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Ptrc::galP_NS1

Plasmid

#101843

Purpose

drives expression of galP transporter from NS1 locus

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

Ptrc promoter driving GalP transporter expression

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pJRL2 GAL1(TATA-C2)pr VYFP (EB#1577)

Plasmid

#14851

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

GAL1 promoter TATA-C2 (GAL1 Budding Yeast)

Use

Tags

VYFP

Expression

Yeast

Mutation

TATA-C2. TATA box mutated from TATATAAG to TCTATA…

Promoter

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pJRL2 GAL1(TATA-C2)pr ECFP (EB#1578)

Plasmid

#14852

Depositor

Erin O'Shea

Article

Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

Insert

GAL1 promoter TATA-C2 (GAL1 Budding Yeast)

Use

Tags

ECFP

Expression

Yeast

Mutation

TATA-C2. TATA box mutated from TATATAAG to TCTATA…

Promoter

Available since

May 25, 2007

Availability

Academic Institutions and Nonprofits only
pLenti-dCas9-KRAB-gRNA-TRE-blast

Plasmid

#201152

Purpose

Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

Depositor

Moshe Szyf

Inserts

dCas9-KRAB
gRNA-TRE

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mutation

gRNA sequence: TACGTTCTCTATCACTGAT

Promoter

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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MLV Gag-RFP

Plasmid

#1814

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Gag (gag MuLV)

Use

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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Friend MLV Env YFP

Plasmid

#1820

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Envelope (env MuLV)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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Lenti-Cas9-gRNA-GFP

Plasmid

#124770

Purpose

3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

Depositor

Jason Sheltzer

Article

Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-Halo

Plasmid

#200197

Purpose

Expression for SV40 NLS

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

3X SV40 NLS-Halo

Use

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCambia-PUP-IT

Plasmid

#186478

Purpose

Expresses POI-PUP-IT fusion in Plants

Depositor

Jen Sheen

Article

Ye et al Nature. 2022 Sep 14. pii: 10.1038/s41586

Insert

FLAG-Pup(E)

Use

Tags

Triple Flag Tag

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pE-FLP

Plasmid

#45978

Depositor

Keith Shearwin, Drew Endy

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Insert

flp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

flp driven by the constitutive promoter pE from p…

Promoter

pE (from phage P2)

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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Beta-arrestin2 GFP WT

Plasmid

#35411

Depositor

Robert Lefkowitz

Article

Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

Insert

βarrestin2 (Arrb2 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pEF_dCas9

Plasmid

#68416

Purpose

Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A/H841A

Promoter

human EF1[alpha]

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pBbAW4k-loxP-TT-loxP-mRFP1

Plasmid

#134405

Purpose

Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

Depositor

Mary Dunlop

Article

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

W4-loxP-TT-loxP-mRFP1

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

W4 (modified T7A1)

Available since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-LambdaN-NbALFA

Plasmid

#207627

Purpose

Expresses LambdaN-NbALFA (ALFA-tag nanobody) fusion protein in mammalian cells (with a CMV promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

LambdaN-NbALFA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

pCMV

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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TfR-pHuji

Plasmid

#61505

Purpose

Human transferrin receptor tagged with pH sensitive red fluorescent protein pHuji

Depositor

David Perrais

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

Transferrin receptor (TFRC Human)

Use

Tags

pHuji

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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NES-Halo

Plasmid

#200201

Purpose

Expression of 3X HIV NES

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

3X HIV Rev NES-Halo

Use

Tags

Halo

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-KIRIN1

Plasmid

#107414

Purpose

Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAM5409

Plasmid

#132662

Purpose

RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Inserts

aadA
YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PaadA and PconII

Available since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAM5505

Plasmid

#132664

Purpose

pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Insert

MobA

Use

Tags

Expression

Bacterial

Mutation

please see depositor comments below

Promoter

p1,p3

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBAD-LSSmCherry1

Plasmid

#89986

Purpose

Long stokes-shift variant of red fluorescent protein mCherry

Depositor

Robert Campbell

Article

Shen et al PLoS One. 2017 Feb 27;12(2):e0171257. do

Insert

LSSmCherry1

Use

Tags

6xHis-T7-Xpress

Expression

Bacterial

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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