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We narrowed to 1,229 results for: Aspa

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Showing: 981 - 1000 of 1229 results

  2. N229A Lo-MetQ

    Plasmid
    #118268
    Purpose
    N229A Mutation in Methionine Binding Protein (MetQ) used to solve crystal structure
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Insert
    MetQ (N229A) (metQ E. coli)
    Tags
    10x His Tag and Enterokinase Cleavage Site
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 229 to Alanine (Ala)
    Promoter
    T7 Promoter
    Available Since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    118268-plasmid-map-sequence-id-228534

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  5. pJK524

    Plasmid
    #71580
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes asparagine-347 to alanine
    Promoter
    T7
    Available Since
    April 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    71580-plasmid-map-sequence-id-130939

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  6. pJK517

    Plasmid
    #71579
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)
    Depositor
    T. J. Kappock
    Article
    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34
    Insert
    succinyl-CoA:acetate CoA-transferase
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changes glutamate-435 to aspartate
    Promoter
    T7
    Available Since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71579-plasmid-map-sequence-id-130938

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  12. pSPObooster

    Plasmid
    #216160
    Purpose
    Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains
    Depositor
    Grant Brown
    Article
    Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978
    Inserts
    MKT1(30G) (MKT1 Budding Yeast)
    RME1(ins-308A) (RME1 Budding Yeast)
    Expression
    Yeast
    Mutation
    MKT1 gene containing an aspartic acid to glycine …
    Promoter
    endogenous promoter
    Available Since
    May 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    216160-plasmid-map-sequence-id-433867

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  14. HF-PX459 (V2)

    Plasmid
    #118632
    Purpose
    Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance
    Depositor
    Mike McGrew
    Article
    Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10
    Insert
    hSpCas9-HF1-2A-Puro V2.0
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Asparagine 497 to Alanine, Arginine 661 to Alanin…
    Promoter
    Cbh
    Available Since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    118632-plasmid-map-sequence-id-230217

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  18. pMini-CMV-NLS-dead R-IscB (D60A, H269A)-NLS_T2A_mCherry_U6-ωRNA

    Plasmid
    #246430
    Purpose
    All-in-one plasmid. Expresses R-IscB in mammalian cells. Dead mutations included.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    mCherry
    ωRNA
    Tags
    SV40 NLS, Thosea asigna virus 2A peptide, and nuc…
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 60 to Alanine, changed Hist…
    Promoter
    CMV and U6
    Available Since
    Oct. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    246430-plasmid-map-sequence-id-490873

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  19. pMXs-IP- SCAT3.2

    Plasmid
    #172497
    Purpose
    SCAT3.2 is fluorescent reporter (FRET probe) for cell survival/apoptosis with Caspase-3 sensitive sequence which can be introduced to mammalian cells by retrovirus vector.
    Depositor
    David Scadden
    Article
    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:
    Insert
    Amcyan
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Promoter
    MLV LTR
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172497-plasmid-map-sequence-id-340999

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Showing: 981 - 1000 of 1229 results