We narrowed to 3,167 results for: tef

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pDH22

Plasmid

#83798

Purpose

Useful for tagging yeast proteins with N-terminal YFP tag; G418 selection

Depositor

Eric Muller

Publication

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

YFP

Tags

YFP

Expression

Yeast

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6-luc*(-SKL)-HIS3MX6

Plasmid

#40233

Depositor

Michael Benton

Insert

luc*(-SKL)

Expression

Bacterial

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBS-SKII-3XHA-fSNAP-NAT

Plasmid

#188916

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-NAT

Expression

Yeast

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBS-SKII-3XHA-fSNAP-Hygromycin

Plasmid

#188914

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-Hygromycin

Expression

Yeast

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNS1

Plasmid

#131769

Purpose

PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Publication

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

FUSN_Fusion Red_Cry2WT

Use

Integration into δ-sites

Expression

Yeast

Promoter

PADH1

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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GAL-Aur1-2GFP-Hygromycin

Plasmid

#184775

Purpose

Yeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

proGAL-Aur1 (AUR1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pUC119- Padh81-GBP-mCherry(C)-hphMX6-lys1*

Plasmid

#89071

Purpose

genomic GBP-mCherry epitope C-terminal tagging at lys1+ locus in S. pombe

Depositor

Quanwen Jin

Publication

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Genomic integration in s. pombe

Tags

GBP-mCherry

Expression

Yeast

Available since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB3048(gRNA XII-2)

Plasmid

#73290

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-2

Depositor

Irina Borodina

Publication

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKT-mCitrineV163A-HIS

Plasmid

#64685

Purpose

pKT vector encoding yeast-codon optimized yellow fluorescent protein mCitrine V163A. The V163A mutation confers fast protein maturation.

Depositor

Erin O'Shea

Publication

Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

Insert

mCitrine V163A

Expression

Yeast

Mutation

Valine 163 changed to alanine

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFA6a-kanMX-tetO-Pcyc1-3xHA-LINKER

Plasmid

#52686

Purpose

Same as 41024, with additional linker

Depositor

Michael Nick Boddy

Insert

tetO7-Pcyc1-3xHA+LINKER

Use

Promoter replacement

Expression

Yeast

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pCY 3080-07

Plasmid

#36227

Depositor

Anne Robinson

Publication

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

Streptoalloteichus hindustanus bleomycin (Sh ble)

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEmCitrine-Zeocin

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pCSN069

Plasmid

#166728

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Publication

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-Mxi1

Use

CRISPR

Tags

Mxi1

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pG2

Plasmid

#162603

Purpose

Edit Ade2 gene in yeast

Depositor

Lars Steinmetz

Publication

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pGAL-SKI1-NAT

Plasmid

#160382

Purpose

Overxpression of SKI1/XRN1 in yeast

Depositor

John McCusker

Insert

SKI1

Expression

Yeast

Promoter

GAL

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNS3

Plasmid

#131770

Purpose

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

Depositor

Jared Toettcher

Publication

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_FUSN_Fusion Red_Cry2Olig_TACT1

Use

Integration into δ-sites

Expression

Yeast

Mutation

E490G to make Cry2 into Cry2Olig

Promoter

PADH1

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pK3F

Plasmid

#85770

Purpose

N-ICE plasmid pK3F

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCY 3010-07

Plasmid

#36212

Depositor

Anne Robinson

Publication

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

Streptoalloteichus hindustanus bleomycin (Sh ble)

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-Cerulean-Zeocin

Available since

June 19, 2012

Availability

Academic Institutions and Nonprofits only

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pGAL-flp(H305L)-NAT

Plasmid

#160380

Purpose

overexpression of Flp recombinase step-arrest mutant in yeast

Depositor

John McCusker

Insert

FLP1

Expression

Yeast

Mutation

H305L

Promoter

GAL

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pfa6a PhoCl-TsCC(B)::KANMX

Plasmid

#185906

Purpose

Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

Depositor

Matthew Good

Publication

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

PhoCl-TsCC(B)

Tags

PhoCl fluorophore with C-terminal TsCC(B)

Expression

Yeast

Promoter

SP6

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pH3FS-PYK1

Plasmid

#85781

Purpose

N-ICE plasmid pH3FS-PYK1

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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