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pCDNA3-myc3-FBW5

Plasmid

#19905

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

F-box and WD repeat domain containing 5 (FBXW5 Human)

Tags

myc3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pMW357

Plasmid

#238204

Purpose

Expresses untagged TaTasR from a PBAD promoter, followed by the native TIGR array (8 units)

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Expression

Bacterial

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#16

Plasmid

#153524

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#16

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#16

Expression

Bacterial

Promoter

pBAD

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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dCBE4-gam

Plasmid

#124449

Purpose

Expresses dead Cas9 (dCas9)-CBE4-gam

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

dCBE4-gam

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

D10A, H840A

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pRR-GR-5Z

Plasmid

#23060

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

GR (NR3C1 Human)

Expression

Yeast

Available Since

March 16, 2010

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-NKX2-8

Plasmid

#75080

Purpose

retroviral vector holding human NKX2-8 cDNA

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

NKX2-8 (NKX2-8 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-LexAOx4-Pmin-Fluc-PGK-Rluc-P2A-mCherry

Plasmid

#192634

Purpose

Lentiviral vector for inducible Fluc expression with 4x LexA operator (LexAO) and constitutive Rluc and mCherry.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

firefly luciferase

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal promoter

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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T/brachyury(2A-Dre)

Plasmid

#140761

Purpose

Targeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Insert

Dre

Use

Mouse Targeting

Promoter

Endogenous T/brachyury

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

Plasmid

#89587

Purpose

For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nuclear localization signal (nls) turboRFP

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT S277G

Plasmid

#13615

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

Changed catalytic Serine 277 to Alanine

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pAV103

Plasmid

#63193

Purpose

pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Worm and Yeast

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCgVchcrRNA

Plasmid

#203815

Purpose

Expression of CRISPR RNA, shuttle vector for Corynebacterium glutamicum / E. coli

Depositor

Sheng Yang

Article

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

CRISPR(BsaI)

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-N-CretrcintG

Plasmid

#69572

Purpose

One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

Insert

kozak consensus - N-CretrcintG

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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Beta-Globin-PTC39

Plasmid

#130701

Purpose

Expresses Beta-globin-PTC39 NMD reporter (It has a Premature termination codon (PTC) at 39th Amino acid)

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

Beta Globin (HBB Human)

Expression

Mammalian

Mutation

PTC39

Promoter

Pcmv

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

Plasmid

#202571

Purpose

Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

LINE-1

Tags

GFP-AI retrotransposition reporter and HaloTag7 o…

Expression

Mammalian

Mutation

Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-C-CreintG

Plasmid

#69573

Purpose

One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

Insert

kozak consensus - C-CreintG

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-NAP1

Plasmid

#208862

Purpose

Stably express GFP-tagged NAP1 for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (AZI2 Human)

Tags

FKBP-GFP

Expression

Mammalian

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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M2e-noro-VLP

Plasmid

#201192

Purpose

Expresses noro-VLP decorated with influenza M2e in insect cells

Depositor

Vesa Hytönen

Article

Lampinen et al Front Cell Infect Microbiol. 2023 Jun 22

Insert

M2e-noro-VLP

Tags

Influenza M2e peptide

Expression

Insect

Promoter

Polyhedrin

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-Ms-c-Myc-W136E

Plasmid

#26024

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

c-Myc (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

W136E

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

Plasmid

#98974

Purpose

Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNAs pair 3 and Cas9(D10A)

Expression

Mammalian

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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