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Showing: 2501 - 2550 of 6247 results

  3. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

    Plasmid
    #35498
    Purpose
    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.
    Depositor
    Karl Deisseroth
    Article
    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    Ef1a
    Available since
    April 18, 2012
    Availability
    Academic Institutions and Nonprofits only
    35498-plasmid-map-sequence-id-199355

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  13. pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50470
    Purpose
    Gq-coupled hM3D-IRES-mCitrine under the control of GFAP promoter
    Depositor
    Bryan Roth
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    GFAP
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50470-plasmid-map-sequence-id-75489

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  14. pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50463
    Purpose
    Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50463-plasmid-map-sequence-id-75416

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  20. pattB-LSL-AAVR-F2A-spCas9

    Plasmid
    #202459
    Purpose
    The plasmid backbone used to generate the recombination template to generate the SELECTIV mice through Integrase Mediated Transgenesis. Allows for Cre-dependent expression of AAVR and Cas9.
    Depositor
    Jan Carette
    Article
    Zengel et al Nat Methods. 2023 Jul;20(7):1070-1081. d
    Insert
    AAVR (AU040320 Mouse)
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    mCherry fusion
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    202459-plasmid-map-sequence-id-402992

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  24. pAAV-CMV-FLEX-SaCas9-U6-sgNts

    Plasmid
    #159909
    Purpose
    Mutagenesis of Nts
    Depositor
    Larry Zweifel
    Insert
    Nts (Nts Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159909-plasmid-map-sequence-id-317315

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  26. pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3

    Plasmid
    #165430
    Purpose
    Expresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.
    Depositor
    Hideji Murakoshi
    Article
    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1
    Insert
    FHS-paCaMKII(SD)
    Use
    AAV
    Tags
    FHS(Flag, HisX6, Strep) tag
    Expression
    Mammalian
    Mutation
    C450A, L514K, G528A, L531E, N538E: Light-insensit…
    Promoter
    CaMKII0.4
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    165430-plasmid-map-sequence-id-325945

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  27. pAAV-hDlx-Flex-GFP-Fishell_6

    Plasmid
    #83895
    Purpose
    Cre recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV8, and AAV9
    Insert
    eGFP
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hDlx
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    83895-plasmid-map-sequence-id-160320

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  34. pAAV-CaMKIIa-eNpHR 3.0-EYFP

    Plasmid
    #26971
    Purpose
    AAV expression of eNpHR 3.0 fused to EYFP driven by CaMKIIa promoter for optogenetic inhibition
    Depositor
    Karl Deisseroth
    Has Service
    AAV1 and AAV9
    Insert
    eNpHR 3.0
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Trafficking Signal (TS) ER Export Signal
    Promoter
    CaMKIIa
    Available since
    Feb. 4, 2011
    Availability
    Academic Institutions and Nonprofits only
    26971-plasmid-map-sequence-id-196840

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  35. pAAV-TRE-DIO-Casp3-TEV

    Plasmid
    #183766
    Purpose
    This plasmid is for use with neuronal cell-type selective activity tagging. The Caspase3 expression requires both neural activity and Cre recombinase.
    Depositor
    William Wisden
    Article
    Yu et al Science. 2022 Jul;377(6601):63-72. doi:
    Insert
    AAV transgene - tetO promoter-flex/DIO-Caspase3-TEV
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO promoter
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183766-plasmid-map-sequence-id-361683

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  38. pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

    Plasmid
    #159903
    Purpose
    Mutagenesis of Oprk1
    Depositor
    Larry Zweifel
    Insert
    Oprk1 (Oprk1 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159903-plasmid-map-sequence-id-317307

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  45. pOTTC1740 - pAAV SYN1 DIO HA-hM4D(Gi)

    Plasmid
    #125146
    Purpose
    An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter
    Depositor
    Christopher Richie
    Insert
    HA-tagged hM4D(Gi) (CHRM4 Human)
    Use
    AAV and Cre/Lox
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    SYN1
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125146-plasmid-map-sequence-id-244357

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Showing: 2501 - 2550 of 6247 results