We narrowed to 15,772 results for: LEA
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Plasmid#59933PurposegRNA for cleavage at dpy-10(cn64) locus in C elegansDepositorInsertdpy-10(cn64) gRNA
UseCRISPRPromoterU6Available SinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMEXNH3
Plasmid#110079PurposeAnalogous to pMINTNH3, but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneTagsHis10 Tag - 3C cleavage siteExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE60 MGH
Plasmid#204244PurposeBacterial expression of a fusion of watermelon glyoxysomal malate dehydrogenase (MDH) and GFP with His tag; Use in CURES (integration of research into undergraduate teaching labs).InsertMGH
Tags6X His and GFPExpressionBacterialMutationfusion of MDH and GFP with His tagPromoterLacAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-deltaOVA
Plasmid#64595PurposeExpression of N-terminally truncated chicken ovalbumin as model antigen for study of MHC class I and class II presentationDepositorInsertOvalbumin
ExpressionMammalianMutationLacking first 49 aa (leader sequence for transloc…PromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4)
Plasmid#211861PurposeNuclear-localized E3-DART ligase in domestication vector.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HAAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQdCas12a.luxR(mut)-sggfp(C)
Plasmid#236043PurposeThe plasmid pQdCas12a.luxR(mut)-sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.DepositorInsertquorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
UseCRISPRPromoterquorum sensing promoterAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
XXBLM (XXBLMA-c075)
Plasmid#98223PurposeVector for periplasmic expression of Lama antibodies with PelA leader and C-terminal His6 tagDepositorAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJA42
Plasmid#59930PurposegRNA for cleavage at rol-6(su1006) locus in C elegansDepositorInsertrol-6(su1006) gRNA
UseCRISPRPromoterU6Available SinceSept. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3CA
Plasmid#47069PurposeFX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tagDepositorTypeEmpty backboneTags3C protease site (PreScission site), AVI-tag, and…ExpressionBacterialPromoteraraBADAvailable SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEL2108
Plasmid#201969PurposeORBIT integrating plasmid for C-terminal tagging with eGFP containing a 3C protease cleavage site.DepositorInsertattB-3C-eGFP-4xGly-TEV-Flag-6xHis
UseOrbit integrating plasmidAvailable SinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCHAP2
Plasmid#206847PurposeA plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP1
Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNSEN-d2
Plasmid#59763PurposeExpresses nuclear EGFP and Tomato in mammalian cellsDepositorInsertsd2-EGFP
Tomato
Tags3X NLS and PESTExpressionMammalianPromoterCMV promoter and SV40 promoterAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4134100
Plasmid#152050PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHis-003ExpressionMammalianMutationwtPromoterCMVAvailable SinceMay 20, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
csr-1_hH1_mEos3.1_V5
Plasmid#191750PurposePhotoconvertible fluorescent nuclear reporter.DepositorAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDB4280
Plasmid#98699PurposeA Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 systemDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLICER
Plasmid#197288PurposeConjugative plasmid that facilitates seamless deletions in D. radiodurans following insertion of a seamless deletion cassette. Contains an origin of transferDepositorInsertI-SceI Endonuclease
UseSynthetic Biology; Bacterial and yeast cloningAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-LgBiT-CaM
Plasmid#183401PurposeExpresses nuclear-localized LgBiT-CaMDepositorInsertLgBiT-CaM
TagsSV40 NLSExpressionMammalianPromoterCMVAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
438-GST
Plasmid#97092PurposeGST-tev-yORFDepositorTypeEmpty backboneTagsGST tag and TEV cleavage siteExpressionInsectPromoterPolyhedrin promoterAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
CBX7
Plasmid#25241PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits