We narrowed to 8,867 results for: BLI;

1. pUltra-Smurf-luc-RTTA3

   Plasmid

   #59438

   Purpose

   silence doxycyclin inducible pUltra-dox (also expresses luciferase)

   Depositor

   Yildirim Dogan, Kitai Kim

   Inserts

   luciferase
   Reverse TET-Transactivator 3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   loxP5171

   Promoter

   Available since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHAGE2-RSV-jGCaMP7s-W

   Plasmid

   #126690

   Purpose

   Mammalian calcium sensor protein.

   Depositor

   Darrell Kotton

   Insert

   jGCaMP7s
   

   Use

   Lentiviral

   Tags

   6xHIS, T7, Xpress

   Expression

   Mutation

   A52V, A317L

   Promoter

   RSV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV Tor1a-V5

   Plasmid

   #175135

   Purpose

   Lentiviral expression of Tor1a-Myc

   Depositor

   Larry Gerace

   Insert

   Tor1a (Tor1a Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHAGE2-RSV-jGCaMP7f-W

   Plasmid

   #126691

   Purpose

   Mammalian calcium sensor protein.

   Depositor

   Darrell Kotton

   Insert

   jGCaMP7f
   

   Use

   Lentiviral

   Tags

   6xHIS, T7, Xpress

   Expression

   Mutation

   H78K, A317L

   Promoter

   RSV

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBluescript mouse ptc

   Plasmid

   #58699

   Purpose

   full length mouse ptc cDNA

   Depositor

   Matthew Scott

   Insert

   ptc (Ptch1 Mouse)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pMC6-TEFkFLP(LYS2)

   Plasmid

   #49458

   Purpose

   overespression of Flp step-arrest mutant in yeast, LYS2 selection

   Depositor

   Marc Gartenberg

   Insert

   Flp(H305L) (flp Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H305L, ‘step-arrest’ mutant,

   Promoter

   TEF1

   Available since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJK266

   Plasmid

   #73326

   Purpose

   Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag

   Depositor

   T. J. Kappock

   Insert

   violacein biosynthesis protein VioD
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIC81

   Plasmid

   #63065

   Purpose

   Mammalian expression of EGFP-TEV-S-tag PMFI for protein localization and affinity purification (LAP)

   Depositor

   Iain Cheeseman

   Insert

   PMFI, IMAGE clone 6148250 (PMF1 Human)
   

   Use

   Tags

   EYFP and TEV-S

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PB CAG-L-bpA-SV40pA-L-td-sfGFP

   Plasmid

   #133575

   Purpose

   CAG tdGFP cre/lox reporter piggyBac transposon (bpA 1x SV40pA stop)

   Depositor

   Mario Capecchi

   Insert

   Tandem dimer superfolder GFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PTEN-VC

    Plasmid

    #89332

    Purpose

    expresses hPTEN fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Insert

    PTEN1 (PTEN Human)
    

    Use

    Tags

    C-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. B0030m_BC

    Plasmid

    #74394

    Purpose

    MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0030 to adjust spacing in MC system. [B:B0030m:C]

    Depositor

    Douglas Densmore

    Insert

    RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSBtet-Bla/GFP

    Plasmid

    #198221

    Purpose

    Transposon vector (Addgene #60510) for doxycycline-inducible expression of EGFP

    Depositor

    Eric Davis

    Insert

    EGFP
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK690

    Plasmid

    #72380

    Purpose

    Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)

    Depositor

    T. J. Kappock

    Insert

    glutaredoxin-4
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. H2B CFP

    Plasmid

    #198059

    Purpose

    mCerulean fused to human H2B protein

    Depositor

    Amro Hamdoun

    Insert

    H2B
    

    Use

    Synthetic Biology; Sea urchin, mammal, zebrafish

    Tags

    mCerulean

    Expression

    Mutation

    Promoter

    sp6

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK409

    Plasmid

    #72423

    Purpose

    Produces Acetobacter aceti 1023 Est14, C-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    arylesterase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK405

