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TAPBPL pmRFP-N2 (721)

Plasmid

#62000

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

TAPBPL (TAPBPL Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ZNF736

Plasmid

#101602

Purpose

Donor Vector containing ZNF736 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF736 (ZNF736 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pRDA_122

Plasmid

#208095

Purpose

Perturb-Seq lentiviral gRNA expression vector with a 3' appended capture sequence to enable direct capture of CRISPR gRNAs for scRNA-seq

Depositor

John Doench

Article

Geiger-Schuller et al bioRxiv. 2023 Jan 24:2023.01.23.525198.

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EF1a

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLRC1-NEK10p:NEK10-3XFLAG

Plasmid

#137030

Purpose

Lentiviral vector for expressing NEK10 under the control of the endogenous NEK10 promoter

Depositor

David Sabatini

Article

Chivukula et al Nat Med. 2020 Jan 20. pii: 10.1038/s4159

Insert

NIMA-like kinase 10 (NEK10 Human)

Use

Lentiviral

Tags

3XFLAG

Promoter

human NEK10 endogenous promoter

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4 CMV GST mTagBFP

Plasmid

#206259

Purpose

ENTR Vector 4 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTagBFP localised to Golgi organelles under the control of CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

GTS mTagBFP

Use

Multimate/gateway entr 4

Tags

BFGT1 (Golgi localisation peptide)

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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GABPA_pLENTI-CAG-IRES-GFP

Plasmid

#176999

Purpose

Mammalian lentiviral expression vector encoding GABPA

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GABPA (GABPA Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF574

Plasmid

#88644

Purpose

Donor Vector containing ZNF574 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF574 (ZNF574 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-CAT-L713F

Plasmid

#173944

Purpose

Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CAT-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF706

Plasmid

#88037

Purpose

Donor Vector containing ZNF706 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF706 (ZNF706 Human)

Use

Gateway donor vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF251

Plasmid

#101451

Purpose

Donor Vector containing ZNF251 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF251 (ZNF251 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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