We narrowed to 13,836 results for: ckb

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pEG BacMam C term 3C eGFP myc

Plasmid

#160685

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

His8 eGFP 3C

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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3'-3xFLAG minimal CMV pCDNA5

Plasmid

#212045

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pBT344_pattB-pCA-GFP-pA-FRT5

Plasmid

#52550

Purpose

for Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing mice

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly-RpS12

Plasmid

#71504

Purpose

flySTARR-seq screening vector with RpS12 core promoter

Depositor

Alexander Stark

Article

Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pYFAC

Plasmid

#168982

Purpose

Filamentous fungi vector. Also referred to as pYFAC-pyrG.

Depositor

Yit Heng Chooi

Article

Chooi et al Environ Microbiol. 2017 May;19(5):1975-1

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in fil…

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDM1513

Plasmid

#108998

Purpose

safe haven knock-in plasmid for the act5 locus, N-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus, n…

Tags

GFP

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCAT.015

Plasmid

#119558

Purpose

Level T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMpGWB100

Plasmid

#68062

Purpose

MCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSB700 HL1gR4

Plasmid

#124450

Purpose

Expresses gRNA HL1 gR4 in the pSB700 backbone

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

HL1 gR4

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pL157A-EGFP

Plasmid

#68850

Purpose

mutated OPTN was inserted into pEGFP-N1 backbone

Depositor

Beatrice Yue

Article

Turturro et al Springerplus. 2014 Feb 19;3:99. doi: 10.

Insert

optineurin (OPTN Human)

Tags

EGFP

Expression

Mammalian

Mutation

L157A, mutation has not been linked to any diseas…

Promoter

CMV

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only
U6::sgBFP-U6::sgKrasG12D-pX333-red

Plasmid

#149448

Purpose

pX333 backbone expressing 2 sgRNA targeting BFP and KRAS G12D to edit mouse KRAS G12D allele.

Depositor

Jonathan Chernoff

Article

Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

Insert

sgRNA BFP and mouse KRAS G12D

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWY

Plasmid

#112901

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mCitrine.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pgRNA-guideless

Plasmid

#129464

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Guideless gRNA backbone

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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SlugBE4max

Plasmid

#163799

Purpose

Expresses AncBE4max and nSlugCas9

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Mutation

SlugCas9 (D10A)

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-hPDIb′a′

Plasmid

#60556

Purpose

enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

Depositor

Han Choe

Article

Song et al Appl Biochem Biotechnol. 2013 May;170(1)

Type

Empty backbone

Tags

His6-hPDIb'a' tag

Expression

Bacterial

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pDM1515

Plasmid

#109000

Purpose

safe haven knock-in plasmid for the act5 locus, C-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus, c…

Tags

GFP

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGWB328

Plasmid

#68656

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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YPRCΔ15α1

Plasmid

#101712

Purpose

GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Type

Empty backbone

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRPa-iSL(MCS1+2)

Plasmid

#69244

Purpose

Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

Depositor

David Horn

Article

Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

Type

Empty backbone

Use

Expression in trypanosoma brucei

Promoter

RRNA promoter

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - DDX3Y sgRNA 2

Plasmid

#70657

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against DDX3Y

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only