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pCFD2-dU6:2gRNA

Plasmid

#49409

Purpose

expression of gRNA under control of the Drosophila U6:2 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-2:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-2

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pJET1.2-STOP-dsRed

Plasmid

#60944

Purpose

Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

dsRED

Use

Unspecified

Promoter

3XP3

Available Since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-VCre

Plasmid

#89575

Purpose

Expresses VCre under pCAG

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

VCre recombinase

Use

Cre/Lox and Synthetic Biology

Tags

NLS

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pX330S-5

Plasmid

#58781

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_MeltCasp1-37-GFP

Plasmid

#234069

Purpose

Encodes the caspase1 without CARD domain (with R391K mutation) controlled by a Melt variant with a switching temperature of 37°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltCasp1-37-GFP

Use

Lentiviral

Expression

Mammalian

Mutation

Melt(C292A,Q355N); hcaspase1(R391K)

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-1-AtUbi10-6

Plasmid

#197700

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AtUbi10 promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCBI-CRY2PHR-VPR

Plasmid

#192606

Purpose

Plasmid for constitutive expression of CRY2PHR-VPR.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

CRY2PHR-VPR

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFH2.156_LV-Zeo

Plasmid

#205554

Purpose

Express zeocin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Zeo

Use

Lentiviral

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-GFPuv

Plasmid

#127078

Purpose

Low phosphate inducible yibD gene promoter expressing GFPuv reporter

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

GFPuv

Expression

Bacterial

Promoter

yibDp

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pV1200

Plasmid

#111429

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)

Depositor

Gerald Fink

Article

Vyas et al Sci Adv. 2015;1(3):e1500248.

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGP-FB-orig BA

Plasmid

#13420

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

orig BA

Tags

Gal11P

Expression

Bacterial

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)

Plasmid

#70745

Purpose

for building 3-color reporter plasmid

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

mCherry-ssrA

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA-myc-HisA-Sirt3

Plasmid

#24924

Depositor

Toren Finkel

Article

Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

Insert

Sirt3 (SIRT3 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
CAG-mCherry-LacZshRNA

Plasmid

#73978

Purpose

LacZshRNA (mir155 backbone) was inserted into the 3'UTR of mCherry.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

shRNA that targets the LacZ gene

Expression

Mammalian

Promoter

CAG

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pTRANS_250d

Plasmid

#91124

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

PvUbi2

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET30-T7-lacO-His-sfGFP

Plasmid

#180754

Purpose

pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Mutation

G140C- Please see depositor comments

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBHM2662

Plasmid

#234501

Purpose

Linker-Cno_mNeonGreen-CBP-2xFLAG-CnU6-sgRNA-amdS

Depositor

Hiten Madhani

Article

Nalley et al G3 (Bethesda). 2025 Aug 6;15(8):jkaf118.

Insert

mNeonGreen-CBP-2XFLAG-amdS-PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-1-ZmPLTP-6

Plasmid

#197713

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmPLTP promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-9-35S-11

Plasmid

#197759

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

35S terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-HiBiTGFPHis

Plasmid

#188317

Purpose

Expression of HiBiT-GFP via T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

HiBiT-GFP fusion

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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