We narrowed to 148,482 results for: ins;

1. pMSP1E1D1

   Plasmid

   #161605

   Purpose

   Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

   Insert

   MSP1E1D1
   

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeat of h…

   Promoter

   T7

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDonor14-N7HELIX-KanR

   Plasmid

   #190663

   Purpose

   Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Expression

   Bacterial

   Promoter

   NA

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-HxB.L

   Plasmid

   #65414

   Purpose

   contains complete cDNA for TNC large splice variant flanked by NotI-KpnI

   Depositor

   Harold Erickson

   Article

   Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

   Insert

   TNC large splice variant (TNC Human)
   

   Expression

   Bacterial

   Available Since

   June 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYMA1_SidK_mCH

   Plasmid

   #184906

   Purpose

   Legionella pneumophila SidK (1-278) - mCherry

   Depositor

   John Rubinstein

   Article

   Maxson et al J Cell Biol. 2022 Mar 7;221(3). pii: 212

   Insert

   SidK (1-278) (sidK Legionella pneumophila)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSP1E2D1

   Plasmid

   #161606

   Purpose

   Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

   Insert

   MSP1E2D1
   

   Tags

   7-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1D1; contains repeat of h…

   Promoter

   T7

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAraH6HATT

   Plasmid

   #63901

   Purpose

   For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.

   Depositor

   Carlos Barbas

   Article

   Barbas et al Methods, A companion to Methods in Enzym

   Insert

   TT Fab
   

   Tags

   6xHis and HA tag

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSC101-GFPmut2-mScarlet-I TAA

   Plasmid

   #208187

   Purpose

   nonsense mistranslation, TAA stop

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Expression

   Bacterial

   Mutation

   Insertion 6TAA*(stop) 7AGT(Ser)

   Promoter

   Ptet+dnaK P1

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVTU_260_BirA

   Plasmid

   #63682

   Purpose

   Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

   Insert

   BirA
   

   Tags

   6X Histidine and TEV cleavage site

   Expression

   Yeast

   Promoter

   ADH1/yeast

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDD284

   Plasmid

   #66825

   Purpose

   TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   TagRFP-T-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized TagRFP-T

   Expression

   Worm

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pDD285

    Plasmid

    #66826

    Purpose

    mKate2^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    mKate2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized mKate2

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pBS-HxB.S

    Plasmid

    #65415

    Purpose

    contains complete cDNA for TNC small splice variant flanked by NotI-KpnI

    Depositor

    Harold Erickson

    Article

    Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

    Insert

    tenascin C small splice variant (TNC Human)
    

    Expression

    Bacterial

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cmv d0 dPspCas13b-GGS-NYTHDF1

    Plasmid

    #119855

    Purpose

    Cmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)

    Depositor

    Bryan Dickinson

    Article

    Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

    Insert

    dCas13b-GGS-NYTHDF1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUASp YFP Rabx6 WT

    Plasmid

    #53479

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rabx6 (RabX6 Fly)
    

    Tags

    YFP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    May 12, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pMSP2

    Plasmid

    #26282

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP2
    

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    synthetic gene of dimer of deletion mutant (1-43)…

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pSC101-GFPmut2-mScarlet-I TAG

    Plasmid

    #208188

    Purpose

    nonsense mistranslation, TAG stop

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Expression

    Bacterial

    Mutation

    Insertion 6TAG*(stop) 7AGT(Ser)

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSC101-GFPmut2-mScarlet-I TGA

    Plasmid

    #208189

    Purpose

    nonsense mistranslation, TGA stop

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Expression

    Bacterial

    Mutation

    Insertion 6TGA*(stop) 7AGT(Ser)

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSC101-GFPmut2-mScarlet-I TGATC

    Plasmid

    #208190

    Purpose

    nonsense mistranslation, TGA stop variant 2

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Expression

    Bacterial

    Mutation

    Insertion 6TGA*(stop) 7TCT(Ser)

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSC101-GFPmut2-mScarlet-I T7

    Plasmid

    #208191

    Purpose

    frameshift mistranslation, T7

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Expression

    Bacterial

    Mutation

    Frameshift insertion 6TTTTTTT

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSC101-GFPmut2-mScarlet-I A5

    Plasmid

    #208192

    Purpose

    frameshift mistranslation, A5

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Expression

    Bacterial

    Mutation

    Frameshift insertion 6AAAAA

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSC101-GFPmut2-mScarlet-I A4

    Plasmid

    #208193

    Purpose

    frameshift mistranslation, A4

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Expression

    Bacterial

    Mutation

    Frameshift insertion 6AAAA

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge