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Plasmid#67108PurposeStandard for cell-free expression benchmarking.DepositorInsertGTF2H1 (GTF2H1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro
Plasmid#71236PurposeExpress sgRNA and dCas9-KRAB from lentiviral vectorDepositorInsertshumanized dCas9-KRAB T2A Puro
sgRNA
UseCRISPR and LentiviralTagsFlagExpressionMutationD10A and H840APromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCrysβ:ECFP-LexOP:mCherry-KrasG12V
Plasmid#89888PurposeHuman KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.DepositorInsertmCherry-KRasG12V
UseUnspecifiedTagsmCherryExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mClover3-KASH5-DN
Plasmid#171492PurposeT7 promotor drives in vitro transcription of mClover3-tagged bovine Kash5 DN mRNADepositorInsertKASH5-DN (CCDC155 B. tarus (bovine))
UsePgehmeTagsmClover3ExpressionMutationPromoterT7Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-GST-KASH5-DN
Plasmid#171493PurposeT7 promotor drives in vitro transcription of GST-tagged bovine Kash5 DN mRNADepositorInsertKASH5-DN (CCDC155 B. tarus (bovine))
UsePgehmeTagsGSTExpressionMutationPromoterT7Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,
Plasmid#110201PurposeMelanocyte-specific expression of the LexPR-2A-Cerulean fusionDepositorInsertLexPR transactivator - 2A - Cerulean
UseTagsCeruleanExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-tat:msfGFP
Plasmid#194915PurposeTetracycline inducible expression of msfGFP fused to Tat signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Tat signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-sec:msfGFP
Plasmid#194914PurposeTetracycline inducible expression of msfGFP fused to Sec signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-HA
Plasmid#61355Purposeencodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal HA tag
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCrysβ:ECFP-LexOP:mCherry-NRasQ61K
Plasmid#89886PurposeHuman NRasQ61K fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.DepositorInsertmCherry-NRasQ61K
UseUnspecifiedTagsmCherryExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCrysβ:ECFP-LexOP:mCherry-HrasG12V
Plasmid#89887PurposeHuman HRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.DepositorInsertmCherry-HRasG12V
UseUnspecifiedTagsmCherryExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
PVRL2-bio-His
Plasmid#51824PurposeExpresses full-length Poliovirus receptor-related protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPVRL2 (NECTIN2 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pD21-hgAPOAI
Plasmid#124962PurposeExpress ApoAI in the liverDepositorInserthuman APOAI gene (LPA Human)
UseAdenoviralTagsExpressionMutationPromoterhuman APOAIAvailable sinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-KLF3
Plasmid#49100PurposeExpresses KLF3 in mammalian cellsDepositorInsertKrüppel-Like Factor 3(KLF3)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mH33B-mClover3
Plasmid#171496PurposeT7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNADepositorInsertH3.3B (H3f3b Mouse)
UsePgehmeTagsmClover3ExpressionMutationPromoterT7Available sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-CCR5
Plasmid#222309PurposeSynchronize the trafficking of CCR5 from the ER.DepositorInsertStreptavidin-KDEL and CCR5 fused to SBP-EGFP (CCR5 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-mCherry-CCR1
Plasmid#222311PurposeSynchronize the trafficking of CCR1 from the ER.DepositorInsertStreptavidin-KDEL and CCR1 fused to SBP-mCherry (CCR1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoBb.1
Plasmid#121898PurposePlasmid for making zebrafish apoBb.1 in situ probeDepositorInsertApoBb.1 (apobb.1 Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoA-IVb.1
Plasmid#121903PurposePlasmid for making zebrafish apoA-IVb.1 in situ probeDepositorInsertApoa4b.1 (apoa4b.1 Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCRII apoA-IVb.2
Plasmid#121904PurposePlasmid for making zebrafish apoA-IVb.2 in situ probeDepositorInsertApoa4b.2 (apoa4b.2 Zebrafish)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only