    Plasmid

    #72418

    Purpose

    Produces Acetobacter aceti 1023 Est13, N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    arylesterase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK403

    Plasmid

    #72416

    Purpose

    Produces Acetobacter aceti 1023 Est07, N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    arylesterase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MAC045-C12

    Plasmid

    #58753

    Purpose

    bacterial expression of PDB 4NXJ

    Depositor

    Cheryl Arrowsmith

    Insert

    PDB:4NXJ
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    T7

    Available since

    Aug. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. MB121 (Nf2 BBA)

    Plasmid

    #12170

    Depositor

    Tyler Jacks

    Insert

    Nf2 BBA (Nf2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    BBA: dominant negative Nf2, 7 Blue Box residues c…

    Promoter

    Available since

    July 7, 2006

    Availability

    Academic Institutions and Nonprofits only

20. KBB610

    Plasmid

    #185111

    Purpose

    Pom152 full repeat domain in pEX-N-His for bacterial expression and purification

    Depositor

    Ken Belanger

    Insert

    POM152
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Pom152 Repeats-6His

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 371-Bc

    Plasmid

    #97028

    Purpose

    yORF-tev-His6

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    His6 tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. 371-B

    Plasmid

    #97027

    Purpose

    His6-tev-yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MIGR1-PREP1

    Plasmid

    #78147

    Purpose

    retroviral expression of PREP1 and GFP

    Depositor

    Francesco Blasi

    Insert

    PREP1 (PKNOX1 Human)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pHH0103 SMURF2 WW domain #3

    Plasmid

    #104226

    Purpose

    Bacterial expression of WW domain #3 from SMURF2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    SMURF2 WW domain #3 (SMURF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHH0103 SMURF2 WW domain #2

    Plasmid

    #104225

    Purpose

    Bacterial expression of WW domain #2 from SMURF2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    SMURF2 WW domain #2 (SMURF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHH0103 SMURF2 WW domain #1

    Plasmid

    #104224

    Purpose

    Bacterial expression of WW domain #1 from SMURF2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    SMURF2 WW domain #1 (SMURF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

    Plasmid

    #137859

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pE-SUMO CRYGB

    Plasmid

    #80751

    Purpose

    Expression of human gamma B-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGB (CRYGB Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mGeos2-VEL-Lifeact-7

    Plasmid

    #54817

    Purpose

    Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mGeos2-VEL-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

30. pCB94

    Plasmid

    #69743

    Purpose

    Retroviral expression of PP2A beta

    Depositor

    Iain Cheeseman

    Insert

    PP2A beta (PTPA Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pJK365

    Plasmid

    #72404

    Purpose

    Produces Acetobacter aceti 1023 Est01

    Depositor

    T. J. Kappock

    Insert

    acyl-CoA thioester hydrolase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. 4I79

    Plasmid

    #51319

    Depositor

    Cheryl Arrowsmith

    Insert

    4I79 (NUP43 Human)
    

    Use

    Baculovirus expression

    Tags

    His

    Expression

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available since

    Feb. 24, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

33. pHH0103 WBP4 WW domain #2

    Plasmid

    #104253

    Purpose

    Bacterial expression of WW domain #2 from WBP4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    WBP4 WW domain #2 (WBP4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mEos-A69V-N1

    Plasmid

    #54754

    Purpose

    Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mEos A69V, V123T, T158H

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

35. pE-SUMO CRYGC

    Plasmid

    #80754

    Purpose

    Expression of human gamma C-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGC (CRYGC Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFA6a-SRM (sortase recognition motif)-kanMX6

    Plasmid

    #54489

    Purpose

    PCR based C-terminal SRM tagging in yeast

    Depositor

    Eri Sakata

    Insert

    SRM (sortase-recognition motif)
    

    Use

    Yeast genome targeting

    Tags

    kanMX6

    Expression

    Mutation

    Promoter

    Available since

    Aug. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pD434-SR rat Alpha-synuclein

    Plasmid

    #89076

    Purpose

    bacterial expression of rat Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pAAV-2xcHS4-cjSST-E2-CMVmini-3xHA-mScarlet3-miR-WPRE-hGHpA

    Plasmid

    #240048

    Purpose

    Expression of RFP in cortical SST interneurons in marmoset

    Depositor

    Guoping Feng

    Insert

    mScarlet3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMVmini

    Available since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV gfaABC1D:NES-jRGECO1a

    Plasmid

    #239682

    Purpose

    Red calcium indicator jRGECO1a with a nuclear export signal expressed under an astrocyte-specific promoter.

