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pHH0103_SH3BP4-1/1

Plasmid

#91532

Purpose

Protein expression and purification of human SH3 domain construct SH3BP4-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3BP4-1/1 (SH3BP4 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SORBS2-1/3

Plasmid

#91387

Purpose

Protein expression and purification of human SH3 domain construct SORBS2-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SORBS2-1/3 (SORBS2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_VAV1-1/2

Plasmid

#91355

Purpose

Protein expression and purification of human SH3 domain construct VAV1-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

VAV1-1/2 (VAV1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRS313-PRP8

Plasmid

#90964

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Promoter

native

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_STAM-1/1

Plasmid

#91428

Purpose

Protein expression and purification of human SH3 domain construct STAM-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

STAM-1/1 (STAM Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pUC 3GLA UAS HAi

Plasmid

#71763

Purpose

gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tag

Depositor

Matthias Soller

Article

Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

Type

Empty backbone

Expression

Insect

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 dSesn (1187)

Plasmid

#66919

Purpose

bacterial expression of dSesn

Depositor

Michael Karin, Jun Hee Lee

Article

Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

Insert

Sesn (Sesn Fly)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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sh-Myocardin

Plasmid

#100768

Purpose

expression of shRNA targeting MYOCD

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

sh-Myocardin

Use

RNAi

Promoter

H1

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSB819-PKR-hum-LFA

Plasmid

#20051

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Mutation

S492A

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pHH0103_PACSIN3-1/1

Plasmid

#91335

Purpose

Protein expression and purification of human SH3 domain construct PACSIN3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PACSIN3-1/1 (PACSIN3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF1-ERBB4 (E563K)

Plasmid

#29541

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-RsACR_995-EYFP

Plasmid

#103772

Purpose

Anion channelrhodopsin from Rhodomonas salina (CCMP1319) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin RsACR_995

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GcACR_457-EYFP

Plasmid

#103137

Purpose

Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin GcACR_457

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pOT2 3GLA

Plasmid

#71764

Purpose

gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, chloramphenicol resistant

Depositor

Matthias Soller

Article

Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

Type

Empty backbone

Expression

Insect

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pRS426-TUB1-internal6xHis (1-440)

Plasmid

#60391

Purpose

Expression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4), amino a…

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
MDH1-PGK-FLAG-TAT-SIVmac251

Plasmid

#35979

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

transactivating protein

Use

RNAi and Retroviral

Tags

Flag

Expression

Mammalian

Mutation

SIV strain mac251

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2801

Plasmid

#63656

Purpose

pCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-217

Plasmid

#26333

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-217 (Mir217 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pFP122

Plasmid

#21669

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
MPDZ_PDZ_7

Plasmid

#103891

Purpose

Protein expression and purification of Multiple PDZ protein PDZ7 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MPDZ (MPDZ Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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