We narrowed to 18,439 results for: STO

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SpyTag-Kraken Quartet

Plasmid

#214730

Purpose

In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 XBB.1.5 separated by GS-linkers with an N-terminal SpyTag

Depositor

Mark Howarth

Article

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

SpyTag-Kraken Quartet (S Synthetic)

Tags

SpyTag

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag-Quartet_NoLinker

Plasmid

#214731

Purpose

In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 with no linkers between the RBDs and an N-terminal SpyTag

Depositor

Mark Howarth

Article

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

SpyTag-Quartet_NoLinker (S Synthetic)

Tags

SpyTag

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag-RBD Delta

Plasmid

#214723

Purpose

Expresses SARS-CoV-2 Delta RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Article

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

Receptor-Binding Domain (S SARS-CoV-2)

Tags

SpyTag

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD4-AroPLUS_LITE_PATCHED

Plasmid

#215585

Purpose

For overexpression of Gal4-DBD HOXD4 AroPLUS LITE PATCHED

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroPLUS LITE PATCHED (HOXD4 Human)

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR223-CMV-Cre-U6-sgC0111-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

Plasmid

#209062

Purpose

Entry vector that encodes sgRNAs against mouse Rb1, Trp53, Rbl2, and a non-targeting sgRNA (C0111) and CMV Cre recombinase.

Depositor

Matthew Oser

Article

Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

Insert

non-targeting sgRNA (C0111)

Use

Gateway vector to be used for lr reaction

Promoter

U6

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet_Seq1.3

Plasmid

#206137

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1 (Col1a1 Mouse)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-FES

Plasmid

#203501

Purpose

Expresses human FES kinase from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

FES (FES Human)

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET-hMICU1EF

Plasmid

#199180

Purpose

This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.

Depositor

Ming-Feng Tsai

Article

Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

MICU1 (MICU1 Human)

Tags

His6 and MBP, Thrombin site, TEV site

Mutation

D421A, E432K, F433W, F453W

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO mouse METAP1 1-79

Plasmid

#197397

Purpose

Expresses SUMO-tagged mouse METAP1, residues 1-79

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

METAP1 1-79 (Metap1 Mouse)

Tags

SUMO

Expression

Bacterial

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO mouse METAP1 1-59

Plasmid

#197396

Purpose

Expresses SUMO-tagged mouse METAP1, residues 1-59

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

METAP1 1-59 (Metap1 Mouse)

Tags

SUMO

Expression

Bacterial

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-Inducible Mouse C325A Caspase-9 Mutant

Plasmid

#194500

Purpose

Inducible Expression of Mouse C325A Caspase-9 Mutant

Depositor

Hannah Rabinowich

Article

Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

Insert

Mouse Caspase-9 C325A Mutant (Casp9 Mouse)

Tags

None

Expression

Mammalian

Mutation

Changed Cysteine 325 to Alanine to eliminate prot…

Promoter

CMV

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-inducible 3xFlag Mouse C325A Caspase-9 Mutant

Plasmid

#194501

Purpose

Inducible Expression of N-Terminal 3X FLAG Mouse Caspase-9 C325A Mutant

Depositor

Hannah Rabinowich

Article

Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

Insert

Caspase-9 C325A Mutant (Casp9 Mouse)

Tags

3X Flag

Expression

Mammalian

Mutation

Changed Cysteine 325 to Alanine to eliminate prot…

Promoter

CMV

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k sgRNA-2 PX459 plasmid

Plasmid

#192256

Purpose

expressing sgRNA-2 targeting eIF3k locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3k locus closed to stop coden (EIF3K Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k sgRNA-1 PX459 plasmid

Plasmid

#192255

Purpose

expressing sgRNA-1 targeting eIF3k locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3k locus closed to stop coden (EIF3K Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f sgRNA-1 PX459 plasmid

Plasmid

#192253

Purpose

expressing sgRNA-1 targeting eIF3f locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3f locus closed to stop coden (EIF3F Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f sgRNA-2 PX459 plasmid

Plasmid

#192254

Purpose

expressing sgRNA-2 targeting eIF3f locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3f locus closed to stop coden (EIF3F Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b sgRNA-2 PX459 plasmid

Plasmid

#192250

Purpose

expressing sgRNA-2 targeting eIF3b locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3b locus closed to stop coden (EIF3B Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b sgRNA-1 PX459 plasmid

Plasmid

#192249

Purpose

expressing sgRNA-1 targeting eIF3b locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3b locus closed to stop coden (EIF3B Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Neo

Plasmid

#186672

Purpose

Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Neomycin (YTHDF2 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_ANKRD1_KO_Hygro

Plasmid

#186668

Purpose

Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO Hygromycin (ANKRD1 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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