We narrowed to 11,712 results for: NSI

1. NK66

   Plasmid

   #176606

   Purpose

   To measure Syk intensity in response to ligand clustering using DNA origami

   Depositor

   Meghan Morrissey

   Article

   Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

   Insert

   Syk-BFP (Syk Mouse, Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   n/a

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. TNXXL-CTS68

   Plasmid

   #48343

   Purpose

   FRET reporter for Ca2+ inside primary cilia

   Depositor

   Takanari Inoue

   Article

   Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

   Insert

   CTS68
   

   Tags

   TN-XXL

   Expression

   Mammalian

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pDRF1-GW AKAR3-EV-NR

   Plasmid

   #182534

   Purpose

   Expresses the non responsive PKA FRET sensor AKAR3-EV (T506A) in budding yeast

   Depositor

   Bas Teusink

   Article

   Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

   Insert

   AKAR3-EV-NR
   

   Expression

   Yeast

   Mutation

   T506A mutation

   Promoter

   PMA1

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. P442

   Plasmid

   #139728

   Purpose

   Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz, promoter is ABA responsive

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pMOD_D_pAtRAB18:NnLuz:tAtuOcs
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   AtRAB18

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRE/WT SP with 5'UTR

   Plasmid

   #89330

   Purpose

   Inducible expression of WT spastin M1 and M87 in mammalian cells

   Depositor

   Peter Baas

   Article

   Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

   Insert

   spastin (SPAST Human)
   

   Expression

   Mammalian

   Promoter

   Tet-responsive P tight

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-3×FLAG-SLC35A1

   Plasmid

   #186282

   Purpose

   transient expression of N-terminal 3×FLAG tagged SLC35A1 isoform a in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

   Insert

   SLC35A1 (SLC35A1 Human)
   

   Tags

   3×FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBRN168

   Plasmid

   #79668

   Purpose

   DsREDmonomer-based BiFC vector for transient expression of RN (1-168) in plants.

   Depositor

   Yutaka Kodama, Masamitsu Wada

   Article

   Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

   Type

   Empty backbone

   Use

   Reporter plasmid

   Tags

   DsRED-monomer (1–168)/RN168

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2iU6m5Hoxa13bE4PCh(#765)

   Plasmid

   #184102

   Purpose

   cyclofen-inducible fish Hoxa13b activation via zebrafish transient transgenesis or mRNA synthesis

   Depositor

   Sophie Vriz

   Insert

   5myc-ZfHoxa13b-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes) + in vitro tra…

   Tags

   5xMyc

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi+SP6

   Available Since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA

   Plasmid

   #162802

   Purpose

   STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient expression

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-BsmBI-HA
   

   Use

   Piggybac

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB4C5_pBen-BenR

    Plasmid

    #128127

    Purpose

    Expressing BenR transcription factor gene under control of benzoate responsive promoter (pBen) in a feedback loop.

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    BenR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pBen

    Available Since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMB-TLEr

    Plasmid

    #61188

    Purpose

    Fluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MtRAB7A2
    

    Tags

    mCherry

    Expression

    Plant

    Promoter

    MtBCP1

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUPD2+NtEUt

    Plasmid

    #170883

    Purpose

    Phytobrick - NtEUt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    NtEUt
    

    Expression

    Plant

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVEG17*

    Plasmid

    #111856

    Purpose

    Xylose-responsive Gal3p-Syn4.1 under control of native GAL1p (promoter)

    Depositor

    Nikhil Nair

    Article

    Endalur Gopinarayanan et al Nat Commun. 2018 Mar 26;9(1):1233. doi:

    Insert

    GAL1p-GAL3-Syn4.1-TEFt
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    D68N, V69M, A109V, I271L

    Promoter

    GAL1p

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6_(GLuc)_sgRNA

    Plasmid

    #68422

    Purpose

    Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Gluc sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUCBB-lacZa

    Plasmid

    #32563

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    LacZ-alpha
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Promoter

    Consitutive lac

    Available Since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

    Plasmid

    #162810

    Purpose

    STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    BsmBI_Intein_DD
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXW101Plux2-gfp

    Plasmid

    #160819

    Purpose

    AHL-responsive single-layered circuit

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

    Insert

    PJ23101-30luxR-t-Plux2-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PJ23101, Plux2

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTEI134

    Plasmid

    #126071

    Purpose

    Vector for expression of plastid targeted YFP1-10 (T203Y) in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    Chloroplastic FNR transit peptide
    

    Use

    Binary

    Tags

    YFP1-10

    Expression

    Plant

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 Flag-Sesn3

    Plasmid

    #61869

    Purpose

    Transient overexpression of Flag-Sesn3 (mouse)

    Depositor

    Ming Li

    Article

    Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

    Insert

    Sesn3 (Sesn3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

20. CD4_LD 0_TEV

    Plasmid

    #58870

    Purpose

    MESA protease chain with CD4 ectodomain and no linker domain

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA protease chain with CD4 ectodomain and TEV protease
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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