We narrowed to 7,531 results for: ski

1. pB194

   Plasmid

   #61154

   Purpose

   Expression of KIN28 under GAL1 promoter in yeast

   Depositor

   Charles Brenner

   Article

   Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

   Insert

   TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   Gal1

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-alphaCaMKII-E2-Crimson-3XNLS-pA

   Plasmid

   #183803

   Purpose

   AAV vector to express nuclear localized E2-Crimson from CaMKIIa promoter

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

   Insert

   E2-Crimson-NLS
   

   Use

   AAV

   Tags

   NLS

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAPM D4 BsmBI Myd88 stuffer

   Plasmid

   #128505

   Purpose

   pAPM knockdown vector with Myd88 stuffer sequence

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   Puromycin Resistance
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCI-MEG3c

   Plasmid

   #59422

   Purpose

   Expresses lncRNA MEG3 isoform c in mammalian cells.

   Depositor

   Anne Klibanski

   Article

   Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

   Insert

   MEG3c (MEG3 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 PDGFRB

   Plasmid

   #67130

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PDGFRB (PDGFRB Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. XE101 alpha transducin CS2+

   Plasmid

   #16681

   Depositor

   Randall Moon

   Article

   Slusarski et al Nature. 1997 Nov 27;390(6658):410-3. doi

   Insert

   alpha-transducin (GNAT1 Human)
   

   Use

   Xenopus expression

   Available Since

   March 13, 2008

   Availability

   Academic Institutions and Nonprofits only

7. ptcALPS gag-gfp Blasti

   Plasmid

   #115806

   Purpose

   Express gag-gfp fusion driven by TK promoter

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   gag-gfp
   

   Use

   Lentiviral

   Promoter

   TK

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2xFRB-myc

   Plasmid

   #20227

   Depositor

   Carolyn Bertozzi

   Article

   Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

   Insert

   2xFRB (MTOR Human)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   1 FRB copy has a T2098L point mutation

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pFA6a-link-GFPγ-SpHis5

   Plasmid

   #60781

   Purpose

   Yeast C-terminal tagging vector, which creates gene fusions with GFPγ using SpHis5 selection

   Depositor

   Linda Huang

   Article

   Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

   Insert

   GFPγ
   

   Use

   Yeast integrating

   Mutation

   T65C S72A V163A I167T L231H

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCXLE-dCas9GFP-shP53

    Plasmid

    #102906

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9GFP-shP53
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCI-MEG3d

    Plasmid

    #59423

    Purpose

    Expresses lncRNA MEG3 isoform d in mammalian cells.

    Depositor

    Anne Klibanski

    Article

    Zhou et al J Biol Chem. 2007 Aug 24;282(34):24731-4

    Insert

    MEG3d (MEG3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLdSaCN

    Plasmid

    #123261

    Purpose

    Expresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

    Insert

    gRNA and SaCas9
    

    Use

    CRISPR; Leishmania

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCellFree_G03 PRKCA

    Plasmid

    #67115

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PRKCA Protein kinase C alpha type (PRKCA Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pJEP223-AAV-EFS-GFP-pA

    Plasmid

    #62913

    Purpose

    AAV vector backbone designed to express GFP from a EFS promoter. It also contains a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    AAV

    Promoter

    short form of Elongation Factor1α (EFS)

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. MAGE-A12Pro pGL4basic

    Plasmid

    #78139

    Purpose

    luciferase reporter plasmid

    Depositor

    Heidi Schwarzenbach

    Article

    Wischnewski et al Mol Cancer Res. 2007 Jul;5(7):749-59.

    Insert

    MAGE-A12 (MAGEA12 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    contains -425/+115 of promoter

    Promoter

    MAGE-A12

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Pr-deCFP-MGapt

    Plasmid

    #67740

    Purpose

    OR2-OR1-Pr promoter expressing deCFP-MG aptamer fusion

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deCFP-MGapt-T500
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    OR2-OR1-Pr

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Pr1-T7RNAP

    Plasmid

    #67738

    Purpose

    OR2-OR1-Pr1 promoter expressing T7 RNAP

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-T7RNAP-T500
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    OR2-OR1-Pr1

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCellFree_G03 PPARG

    Plasmid

    #67099

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PPARG (PPARG Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCellFree_G03 PSMC1

    Plasmid

    #67087

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    PSMC1 (PSMC1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Mutation

    E275G, I287V and R294G

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pEPOR2KN0040

    Plasmid

    #117607

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
    

    Expression

    Plant

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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