We narrowed to 57,324 results for: plasmid
-
Plasmid#129733PurposeDestination plasmid containing the 35S:XVE estradiol inducible driver and the A-G overhangs for GreenGate cloning of an expression casette for estradiole-inducible expression in plants.DepositorTypeEmpty backboneExpressionPlantAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only
-
pFastBac_His-ZZ-TEV-Lis1
Plasmid#132539PurposePlasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tagDepositorAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV8-hSyn-flex-miR30-eGFP-shDyn
Plasmid#132716PurposeEncodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Pdyn gene, which encodes dynorphinDepositorAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFnCpf1GG
Plasmid#131010PurposeBackbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.DepositorInsertsdirect repeat of FnCas12a
GFP expression cassette
UseCRISPRExpressionBacterialAvailable sinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SIM)3
Plasmid#126955PurposeBacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
TagseGFPExpressionBacterialPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMGS55 (GFP-ARF16-PB1-MCS-P2A-OsTIR1)
Plasmid#129667PurposeA repair construct to express GFP-ARF16-PB1-MCS and OsTIR1 under the control of the CMV promoter from the human AAVS1 locusDepositorInsertOsARF16-PB1 domain
TagsGFPExpressionMammalianAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP2374
Plasmid#127579PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorExpressionYeastAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CaMKIV-dCT
Plasmid#126422PurposePlasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)DepositorInsertCalcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
TagsFLAGExpressionMammalianAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHO_pTEFmut7_CggR_R250A_ble
Plasmid#124585PurposePlasmid for genomic integration of CggR_R250A repressor regulated by the pTEFmut7 promoterDepositorAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKmK.T4
Plasmid#125056PurposeStorage plasmid for K.marxianus terminator; type 4 partDepositorInsertPDC1 terminator
UseSynthetic BiologyExpressionYeastAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pREP4-Luc
Plasmid#124892PurposeThis plasmid contains EBNA sequence.DepositorPublicationInsertluc
Available sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)
Plasmid#66708Purposeexpresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removedDepositorInsertS.Pyogenes VP64-dCas9-VP64
UseLentiviral and Synthetic BiologyTagsVP64MutationD10A and H840APromoterhUbCAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG2IG-Hmga2
Plasmid#122294PurposeOverexpression for mouse Hmga2DepositorInsertHmga2 (Hmga2 Mouse)
ExpressionMammalianAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymYPET-CaURA3
Plasmid#118460PurposeYeast tagging vector to create a gene fusion with ymYPET with CaUra3 selection. *An updated version of this plasmid is available: #168056 pFA6a-link-ymYPET-URA3.DepositorInsertymYPET
ExpressionYeastMutationYPET A206K, F208S, E232L, N235DAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ytdTomato-CaURA3
Plasmid#118461PurposeYeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection. *An updated version of this plasmid is available: #168054 pFA6a-link-ytdTomato-URA3.DepositorInsertytdTomato
ExpressionYeastAvailable sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC763 - pX458 with rat Rosa26 gRNA A
Plasmid#113161PurposeA plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFPDepositorInsertgRNA for rat Rosa26
UseCRISPRExpressionMammalianPromoterhU6Available sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)
Plasmid#114086PurposeCAG promoter expression plasmid for human codon optimized LbBE1.4DepositorInserthuman codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
Tags2x SV40 NLS and SV40 NLSExpressionMammalianMutationD832APromoterCAGAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson
Plasmid#117662PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT1)-E2-Crimson
ExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1077)
Plasmid#114095PurposeCAG promoter expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized AsCas12a-RR (E174R/S542R/K607R)
TagsNLS(nucleoplasmin) and 3xHAExpressionMammalianMutationE174R, S542R and K607RAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)
Plasmid#114078PurposeCAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)DepositorInserthuman codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
TagsSV40 NLSExpressionMammalianAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by