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Plasmid#217584PurposeDestination vector with kanamycin resistance and p15A origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only
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DVA_p15A_AG
Plasmid#217603PurposeDestination vector with ampicillin resistance and p15A origin of replication. Carries A-G CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVA_p15A_EH
Plasmid#217605PurposeDestination vector with ampicillin resistance and p15A origin of replication. Carries E-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVA_pBR322_AG
Plasmid#217607PurposeDestination vector with ampicillin resistance and pBR322 origin of replication. Carries A-G CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_AF
Plasmid#217598PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_EF
Plasmid#217599PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_FG
Plasmid#217600PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_GH
Plasmid#217601PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_pBR322_AE
Plasmid#217597PurposeDestination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_p15A_AF
Plasmid#217588PurposeDestination vector with chloramphenicol resistance and p15A origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVC_p15A_GH
Plasmid#217591PurposeDestination vector with chloramphenicol resistance and p15A origin of replication. Carries G-H CIDAR MoClo overhangs for level 1 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVA_pBR322_AF
Plasmid#217608PurposeDestination vector with ampicillin resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
DVA_pBR322_EH
Plasmid#217609PurposeDestination vector with ampicillin resistance and pBR322 origin of replication. Carries E-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationNonePromoterAvailable sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-V3-FETCH1
Plasmid#210650PurposeIn vitro expression of V3-FETCH reporter from the T7 promoterDepositorInsertV3-FETCH1
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-FETCH2
Plasmid#210645PurposeIn vitro expression of FETCH reporter from the T7 promoterDepositorInsertFETCH2
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sFLAG-FETCH1
Plasmid#210646PurposeIn vitro expression of FETCH reporter from the T7 promoterDepositorInsertFETCH1
UseSynthetic BiologyTagssuperFLAG (DYKDEDLL)ExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sFLAG-FETCH2
Plasmid#210647PurposeIn vitro expression of FETCH reporter from the T7 promoterDepositorInsertFETCH2
UseSynthetic BiologyTagssuperFLAG (DYKDEDLL)ExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-FETCH2-StrepII
Plasmid#210649PurposeIn vitro expression of FETCH reporter from the T7 promoterDepositorInsertFETCH2
UseSynthetic BiologyTagsStrep-TagIIExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM54
Plasmid#220830PurposeEryA sensor with increased sensitivity due to phosphorylation of EryA by mphA, which traps EryA in cellDepositorInsertmphA
UseTagsExpressionMutationPromoterPmphAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCM98
Plasmid#220831PurposeEryA sensor with further increased sensitivity by mutation of mphR (T17R), in addition to mphA phosphorylationDepositorInsertmphR
UseTagsExpressionMutationT17RPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only