We narrowed to 12,029 results for: LEA
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Plasmid#176255PurposepNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2DepositorInserthumanized fnCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseMutationD917A and E1006A mutations to inactivate the endo…Available SinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-30aa-Noxa-4E-pEGFP-C1
Plasmid#167529PurposeTo increase the length of the linker between Venus and Noxa-4E proteinsDepositorInsertPMA-induced protein 1 (PMAIP1 Human)
TagsVenusExpressionMammalianMutationC25E, L29E, F32E, L36EPromoterCMVAvailable SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-wzzBST
Plasmid#172968PurposepWSK29 with Salmonella typhimurium wzzBST geneDepositorInsertwzzB gene from Salmonella enterica serovar Typhimurium
ExpressionBacterialMutationK198E- please see depositor commentsAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWSK29-fepEST
Plasmid#172969PurposepWSK29 with Salmonella typhimurium fepEST geneDepositorInsertfepE gene from Salmonella enterica serovar Typhimurium
ExpressionBacterialMutationG160S- please see depositor commentsAvailable SinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY1(L106P)
Plasmid#166714Purposeexpresses AMOT(L106P) in mammalian cellsDepositorInsertAngiomotin p130 L106P (AMOT Human)
Tags2xHAExpressionMammalianMutationchanged leucine 106 to prolinePromoterCMV promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-AMOT p130∆PPxY1(A112K)
Plasmid#166715Purposeexpresses AMOT(A112K) in mammalian cellsDepositorInsertAngiomotin p130 A112K (AMOT Human)
Tags2xHAExpressionMammalianMutationchanged alanine 112 to lysinePromoterCMV promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW1(193-226)
Plasmid#166701Purposeexpresses NEDD4L WW1 in bacterial cellsDepositorInsertNEDD4L WW1 domain
TagsGST, HRV 3C site, and Thrombin siteExpressionBacterialPromotertac promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW2(385-418)
Plasmid#166702Purposeexpresses NEDD4L WW2 in bacterial cellsDepositorInsertNEDD4L WW2 domain
TagsGST, HRV 3C site, and Thrombin siteExpressionBacterialPromotertac promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW3(497-530)
Plasmid#166703Purposeexpresses NEDD4L WW3 in bacterial cellsDepositorInsertNEDD4L WW3 domain
TagsGST, HRV 3C site, and Thrombin siteExpressionBacterialPromotertac promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PP-NEDD4L-WW4(548-581)
Plasmid#166704Purposeexpresses NEDD4L WW4 in bacterial cellsDepositorInsertNEDD4L WW4 domain
TagsGST, HRV 3C site, and Thrombin siteExpressionBacterialPromotertac promoterAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-10xPP7-Qb model based
Plasmid#158203PurposeCassette of 10 different binding sites with high affinity to both PP7 and Qb phage coats protein, as computed by the ML model.DepositorInsert10 binding sites with high affinity to PP7 and QB coat proteins based on the machine-learning model
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
mWasabi-ADAP1
Plasmid#163906PurposeFull length mouse ADAP1 with N terminal fluorescent tag for purification from insect cellsDepositorAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)
Plasmid#149350PurposeMammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNADepositorInsertSpyCas9
UseCRISPRTagsFlag tagExpressionMammalianPromoterCbhAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ad-HisFlag-USP19ER
Plasmid#155244Purposefor generating adenoviruses expressing the ER isoform of rat USP19DepositorInsertUSP19 (ER isoform) (Usp19 Rat)
UseAdenoviralTags6XHis-3XFlagMutationH118R- please see dep. commentsPromoterCMVAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shCenpf
Plasmid#160956PurposeCenpf shRNA in pMKO.1 retroviral vectorDepositorAvailable SinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4134515
Plasmid#152318PurposeMammalian expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;CBPExpressionMammalianMutationwtPromoterCMVAvailable SinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4134513
Plasmid#152314PurposeMammalian expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHis-003ExpressionMammalianMutationwtPromoterCMVAvailable SinceSept. 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4134473
Plasmid#152302PurposeMammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;V5ExpressionMammalianMutationwtPromoterCMVAvailable SinceSept. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET15-His-∆NGlpG(Y205F)
Plasmid#155132PurposeExpresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
TagsHisExpressionBacterialMutationY205F, insert codes for GlpG residues 87-276PromoterT7Available SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM33
Plasmid#154167PurposeAedes aegypti Rel2 (long isoform) with C-terminal IκB domain deleted, expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorInsertRel2 (long isoform)
TagsFLAGExpressionInsectMutationC-terminal IκB domain deletedPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only