    Depositor

    Marianne Fyhn

    Insert

    jRGECO1a
    

    Use

    AAV

    Tags

    NES

    Expression

    Mammalian

    Mutation

    Promoter

    gfaABC1D

    Available since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAVS-sA-NeoR

    Plasmid

    #240383

    Purpose

    For insertion into AAV Safe Harbor site, provides G418 resistance

    Depositor

    Ronald Hart

    Insert

    NeoR (neoR )
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    None. Driven by PPP1R12C promoter after homologo…

    Available since

    July 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pN_UBQ10/mApple

    Plasmid

    #140405

    Purpose

    mApple expression regulated by the A. thaliana UBQ10 promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

    Depositor

    Mathias Pribil

    Insert

    mApple
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pU6:2-U6:3-tevopreq1

    Plasmid

    #190910

    Purpose

    Expression of nicking sgRNA and epegRNA under control of separate Drosophila U6:2 and U6:3 promoters. Can be used to generate transgenic flies with vermillion+ selection.

    Depositor

    Norbert Perrimon

    Insert

    Empty Backbone
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSG7163 pLIBT7_SNAPtag-3C-8His

    Plasmid

    #232622

    Purpose

    Expression vector for SNAPtag (carboxy terminal 3C-8His tag)

    Depositor

    Stephan Gruber

    Insert

    SNAPtag-3C-8His
    

    Use

    Tags

    3C-8His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCAGGS-MTS(S17/43A)-EGFP-V5

    Plasmid

    #200935

    Purpose

    expresses MTS(S17/43A)-EGFP-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    EGFP
    

    Use

    Tags

    V5 and mitochondrial targeting sequence (MTS)

    Expression

    Mammalian

    Mutation

    S17A, S43A in MTS

    Promoter

    CAG

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCfb13198

    Plasmid

    #219873

    Purpose

    The base plasmid of TUNEYALI forTF08

    Depositor

    Irina Borodina

    Insert

    Contains gRNA targeting TF08 ( YALI1_D33640g) and homologous arm matching TF08
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pMT-Ex

    Plasmid

    #231106

    Purpose

    Inducible protein expression in insect cell culture. Stable cell line generation.

    Depositor

    Michael Jeltsch

    Type

    Empty backbone

    Use

    Tags

    6xHis and V5

    Expression

    Insect

    Mutation

    Promoter

    Drosophila metallothionein promoter, MT

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. PB-H2A-LSSmOrange2-2A-ERK-KTR-LSS-SGFP2

    Plasmid

    #129629

    Purpose

    Visualization of ERK activity

    Depositor

    Dorus Gadella

    Insert

    PB-H2A-LSSmOrange2-2A-ERK-KTR-LSS-SGFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAG Retro Hygro Empty

    Plasmid

    #234459

    Purpose

    Retroviral vector for cloning and expressing your gene of interest. Hygromycin selection.

    Depositor

    Melina Fan, Addgene Research Program

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

49. 1203N-CMV-dDiCas7-11-NLS-bGHpolyA

    Plasmid

    #221537

    Purpose

    Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain

    Depositor

    Omar Akbari

    Insert

    DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. 1203AD-CMV-Cas7.1-N152A-mutant

    Plasmid

    #221543

    Purpose

    ffector expresses Cas7.1 domain (truncated from DiCas7-11), N to A mutation at amino acid 152

    Depositor

    Omar Akbari

    Insert

    Truncated DiCas7-11
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge