Addgene

We narrowed to 1,213 results for: SUMO

Showing: 1001 - 1020 of 1213 results

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pGP-CAG-iGABASnFR2-WPRE-bGH-polyA

Plasmid

#218868

Purpose

Mammalian expression of improved GABA sensor (positive change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2

Use

Tags

Expression

Mammalian

Mutation

S99A F102Y F104Y L178S

Promoter

CAG

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-iGABASnFR2n-WPRE

Plasmid

#218875

Purpose

AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2n

Use

AAV

Tags

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

Synapsin

Available since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA2-CAND1

Plasmid

#20719

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)

Use

Tags

HA2

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2009

Availability

Academic Institutions and Nonprofits only
pGP-AAV-CAG-iGABASnFR2n-WPRE

Plasmid

#218884

Purpose

AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2n

Use

AAV

Tags

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

CAG

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pHS1184

Plasmid

#205965

Purpose

NZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH

Use

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 SENP6

Plasmid

#14583

Depositor

Mary Dasso

Article

Mukhopadhyay et al J Cell Biol. 2006 Sep 25. 174(7):939-49.

Insert

SENP6 (SENP6 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2007

Availability

Academic Institutions and Nonprofits only
pGEX-KG-CAND1

Plasmid

#19949

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pW211-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR

Plasmid

#170809

Purpose

Lentiviral vector to co-express an spsgRNA with NLS-mNeonGreen

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#170810

Purpose

Lentiviral vector to co-express an spsgRNA with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMALp2x-CARP-1

Plasmid

#41727

Depositor

Koji Okamoto

Article

Nakano et al Proc Natl Acad Sci U S A. 2006 Sep 12;10

Insert

clam ADP-ribosylating protein-1

Use

Bacterial subcloning

Tags

Expression

Mutation

deleted 5'-UTR nucleotides 1-64

Promoter

pMB1

Available since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
pGP-AAV-GFAP-iGABASnFR2n-WPRE

Plasmid

#218872

Purpose

AAV-mediated expression of improved GABA sensor (negative change in fluorescence)

Depositor

GENIE Project

Article

Kolb et al bioRxiv 2025.03.25.644953

Insert

iGABASnFR2n

Use

AAV

Tags

Expression

Mammalian

Mutation

S99A F104H R168P

Promoter

GFAP

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-PTEN

Plasmid

#50519

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

Insert

PTEN (PTEN Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-myc3-CUL4A delta N52

Plasmid

#19952

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CUL4A (cullin 4A) (CUL4A Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Missing 52 residues from N terminal CUL4A.

Promoter

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pDONR221-rap1-K9R

Plasmid

#84527

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Tags

Expression

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

none

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pGFP-PTEN D92A/C124A

Plasmid

#50522

Depositor

Kenneth Yamada

Article

Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

Insert

PTEN (PTEN Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

D92A/C124A

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-myc3-CUL4A delta N100

Plasmid

#19953

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

CUL4A (cullin 4A) (CUL4A Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

The longest published clone (Q13619)

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pCDNA CrkII Y221F

Plasmid

#50732

Purpose

mammalian expression of CrkII Y221F mutant

Depositor

Kenneth Yamada

Article

Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

Insert

CrkII Y221F (CRK Human)

Use

Tags

Expression

Mammalian

Mutation

Y221F

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pMS1088

Plasmid

#80159

Purpose

pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme

Depositor

Marcelo Sousa

Article

Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/

Insert

HisSUMO-Cys-4xNuG2-Ald

Use

Tags

HisSUMO

Expression

Mutation

Promoter

T7

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pW213-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR

Plasmid

#170811

Purpose

Lentiviral vector to co-express an sasgRNA with NLS-mNeonGreen

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-RAP1

Plasmid

#84525

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Tags

Expression

Mutation

no stop codon

Promoter

none

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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