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pTE3646

Plasmid

#137714

Purpose

Expresses LbCas12a RVRR variant in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

Insert

LbCas12a-RVRR

Tags

3xHA, NLS, and SV40 NLS

Expression

Mammalian

Mutation

G532R, K538V, Y542R, K595R

Promoter

CMV

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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FLS2-YFPn

Plasmid

#87165

Purpose

fluorescence complementation assay

Depositor

Silke Robatzek

Article

Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

Insert

FLS2 (FLS2 Mustard Weed)

Tags

YFPn

Promoter

35s

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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Flag- mCry1ER-pBABEpuro

Plasmid

#61429

Purpose

Express mCRY1-ER* fusion protein

Depositor

Aziz Sancar

Article

Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

Insert

mCry1 (Cry1 Mouse)

Use

Retroviral

Tags

Flag and mouse estrogen receptor ligand binding d…

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
MERS-coV-NSP3-c001

Plasmid

#173085

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

rep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))

Tags

mCerulean

Expression

Bacterial

Mutation

D1109-D1275, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-CHES1L

Plasmid

#51836

Purpose

encodes the long isoform of human CHES1 and a Flag tag

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-MYC.Mm.KAT5

Plasmid

#184652

Purpose

Retroviral expression of mouse Kat5

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Kat5 (Kat5 Mouse)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-mApg5(K130R)

Plasmid

#22959

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

130 Lysine to Arginine

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
TFORF1471

Plasmid

#143814

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TOX2 (TOX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mGnptg

Plasmid

#78106

Purpose

Mammalian expression of mouse Gnptg with C-terminal His tag

Depositor

Thomas Braulke

Article

Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

Insert

Gnptg (Gnptg Mouse)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pET41a-MED19

Plasmid

#15430

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED19 (Med19 Mouse)

Tags

GST and His

Expression

Bacterial

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
374 pME-ets1a

Plasmid

#49000

Purpose

full length coding sequence for zebrafish ets1a gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

v-ets erythroblastosis virus E26 oncogene homolog 1a (ets1 Zebrafish)

Use

Gateway middle entry

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pSIL-eGFP-shACTN2

Plasmid

#52677

Purpose

shRNA against α-actinin-2 with eGFP transfection marker

Depositor

Johannes Hell

Article

Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

Insert

ACTN2 (Actn2 Rat)

Use

RNAi

Tags

CMV-EGFP

Promoter

U6

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only

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pSW683 - pAHP6>>

Plasmid

#115998

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pAHP6:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Tags

HDEL and SP(ER)

Expression

Plant

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pRC/CMV-JAK1P1084A-VSV

Plasmid

#139358

Purpose

Expression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)

Depositor

Sandra Pellegrini

Article

Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

Insert

JAK1 cDNA with 300nt of 5' UTR (JAK1 Human)

Tags

VSV

Expression

Mammalian

Mutation

P1084A

Promoter

CMV

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pETM33_KPNA4_Arm

Plasmid

#178495

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KPNA4_Arm (KPNA4 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pRSETC-RdgBbeta-splice variant 1

Plasmid

#58276

Purpose

bacterial expression of human RdgBbeta splice variant 1

Depositor

Shamshad Cockcroft

Article

Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

Insert

RdgBbeta (PITPNC1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET30a-SARS-CoV2-ORF3b (S23Q)

Plasmid

#165088

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)

Expression

Bacterial

Mutation

Stop codon to glutamine at 23rd amino acid

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-RNF8-N-terminus(1-111)

Plasmid

#64671

Purpose

Expresses only N terminus of RNF8, amino acids 1 to 111

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

ring finger protein 8 (RNF8 Human)

Tags

GFP

Expression

Mammalian

Mutation

containing only N-terminus amino acids 1 to 111

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pT7-gfap-sgRNA

Plasmid

#65566

Purpose

in vitro trancription of sgRNA targeting the zebrafish gfap locus

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

gfap sgRNA (gfap Zebrafish)

Use

In vitro rna transcription

Promoter

T7

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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DBP-bio

Plasmid

#68528

Purpose

Expresses full-length P. vivax DBP ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

Depositor

Julian Rayner, Gavin Wright

Article

Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

Insert

DBP

Tags

rat CD4 d3+4, enzymatic biotinylation sequence

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only