We narrowed to 1,270 results for: grna cloning vector

1. pCB-2

   Plasmid

   #210388

   Purpose

   All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

   Depositor

   Gilles van Wezel

   Article

   Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

   Inserts

   dCas9
   CymR
   sgRNA cassette
   

   Use

   CRISPR; Integrative vector for use in streptomyces

   Mutation

   BsaI-flanked RFP cassette inserted in place of sp…

   Promoter

   SP11 and SP30

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDYT001

   Plasmid

   #186549

   Purpose

   Lineage tracing vector (with barcoded Target Sites and triple sgRNAs)

   Depositor

   Jonathan Weissman

   Article

   Yang et al Cell. 2022 Apr 28. pii: S0092-8674(22)00

   Insert

   14-bp random integration barcode and three target sites and 3x sgRNAs
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PL-5LTR-GW-A

   Plasmid

   #138480

   Purpose

   For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
   

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-2G-CNCB_sgRBBP6-68

   Plasmid

   #237555

   Purpose

   A piggybac-based vector containing mouse U6 promoter-driven RBBP6 sgRNA #6, human U6 promoter-driven RBBP6 sgRNA #8 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

   Depositor

   Kazutoshi Takahashi

   Article

   Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

   Insert

   RBBP6 (RBBP6 Human)
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   mouse U6 promoter and mouse U6 promoter, human U6…

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2X_pX458_pSpCas9(BB)-2A-GFP_eSOX17.2_KO

   Plasmid

   #195495

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the core endoderm distal enhancer of human SOX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA (SOX17 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDGE503

   Plasmid

   #153276

   Purpose

   Intermediate sgRNA array cloning vector position 1

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGE504

   Plasmid

   #153277

   Purpose

   Intermediate sgRNA array cloning vector position 2

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGE506

   Plasmid

   #153279

   Purpose

   Intermediate sgRNA array cloning vector position 4

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGE505

   Plasmid

   #153278

   Purpose

   Intermediate sgRNA array cloning vector position 3

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX335_U6-Chimeric_BB-CBh-hSpCas9n(D10A)_SOX17_Ex2_KI

    Plasmid

    #195502

    Purpose

    Cas9 nickase expression vector bearing a sgRNA targeting Exon 2 of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA (SOX17 Human)
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGE508

    Plasmid

    #153280

    Purpose

    End-linker for cloning 2 sgRNA arrays into any recipient vector

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    linker
    

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. c3GIC9

    Plasmid

    #62191

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3x FLAG

    Promoter

    TRE3G

    Available Since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDGE509

    Plasmid

    #153281

    Purpose

    End-linker for cloning 3 sgRNA arrays into any recipient vector

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    linker
    

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGE510

    Plasmid

    #153282

    Purpose

    End-linker for cloning 4 sgRNA arrays into any recipient vector

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    linker
    

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-U6-CNCB

    Plasmid

    #236763

    Purpose

    A piggybac-based cloning vector containing mouse U6 promoter-driven sgRNA for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.

    Depositor

    Kazutoshi Takahashi

    Article

    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

    Type

    Empty backbone

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    mouse U6 promoter, CAG promoter

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1

    Plasmid

    #187925

    Purpose

    sgRNA vector backbone expressing puromycin resistance and CD90.1 as selection markers

    Depositor

    Martin Turner

    Article

    Turner et al eLife. 2022 Apr 22;11. pii: 72313. doi:

    Insert

    hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-CD90.1
    

    Use

    CRISPR and Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-UCOE-SFFV-dCas9-P2A-EGFP

    Plasmid

    #188898

    Purpose

    Human lentiviral vector for expression of dCas9 with a C-terminal HA-2xNLS and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and P2A-GFP

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPC1000

    Plasmid

    #174829

    Purpose

    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Inserts

    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    EF1a and mouse U6

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. MultiMate-HITI-2c ACTB

    Plasmid

    #206267

    Purpose

    All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
    

    Use

    CRISPR; Recombinant baculovirus production

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEIF3D-WT-sgNT

    Plasmid

    #222120

    Purpose

    EIF3D overexpression vector with non-targeting sgRNA

    Depositor

    Jonathan Weissman

    Article

    She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

    Insert

    eIF3D (EIF3D Human)
    

    Use

    Lentiviral

    Tags

    BFP-P2A

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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