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We narrowed to 11,126 results for: hal

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Halo-WIPI2B R108E

Plasmid

#176004

Purpose

Expresses WIPI2B (R108E) fused to Halo in mammalian cells

Depositor

Erika Holzbaur

Article

Stavoe et al Elife. 2019 Jul 16;8. pii: 44219. doi: 1

Insert

WIPI2B (R108E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Arginine 108 to Glutamate

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CEP41_HaloTag9

Plasmid

#169331

Purpose

Expression of HaloTag9 located at the microtubules of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CEP41-HaloTag9 (CEP41 Human)

Use

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Na0.5

Plasmid

#198634

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Na0.5

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET.HaloTag.v7.CoOp.Cys-less

Plasmid

#195487

Purpose

Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Insert

HaloTag.v7

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C61T, C262V

Promoter

T7

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-HaloLICNot

Plasmid

#140185

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, Halo-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

Halo

Expression

Mutation

Promoter

T7

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pBSc polyA + Shal2

Plasmid

#16144

Depositor

Larry Salkoff

Article

Butler et al Science. 1989 Feb 17. 243(4893):943-7.

Insert

Shal (Shal Fly)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

N/A

Promoter

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
Id2-Halo donor

Plasmid

#113116

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Id2 donor region including homology arms and HaloTag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mNG:HaloTag

Plasmid

#179785

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Keith Gull, Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG::HaloTag

Use

Unspecified

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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YIplac211-GGA2-HaloTagx2

Plasmid

#140150

Purpose

Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-HaloTagx2

Depositor

Benjamin Glick

Article

Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

Insert

GGA2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B L69E

Plasmid

#175026

Purpose

Expresses WIPI2B (L69E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (L69E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Leucine 69 to Glutamate (CTA to GAA)

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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