We narrowed to 15,772 results for: LEA

1. pNLS-iRFP670

   Plasmid

   #45466

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP670
   

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

   Promoter

   CMV

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNLS-iRFP713

   Plasmid

   #45468

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP713
   

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   T2A, S13L, A92T, V104T, V114I, E161K, E193K, F198…

   Promoter

   CMV

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMVMagFRET-1

   Plasmid

   #50742

   Purpose

   Expresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.

   Depositor

   Maarten Merkx

   Article

   Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

   Insert

   gene encoding for MagFRET-1
   

   Expression

   Mammalian

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. JDW 1042 (pLenti6.2_CMV_V5_TCF7L1_P2A_H2A_mCherry)

   Plasmid

   #233540

   Purpose

   A CMV driven human TCF7L1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   TCF7L1 (TCF7L1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMEXC3H

   Plasmid

   #110080

   Purpose

   Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3C cleavage site - His10 Tag

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. sox10:mCherry

   Plasmid

   #98695

   Purpose

   I-SceI meganuclease-mediated mCherry transgenesis in zebrafish

   Depositor

   Thomas Look

   Article

   Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

   Insert

   mCherry
   

   Use

   Zebrafish expression

   Available Since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBA2219

   Plasmid

   #38152

   Depositor

   David Waugh

   Article

   Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

   Insert

   DsbC (dsbC E. coli)
   

   Tags

   N-terminal leader peptide (cloning artifact)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJExpress414_GmPOPB

   Plasmid

   #92234

   Purpose

   Express wild type prolyl oligopeptidase from Galerina marginata

   Depositor

   James Naismith

   Article

   Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

   Insert

   Prolyl oligopeptidase B from Galerina marginata
   

   Tags

   6x-Histag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7 promoter

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4-ZfCry4

   Plasmid

   #136242

   Purpose

   Protein expression of Zebrafish (Zf) cytochrome 4 with a C terminal FLAG tag

   Depositor

   Aziz Sancar

   Article

   Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

   Insert

   ZfCry4
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D196H- Please see depositor comments below

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. GCEPIA1-SNAPER

    Plasmid

    #172659

    Purpose

    Detect the real-time ER calcium store and local calcium microdomains in live cells.

    Depositor

    Pingyong Xu

    Article

    Luo et al Biophys Rep (2019) 5, 31–42

    Insert

    calmodulin
    

    Expression

    Mammalian

    Mutation

    Please see depositor comments

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO_HaloTag7_LamB1

    Plasmid

    #175528

    Purpose

    Expression of HaloTag7 at the nuclear lamina of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag7-LamB1
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNLS-iRFP720

    Plasmid

    #45467

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP720
    

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. LeGO-lnc

    Plasmid

    #80624

    Purpose

    Lentiviral lncRNA Overexpression

    Depositor

    Jan-Henning Klusmann

    Article

    Emmrich et al Mol Cancer. 2014 Jul 15;13:171. doi: 10.

    Insert

    empty control
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pREXC3GH

    Plasmid

    #47077

    Purpose

    FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Lactococcus lactis expression

    Tags

    3C protease site, GFP, and His

    Promoter

    NIS (nisin)

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKM493

    Plasmid

    #140191

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    TEV-Flag-Gly4-eGFP
    

    Tags

    Flag + eGFP

    Expression

    Bacterial

    Promoter

    PGroEL

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEMHE-NLS-mEGFP

    Plasmid

    #105527

    Purpose

    T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Nucleoplasmin
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Mutation

    NLS of nucleoplasmin (encoding amino acids KRPAAT…

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWT35S-Citrine-NLS

    Plasmid

    #182583

    Purpose

    Transient expression of Citrine-NLS in plant cell (Nucleus)

    Depositor

    Yutaka Kodama

    Article

    Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

    Insert

    NLS
    

    Tags

    Citrine

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. MCS+

    Plasmid

    #90412

    Purpose

    A FRET sensor that report the electric potential at the inner leaflet of cell plasma membrane

    Depositor

    Katharina Gaus

    Article

    Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103

    Insert

    Lck10+ Venus+mcherry+ strech of positively charged amino acids
    

    Expression

    Mammalian

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. iMbody_pSANG10-3F

    Plasmid

    #184496

    Purpose

    Expresses iMbody-6xHis-3xFLAG in BL21(DE3) bacteria

    Depositor

    Marcel Dinger

    Article

    Zeraati et al STAR Protoc. 2023 Nov 21;4(4):102729. do

    Insert

    iMbody
    

    Tags

    3xFLAG, 6xHis, and pelB leader

    Expression

    Bacterial

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEmerald-LaminA-C-18

    Plasmid

    #54138

    Purpose

    Localization: Nuclear Envelope, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    LaminA (LMNA Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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