We narrowed to 18,168 results for: JUN

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pGEX-2T-CBP(1603-2678)

Plasmid

#183774

Purpose

Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

Insert

nej (nej Fly)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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HEpl016

Plasmid

#179991

Purpose

pCBh_3xFLAG_NLS_SiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized SiCas12i driven by CBh promoter and tagmCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

SiCas12i

Tags

3xFlag

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-SNAP-CD4-bla

Plasmid

#179452

Purpose

Donor vector to knock in SNAPtag C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

SnapTag

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_840-1605-del1097-1110-RNFtoAAA_X

Plasmid

#148000

Purpose

Mammalian Expression of HsNot1iso1_840-1605-del1097-1110-RNFtoAAA

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_840-1605-del1097-1110-RNFtoAAA (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep

Plasmid

#180811

Purpose

Expression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

GST-BTLAint (AA190-289, Y257F, Y282F)-TwinStrep (BTLA Human)

Expression

Bacterial

Mutation

BTLA (Y257F, Y282F)

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His10-BTLAicd (AA190-289)-TwinStrep

Plasmid

#180805

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289)-TwinStrep (BTLA Human)

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSL008_phiC31_neo-5xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

Plasmid

#179426

Purpose

Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-5kb-mch

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMH010_phiC31_neo-5xTetO-pEF-H2B-mCitrine-1.2kb,lambda-pRSV-H2B-mCherry

Plasmid

#179427

Purpose

Dual fluorescent reporter construct with 1.2kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-1.2kb-mch

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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S+Q111R

Plasmid

#167173

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R mutation in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-S+Q111R
ATP1B1

Expression

Bacterial and Insect

Mutation

Changed Q at 111 to R

Promoter

P10 and PH

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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S+N122D

Plasmid

#167174

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with N122D mutation in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-S+N122D
ATP1B1

Expression

Bacterial and Insect

Mutation

Changed N at 111 to D

Promoter

P10 and PH

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-dre4

Plasmid

#170440

Purpose

Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

dre4 (dre4 Fly)

Tags

6xHis and V5

Expression

Bacterial

Mutation

Deleted amino acids 1-20

Promoter

T7

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00008

Plasmid

#166724

Purpose

sgRNA cloning backbone

Depositor

Raj Chari

Article

Choi et al Nat Commun. 2020 Jun 1;11(1):2718. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-nCas9

Plasmid

#163564

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045D

Plasmid

#125006

Purpose

Expresses gRNAs targeting hedgehog and wingless

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

wg gRNA (wg Fly)

Expression

Insect

Promoter

u6:3

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 295 (pDONR-Dll4-F2-ETS#2-WT-8X)

Plasmid

#156412

Purpose

Gateway middle entry clone containing an 8X multimerized ETS#2 DNA binding site from the murine Dll4 intronic enhancer

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

murine Dll4 F2- ETS site B/ site #2, 8X

Use

Gateway cloning

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-DDX43-FL-G84D

Plasmid

#134577

Purpose

Express human DDX43 mutant (full-length with G84D mutation) in E. coli

Depositor

Yuliang Wu

Article

Talwar et al J Biol Chem. 2017 Jun 23;292(25):10429-1

Insert

DDX43-G84D (DDX43 Human)

Tags

His

Expression

Bacterial

Mutation

full-length (G84D)

Promoter

T7

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCri-H6-TEV-GRAB

Plasmid

#128498

Purpose

Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)

Tags

His tag (6x)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCri-H6-TEV-GRAB-STREP

Plasmid

#128499

Purpose

Expresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

GRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)

Tags

His tag (6x) and Strep tag (2x)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Gli3_Sense

Plasmid

#124439

Purpose

Plasmid for sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

GLI-Kruppel family member GLI3 (Gli3 Mouse)

Use

In situ probe

Promoter

T7

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-K795A

Plasmid

#114061

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Jun 12;22(6):1321-9. doi:

Insert

BUBR1-K795A (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-K795A

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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