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pLV_PGK_3xHA-LTB4R(T308A,S310A)

Plasmid

#208094

Purpose

Mammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations with an N-terminal 3x HA tag.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

3xHA-LTB4R(T308A,S310A) (LTB4R Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T308A, S310A

Promoter

PGK

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-B4GALT3mut

Plasmid

#203602

Purpose

Reporter plasmid carrying B4GALT3 3'UTR with mutated miR27b target sequences.

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

beta-1,4-galactosyltransferase 3 (B4GALT3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Seed sequences in miR-27b target sequences were m…

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-MAIP1mut

Plasmid

#203604

Purpose

Reporter plasmid carrying MAIP1 3'UTR with mutated miR27b target sequences

Depositor

Hiroyuki Mizuguchi

Article

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

matrix AAA peptidase interacting protein 1 (MAIP1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

miR-27b target sequences in the 3'UTR was mu…

Promoter

SV40

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-Bat-SL-CoVZC45_PLP

Plasmid

#201939

Purpose

Expresses Bat-SL-CoVZC45 papain-like protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

Bat-SL-CoVZC45 PLP

Expression

Yeast

Promoter

GAL1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-HCV_NS3-GS-NS4A

Plasmid

#203504

Purpose

Expresses HCV NS3-NS4A protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCV NS3-GS-NS4A

Expression

Yeast

Promoter

GAL1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-Bat-SL-CoVZC45_3CL

Plasmid

#201944

Purpose

Expresses Bat-SL-CoVZC45 3CL protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

Bat-SL-CoVZC45 3CL protease

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-DUGV_OTU

Plasmid

#203463

Purpose

Expresses DUGV OTU domain of L protein from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

DUGV OTU domain of L protein (DUGVsLgp1 )

Expression

Yeast

Promoter

GAL10

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-3x-uC-HCoV-229E_3CL

Plasmid

#201937

Purpose

Expresses HCoV-229E 3CL protease from a GAL-3x promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCoV-229E 3CL protease

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-HPeV_3C

Plasmid

#203465

Purpose

Expresses HPeV 3C protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HPeV 3C protease (polyprotein gene )

Expression

Yeast

Promoter

GAL10

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF-G1TMSNKRS

Plasmid

#198323

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-CES2C-ΔC-S230A

Plasmid

#200557

Purpose

Mammalian expression of secreted mouse CES2C S230A mutant

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2C S230A mutant delta C-terminal (Ces2c Mouse)

Tags

6xHis, Flag, and V5

Expression

Mammalian

Promoter

CMV promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-F1.GS.CAR-3G

Plasmid

#194462

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-F1.218.CAR-3G

Plasmid

#194460

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_862-1384_R

Plasmid

#147392

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_862-1384

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_862-1384 (gw Nematode, Fly)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut-CIM2mut_R

Plasmid

#147396

Purpose

Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut (TNRC6C Nematode, Human)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2_254-400-DmGW182_862-1384_R

Plasmid

#147406

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_862-1384

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_862-1384 (gw Nematode, Fly)

Expression

Insect

Mutation

Deletion o f2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384_R

Plasmid

#147384

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384 (gw Nematode, Fly)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMeCP2Y pc644

Plasmid

#167565

Purpose

The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced YFP (pEYFP-N1 vector; CLONTECH Laboratories, Inc.)

Depositor

Cristina Cardoso

Article

Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

Insert

Methyl-CpG binding protein 2 (Mecp2 Rat)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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ppil2.280.457.Y389H

Plasmid

#137658

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

Insert

ppil2 (PPIL2 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

280-457-Y389H

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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ppil2.280.457.Y389W

Plasmid

#137657

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Davis et al PLoS Biol. 2010 Jul 27;8(7):e1000439. do

Insert

ppil2 (PPIL2 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

280-457-Y389W

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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