We narrowed to 5,817 results for: pcas

1. lenti-SpCas9 puro

   Plasmid

   #104994

   Purpose

   This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-ChroME2f-mRuby2-ST

   Plasmid

   #170160

   Purpose

   Cation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2f-mRuby2-ST
   

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG mEmerald-EB3

   Plasmid

   #168514

   Purpose

   Expression of human plus-TIP microtubule binding protein EB3 fused with mEmerald for microtubule +end live tracking

   Depositor

   Franck Polleux

   Article

   Lewis TL et al Curr Biol. 2016 Oct 10;26(19):2602-2608.

   Insert

   EB3 (MAPRE3 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG mito-GCaMP5G

   Plasmid

   #105009

   Purpose

   mitochondrial calcium sensor

   Depositor

   Franck Polleux

   Article

   Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

   Insert

   GCaMP5G
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG mito-mEos2

   Plasmid

   #168515

   Purpose

   Expression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rate

   Depositor

   Franck Polleux

   Article

   Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

   Insert

   mito-mEos2
   

   Tags

   mEos2

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-48xH3C1 array

   Plasmid

   #236384

   Purpose

   Expression of array of 48 crRNAs targeting H3C1

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   48xH3C1 crRNA array (H3C1 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAX APP delta CT

   Plasmid

   #30143

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein (APP Human)
   

   Expression

   Mammalian

   Mutation

   deletion of C-terminal intracellular domain (afte…

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pCAG-G-Flamp1-mut

   Plasmid

   #188568

   Purpose

   control probe of G-Flamp1, insensitive to cAMP

   Depositor

   Jun Chu

   Article

   Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

   Insert

   G-Flamp1-mut
   

   Tags

   His tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9n(BB)-2A-Puro (PX462)

   Plasmid

   #48141

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pCAG-WHaloCaMP1a-EGFP

    Plasmid

    #205313

    Purpose

    Expression of WHaloCaMP1a-EGFP in mammalian cells from the CAG promoter

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a-EGFP
    

    Expression

    Mammalian

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9 TadCBEd-V106W

    Plasmid

    #193839

    Purpose

    Express TadCBEd-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDd V106W-SpCas9 D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-FuG-E (P440E)

    Plasmid

    #119978

    Purpose

    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E (P440E) envelope coating virus particle with NeuRet.

    Depositor

    Kazuto Kobayashi

    Article

    Kato et al J Neurosci Methods. 2018 Oct 19;311:147-

    Insert

    Fusion Glycoprotein type E (P440E)
    

    Use

    Lentiviral

    Tags

    Fusion protein of Vesicular stomatitis Indiana vi…

    Promoter

    CAGGS

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-Puro-C9orf72

    Plasmid

    #83439

    Purpose

    Expresses Cas9 and a gRNA targeting C9orf72

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    gRNA targeting human C9orf72 (C9orf72 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-GluA2-Fab-LC

    Plasmid

    #230063

    Purpose

    Expresses light chain Fab fragment of anti-GluA2. Can be used with heavy chain to produce Fab in HEK cells.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA2 Fab light chain fragment
    

    Expression

    Mammalian

    Promoter

    Chicken beta actin (CAG)

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR_TRE_mcherry-P2A-Pcad

    Plasmid

    #133807

    Purpose

    inducible expression of mCherry and Pcad in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2018 Jul 13;361(6398):156-162.

    Insert

    cadherin 3 (CDH3)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG cyto-RCaMP1h

    Plasmid

    #105014

    Purpose

    cytosolic calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    RCaMP1h
    

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. NM9-SpCas9-NLS3

    Plasmid

    #128177

    Purpose

    Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

    Depositor

    Huimin Zhao

    Article

    Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

    Insert

    SpCas9-NLS3
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    pPGK1

    Available Since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-Flag-hsDicer

    Plasmid

    #41584

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-MCS-EGFP

    Plasmid

    #164092

    Purpose

    expression of EGFP-tagged proteins under CAG promoter

    Depositor

    Maik Hintze

    Insert

    EGFP
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. G1333 DddAtox-C–dSpCas9

    Plasmid

    #157834

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
    

    Expression

    Mammalian

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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