We narrowed to 2,816 results for: cdna expression plasmid
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Plasmid#127313PurposeExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from CUG start codonDepositorInsertnanoLuciferase
Tags3xFLAG and PESTExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/GGG-stop-nLuc-3XFLAG
Plasmid#127311PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from negative control GGG start codon, but translation terminated immediately by stop codonDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AAA-stop-nLuc-3XFLAG
Plasmid#127310PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from negative control AAA start codon, but translation terminated immediately by stop codonDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-C-6His-mEsrra
Plasmid#173152Purposemouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cellsDepositorInsertORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)
TagsHisExpressionMammalianMutationWild typeAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MYO10-HMM-Nanotrap
Plasmid#87255PurposeExpression plasmid to perform NanoSPD 2.0 assays in mammalian cells.DepositorInsertMYO10 (Myo10 Mouse)
TagsGFP-nanotrapExpressionMammalianMutationMyo10 is truncated to contain aa.1-941PromoterCMVAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/TO-myc-BirA*-cCE
Plasmid#112713PurposeFor tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.DepositorInsertRngtt (Rngtt Mouse)
TagsBirA* (BirA R118G), HIV Rev nuclear localization …ExpressionMammalianMutationSilent mutation at Arg 132 (CGT to CGC), deleted …PromoterCMVAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-LaG16-APT1
Plasmid#234423PurposeExpress APT1 with the anti-GFP nanobody LaG16 fused at the N terminusDepositorAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-LaG16-APT2
Plasmid#234422PurposeExpress APT2 with the anti-GFP nanobody LaG16 fused at the N terminusDepositorAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-APT2-LaG16
Plasmid#234421PurposeExpress APT2 with the anti-GFP nanobody LaG16 fused at the C terminus separated by a flexible linkerDepositorAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-APT1-LaG16
Plasmid#234420PurposeExpress APT1 with the anti-GFP nanobody LaG16 fused at the C terminus separated by a flexible linkerDepositorAvailable SinceApril 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EFNB2-RBD-Fc (D62Q)
Plasmid#200979PurposeMammalian expression plasmid for soluble EFNB2 (D62Q specificity mutant) fused to IgG1 FcDepositorInsertEFNB2 (EFNB2 Human)
TagsIgG1 FcExpressionMammalianMutationSoluble Receptor-Binding Domain; D62Q mutation fo…PromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
RGS-6xHis-LIN-29-pcDNA3.1-
Plasmid#52515Purposeexpresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminusDepositorInsertLin-29 b (lin-29 Nematode)
TagsRGS-6xHisExpressionMammalianMutationwt (changed coding sequence in 5' (5th codon…PromoterCMVAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαsL-260-NLuc
Plasmid#207158PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαsS-246-Nluc
Plasmid#207157PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphasS-RLuc8
Plasmid#140980PurposeEncodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphasS-RLuc8
UseLuciferaseExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RasAR(R89L)-NES
Plasmid#205568PurposeRasAR negative-control mutant plasmid. Targeted to cytosol.DepositorInsertRasAR(R89L)-NES (RAF1 Human, Synthetic)
TagsNuclear export signal (NES)ExpressionMammalianMutationArg 89 mutated to Leu in Raf1 RBD region.PromoterCMVAvailable SinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 IRF7(5’UTR)-FF
Plasmid#85490PurposeFirefly luciferase under the control of IRF7 5'UTRDepositorAvailable SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 NDUFS6(5’UTR)-FF
Plasmid#85488PurposeFirefly luciferase under the control of NDUFS6 5'UTRDepositorAvailable SinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 T21R-vhhGFP4-T2A-mCherry
Plasmid#225587PurposeMammalian expression vector for TRIM21 RING-vhhGFP4 anti-GFP degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-vhhGFP4-T2A-mCherry
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 T21R-Nb139-T2A-mCherry
Plasmid#225589PurposeMammalian expression vector for TRIM21 RING-Nb139 anti-p53 degrader with a self-cleaving T2A-mCherry fluorescent expression reporterDepositorInsertT21R-Nb139-T2A-mCherry
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only