We narrowed to 61,146 results for: %s
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Plasmid#202571PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
TagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Methionine 91 to Alanine, ORF1p Glu…Available SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-C-CreintG
Plasmid#69573PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cellsDepositorInsertkozak consensus - C-CreintG
UseSynthetic BiologyExpressionMammalianPromoterCAGAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-NAP1
Plasmid#208862PurposeStably express GFP-tagged NAP1 for CID induced mitophagyDepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
M2e-noro-VLP
Plasmid#201192PurposeExpresses noro-VLP decorated with influenza M2e in insect cellsDepositorInsertM2e-noro-VLP
TagsInfluenza M2e peptideExpressionInsectPromoterPolyhedrinAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Ms-c-Myc-W136E
Plasmid#26024DepositorAvailable SinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3
Plasmid#98974PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 3 and Cas9(D10A)
ExpressionMammalianAvailable SinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
CSIV-TRE-ASCL1
Plasmid#154883PurposeDoxycycline-dependently expresses human ASCL1.DepositorInsertASCL1 (ASCL1 Human)
UseLentiviralAvailable SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP71-mGFP-LLO-mPMEL
Plasmid#174608Purposeexpresses palmitoylated GFP with a C terminal fusion of the LLO190 epitope and the murine hPMEL epitopeDepositorInsertmembrane GFP fused to LLO and mouse PMEL antigen
ExpressionMammalianAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
gRNA_tdtomato_reporter1_SP-2x
Plasmid#79370PurposeAssays activity of transcriptional activators fused to SP Cas9DepositorInserttdtomato
UseCRISPRExpressionMammalianAvailable SinceApril 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pP2A_NLS_sfGFP
Plasmid#63709PurposeExpressing P2A_NLS_sfGFP with homologous arm of Nanog.DepositorInsertNanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm
UseJust a donor dna with nanog homologous arm for kn…TagssfGFPAvailable SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔKinase
Plasmid#163404PurposeExpresses BMPR-2 lacking the kinase domain in mammalian cellsDepositorAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB3K3 containing mRFP1
Plasmid#118083PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertpromoter, RBS, mRFP1
UseEscherichia coliAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3 containing mRFP1
Plasmid#118082PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertpromoter, RBS, mRFP1
UseEscherichia coliAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18
Plasmid#172316PurposeCRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGA
Plasmid#153530PurposeUsed as a donor vector for the targeted correction of a mutation in PIGA exon 6DepositorInsertPIGA (a 1,991-bp fragment from intron 5 and exon 6)
UseDonor plasmidTagsN/AMutationNo mutationPromoterNo promoterAvailable SinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#42
Plasmid#153525PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#42DepositorInsertSb#42
ExpressionBacterialPromoterpBADAvailable SinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
eNme2-C TadDE
Plasmid#193842PurposeExpress TadDE (with eNme2-C variant) in mammalian cellsDepositorInserteNme2-C TadDE
ExpressionMammalianAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
GW1-mito-roGFP1-mApple
Plasmid#107218PurposeMammalian expression plasmid to target the roGFP1-mApple sensor to the mitochondrial matrix.DepositorInsertroGFP1-mApple
Tags4xCox8ExpressionMammalianPromoterCMVAvailable SinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
SspB-HaloTag-DHPH-TIAM1
Plasmid#176114PurposeHeterodimerization with iLID, Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
ExpressionMammalianAvailable SinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHLP701
Plasmid#203743PurposeC-terminal tagging of target proteins with 3xV5-IAA17 degronDepositorInsertIAA17 auxin binding domain
UsePcr amplification template for gene taggingTags3xV5PromoternoneAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only