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We narrowed to 1,273 results for: grna cloning vector

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Showing: 1001 - 1020 of 1273 results

  1. PB-2G-CNCB_sgRBBP6-68

    Plasmid
    #237555
    Purpose
    A piggybac-based vector containing mouse U6 promoter-driven RBBP6 sgRNA #6, human U6 promoter-driven RBBP6 sgRNA #8 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Insert
    RBBP6 (RBBP6 Human)
    Use
    Piggybac
    Expression
    Mammalian
    Promoter
    mouse U6 promoter and mouse U6 promoter, human U6…
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    237555-plasmid-map-sequence-id-473543

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  9. c3GIC9

    Plasmid
    #62191
    Purpose
    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes
    Depositor
    Lukas Dow
    Article
    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do
    Insert
    humanized S. Pyogenes Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Promoter
    TRE3G
    Available Since
    Aug. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    62191-plasmid-map-sequence-id-156341

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  14. PB-U6-CNCB

    Plasmid
    #236763
    Purpose
    A piggybac-based cloning vector containing mouse U6 promoter-driven sgRNA for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Type
    Empty backbone
    Use
    Piggybac
    Expression
    Mammalian
    Promoter
    mouse U6 promoter, CAG promoter
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    236763-plasmid-map-sequence-id-473487

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  15. LentiRCas9-Lambda2

    Plasmid
    #104184
    Purpose
    Lentiviral transfer vector that carries U6-driven non-targeting sgRNA using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)
    Depositor
    Eugene Yeo
    Article
    Batra et al Cell. 2017 Aug 24;170(5):899-912.e10. do
    Insert
    U6-sgRNA, EFS-PIN-dCas9
    Use
    CRISPR and Lentiviral
    Available Since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    104184-plasmid-map-sequence-id-201745

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  18. pPC1000

    Plasmid
    #174829
    Purpose
    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Inserts
    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    EF1a and mouse U6
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174829-plasmid-map-sequence-id-343648

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  19. MultiMate-HITI-2c ACTB

    Plasmid
    #206267
    Purpose
    All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.
    Depositor
    Imre Berger
    Article
    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP
    Use
    CRISPR; Recombinant baculovirus production
    Expression
    Mammalian
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    206267-plasmid-map-sequence-id-406623

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  20. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

    Plasmid
    #64217
    Purpose
    Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A
    Depositor
    Ralf Kuehn
    Article
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Inserts
    Cas9
    hU6 promoter; BbsI sites for sgRNA
    H1 promoter; BamHI site for shRNA
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Promoter
    CBh, H1, and U6
    Available Since
    Sept. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    64217-plasmid-map-sequence-id-123057

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Showing: 1001 - 1020 of 1273 results