L6 ZFN)

Use

Zfn expression

Tags

HA, ROSA26 eZFNs L6, and SV40 NLS

Expression

Mammalian

Mutation

Q486E, I499L and N496D

Promoter

CMV

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pALOD4 non-binding mutant

Plasmid

#111027

Purpose

Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)

Depositor

Arun Radhakrishnan

Article

Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

Insert

Domain 4 of Anthrolysin O

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: S404C, C472A, G501A, T502A, T503A, L5…

Promoter

T7

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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L0I80_Bxb1mut(noBsaI)_GG1-GG4

Plasmid

#219715

Purpose

Level 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.

Depositor

Jennifer Nemhauser

Insert

GG1-Bxb1(noBsaI0)-GG4

Use

Synthetic Biology

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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