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Bcr DH domain NE/AA pGEX

Plasmid

#38180

Depositor

Nora Heisterkamp

Publication

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR DH domain N689A/E690A (BCR Human)

Tags

GST

Expression

Bacterial

Mutation

BCR DH domain N689A/E690A

Available since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
BRCA1-(1234-1443)

Plasmid

#35078

Depositor

Cheryl Arrowsmith

Publication

Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

Insert

BRCA1 (BRCA1 Human)

Tags

His

Mutation

Contains aa 1234-1443

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
NHA-PAZ(aa1-480)

Plasmid

#21530

Depositor

Edward Chan

Publication

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

NHA

Expression

Mammalian

Mutation

contain only aa1-480

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only
GFP-GW1D1b(aa502-751)

Plasmid

#21521

Depositor

Edward Chan

Publication

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

TNRC6A (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contain aa502-751

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
NHA-PIWI(aa478-860)

Plasmid

#21541

Depositor

Edward Chan

Publication

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Tags

NHA

Expression

Mammalian

Mutation

contain only aa478-860

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
pDisplay-GRAB-rACh1l-IRES-EGFP-CAAX

Plasmid

#234418

Purpose

Expresses the red ACh sensor GRAB rACh1l and a membrane-localized EGFP in mammalian cells

Depositor

Yulong Li

Publication

Xie et al Nat Neurosci. 2026 Jun 16. doi: 10.1038/

Insert

GRAB-rACh1l

Expression

Mammalian

Promoter

CMV

Available since

June 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn1-iCre-P2A-mRuby3

Plasmid

#235248

Purpose

AAV-mediated expression of iCre and mRuby3 in mammalian cells

Depositor

Takeshi Imai

Publication

Egashira et al Sci Adv. 2026 Jan 16;12(3):eadw8458. doi

Inserts

iCre
mRuby3

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hsyn1

Available since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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UAS:syp-dL5-mNeonGreen

Plasmid

#241798

Purpose

Expresses synaptically localized dL5 protein fused to mNeonGreen, under control of UAS promoter

Depositor

Harold Burgess

Publication

Sharma et al bioRxiv [Preprint]. 2025 Apr 5:2025.04.0

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

sypb (sypb Zebrafish)
dL5
mNeonGreen (zebrafish codon optimized)

Use

Zebrafish transgenesis (tol1)

Available since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-48xCCAT1 array

Plasmid

#236369

Purpose

Expression of array of 48 crRNAs targeting CCAT1

Depositor

Ling-Ling Chen

Publication

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

48xCCAT1 crRNA array (CCAT1 Human)

Use

CRISPR and Lentiviral

Available since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pB-2x-lns-DEST (JDW 1206)

Plasmid

#229818

Purpose

A PiggyBac destination vector compatible with gateway with 2 core cHS4 insulators by each ITR site

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-stmn1b-KALTA4

Plasmid

#239987

Purpose

Expresses GAL4 under control of the stmn1b promoter

Depositor

Summer Thyme

Publication

Moyer et al bioRxiv [Preprint]. 2025 Mar 5:2025.02.2

Inserts

KALTA4
EGFP

Use

Zebrafish tol2/phic31 compatible

Promoter

myl7 and stmn1b

Available since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV Ef1a DIO GeNL_SS hGtACR1

Plasmid

#233511

Purpose

Luciferase opsin fusion protein (luminopsin) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Publication

Ikefuama et al iScience. 2025 Feb 13;28(3):112022. doi:

Inserts

mNeonGreen
ISN-IE
hGtACR1

Use

AAV

Tags

Gaussia luciferase signal sequence

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_Helper

Plasmid

#231420

Purpose

M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ296.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

gp1-gp11

Use

Synthetic Biology

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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VT028327-FRT10-STOP-FRT10-GAL4

Plasmid

#232829

Purpose

Enhancer VT028327 driven GAL4, gated by Sparse.FRT10

Depositor

Liqun Luo

Publication

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MCP/PCP red RNA Lantern

Plasmid

#226097

Purpose

RNA tracking

Depositor

Jennifer Prescher

Publication

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-mScarlet-I-LgBiT-FLAG

Expression

Mammalian

Promoter

CMV

Available since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pEMS2387

Plasmid

#225633

Purpose

AAV plasmid with Ple389 (ADORA2A MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Publication

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

Ple389 (ADORA2A Human)

Use

AAV

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)

Plasmid

#224532

Purpose

A Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyA

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-H2A-mCherry-SV40pA

Use

Gateway Cloning

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMX-Sox17-p2A-Erg-t2A-GFP

Plasmid

#222496

Purpose

mouse fibroblast-to-endothelial cell direct reprogramming

Depositor

Li Qian

Publication

Farber et al Nat Commun. 2024 May 16;15(1):4170. doi:

Inserts

Sox17 (Sox17 Mouse)
Erg (Erg )
EGFP

Use

Retroviral

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAG426-GAL-UBQLN2

Plasmid

#181708

Purpose

Galactose inducible expression of UBQLN2

Depositor

Alejandro Chavez

Insert

UBQLN2 (UBQLN2 Human)

Expression

Yeast

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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Fluorescent reporter for MOBE1-2

Plasmid

#219945

Purpose

Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2

Depositor

Alexis Komor

Publication

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end

Use

CRISPR

Expression

Mammalian

Mutation

EGFP(A111V/L202S)

Promoter

CMV, U6

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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