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We narrowed to 2,011 results for: cas9 expression vector

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Showing: 981 - 1000 of 2011 results

  3. pCfB6371

    Plasmid
    #106153
    Purpose
    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6630, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106153-plasmid-map-sequence-id-205323

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  4. pCfB6677

    Plasmid
    #106155
    Purpose
    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6632, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106155-plasmid-map-sequence-id-206140

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  5. pCfB6684

    Plasmid
    #106154
    Purpose
    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106154-plasmid-map-sequence-id-206237

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  6. pCfB6682

    Plasmid
    #106152
    Purpose
    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106152-plasmid-map-sequence-id-205326

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  7. pCfB6679

    Plasmid
    #106158
    Purpose
    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6638, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106158-plasmid-map-sequence-id-206236

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  8. pCfB6681

    Plasmid
    #106157
    Purpose
    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6637, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106157-plasmid-map-sequence-id-206132

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  9. pMJ922

    Plasmid
    #78312
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Tags
    HA-2xNLS-EGFP-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78312-plasmid-map-sequence-id-457381

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  10. pSECC

    Plasmid
    #60820
    Purpose
    3rd generation vector. Expresses a sgRNA of interest, Cas9 and Cre
    Depositor
    Tyler Jacks
    Article
    Sanchez-Rivera et al Nature. 2014 Oct 22. doi: 10.1038/nature
    Inserts
    Cas9
    Cre
    Use
    CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    BsmBI site eliminated by C->A; E308G
    Promoter
    EFS and EFS (after Cas9-2A)
    Available Since
    Nov. 19, 2014
    Availability
    Academic Institutions and Nonprofits only
    60820-plasmid-map-sequence-id-371636

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  11. MSP469

    Plasmid
    #65771
    Purpose
    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65771-plasmid-map-sequence-id-112653

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  12. MSP680

    Plasmid
    #65772
    Purpose
    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E, R1335Q, and T1337R mutations in Cas9
    Promoter
    CMV & T7
    Available Since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65772-plasmid-map-sequence-id-112654

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  13. pMJ923

    Plasmid
    #78313
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Tags
    HA-2xNLS-mCherry-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78313-plasmid-map-sequence-id-148391

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  16. pTJK459

    Plasmid
    #138008
    Purpose
    Empty sgRNA expression vector for expression of sgRNA for Sp Cas9
    Depositor
    Tami Kingsbury
    Type
    Empty backbone
    Use
    Lentiviral
    Mutation
    U-A flip and hairpin extension: cgtttAagagctaTGCT…
    Available Since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138008-plasmid-map-sequence-id-275002

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  17. pDM026

    Plasmid
    #216808
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216808-plasmid-map-sequence-id-429385

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  19. pDM028

    Plasmid
    #216809
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and hph selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216809-plasmid-map-sequence-id-430135

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  20. pDM068

    Plasmid
    #216811
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216811-plasmid-map-sequence-id-430142

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Showing: 981 - 1000 of 2011 results