Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 2,376 results for: pet28

Clear keywords
Showing: 1001 - 1020 of 2376 results

  1. pET28a-FgßF3-ddFLN4-HIS-ybbr

    Plasmid
    #101743
    Purpose
    mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    FgßF3 peptide, ddFLN4 fingerprint (FGB Dictyostelium discoideum, Human)
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4, added 9F in FgßF3
    Promoter
    T7
    Available Since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101743-plasmid-map-sequence-id-199091

    Enlarge

  2. delNC C-His Ana GvpC in pET28a

    Plasmid
    #85736
    Purpose
    Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi
    Insert
    Gas vesicle protein C (truncated and his-tagged)
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Deletion of N and C terminal regions, addition of…
    Promoter
    T7
    Available Since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    85736-plasmid-map-sequence-id-174840

    Enlarge

  6. pET28b(+)_S. cerevisiae Hbs1(166-611)

    Plasmid
    #39338
    Depositor
    SeWon Suh
    Insert
    Elongation factor 1 alpha-like protein
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 17, 2012
    Availability
    Academic Institutions and Nonprofits only

  8. pET28a(+)v2_TwinStrep-SUMO-FLP-8xHis

    Plasmid
    #246980
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged FLP recombinase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    FLP Recombinase
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    246980-plasmid-map-sequence-id-491526

    Enlarge

  10. pET28b-SUMO-FLAG-TeKaiA-FLAG

    Plasmid
    #229220
    Purpose
    For expression of T. elongatus KaiA protein
    Depositor
    Andy LiWang
    Insert
    kaiA (kaiA T. elongatus)
    Tags
    FLAG and SUMO-FLAG
    Expression
    Bacterial
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229220-plasmid-map-sequence-id-460016

    Enlarge

  11. pET28b-SUMO-FLAG-TeKaiB-FLAG

    Plasmid
    #229221
    Purpose
    For expression of T. elongatus KaiB protein
    Depositor
    Andy LiWang
    Insert
    kaiB (kaiB T. elongatus)
    Tags
    FLAG and SUMO-FLAG
    Expression
    Bacterial
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229221-plasmid-map-sequence-id-457227

    Enlarge

  12. pET28b-SUMO-SeKaiB-K25C-FLAG

    Plasmid
    #229225
    Purpose
    For expression of S. elongatus KaiB protein mutant
    Depositor
    Andy LiWang
    Insert
    kaiB (kaiB S. elongatus)
    Tags
    FLAG and SUMO
    Expression
    Bacterial
    Mutation
    K25C
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    229225-plasmid-map-sequence-id-457071

    Enlarge

  16. pET28a-SdrG-B2-ELP-HIS-ybbR

    Plasmid
    #169134
    Purpose
    E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.
    Depositor
    Michael Nash
    Article
    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi
    Insert
    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    Tags
    3xELP linker, 6x Histag, and ybbr tag
    Expression
    Bacterial
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    169134-plasmid-map-sequence-id-380322

    Enlarge

  19. pET28a-SdrG-B1-ELP-HIS-ybbR

    Plasmid
    #169133
    Purpose
    E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.
    Depositor
    Michael Nash
    Article
    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi
    Insert
    Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
    Tags
    3xELP linker, 6x Histag, and ybbr tag
    Expression
    Bacterial
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    169133-plasmid-map-sequence-id-361153

    Enlarge

  20. pET28_6xHis-GAL4-PARP1-CAT-L713F

    Plasmid
    #175949
    Purpose
    Bacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutation
    Depositor
    Joan Conaway
    Article
    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118
    Insert
    GAL4 DNA binding domain (GAL4 Budding Yeast)
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    L713F, V762A for the 'PARP1-CAT-L713F' …
    Promoter
    T7
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    175949-plasmid-map-sequence-id-345352

    Enlarge

Showing: 1001 - 1020 of 2376 results