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Plasmid#85072PurposeGreen/red FRET biosensor for RhoA in the GTP locked constitutively active stateDepositorInsertRhoA Biosensor constitutively active (Q63L) (RHOA Human)
UseTags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…MutationQ63LPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET mNG-GECO1
Plasmid#201933PurposeExpresses mNG-GECO1 indicator in bacteriaDepositorInsertmNeonGreenGECO1
UseTagsHIS tagExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
E0040m (GFP)_CD
Plasmid#66032PurposeMoClo Basic Part: CDS - Fluorescent protein. Green. Modified from Bba_E0040 to fix illegal site. [C:E0040m:D]DepositorInsertFluorescent reporter - GFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWKS11
Plasmid#139418PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mNeonGreen marker.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-His6-GFP-C-LACE
Plasmid#133913PurposeBacterial expression of His6-GFP-C-LACEDepositorInsertsuperfolder green fluorescent protein
UseTagsLACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…ExpressionBacterialMutationPromoterT7Available sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pENTR4-GFP-C3 (w393-1)
Plasmid#17397PurposeGateway entry vector for N-term eGFP fusion, frame C3DepositorInsertGFP-C3
UseEntry vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCS2_hMGFP-60A
Plasmid#200393PurposeA plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.DepositorInsertmonster GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-wt-EGFP
Plasmid#191117PurposeAn wt EGFP control for ZebrafishDepositorInsertenhanced green fluorescence protein
UseTagsHis tagExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-sfGFP-DocI
Plasmid#58708PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertsfGFP-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR/pTER shEGFP #1 (628-2)
Plasmid#17470PurposeGateway entry vector for EGFPshRNA#1 under H1/TO promoterDepositorInsertEnhanced green fluorescent protein shRNA #1
UseRNAi; Entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pENTR/pTER shEGFP #2 (728-2)
Plasmid#17471PurposeGateway entry vector for EGFPshRNA#2 under H1/TO promoterDepositorInsertEnhanced green fluorescent protein shRNA #2
UseRNAi; Entry vectorTagsExpressionMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLei-wt-sfGFP
Plasmid#191113PurposeAn E. coli sfGFP wild type controlDepositorInsertsuperfolder green fluorescence protein
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHL1530
Plasmid#37569DepositorInsertsGFP
TetR
CsrA
UseSynthetic BiologyTagsglgC leader regionExpressionBacterialMutationstart codon removedPromoterpConNoHind and pLlacO-1Available sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHL1355
Plasmid#37566DepositorInsertsGFP
TetR
CsrA
UseSynthetic BiologyTagsglgC leader regionExpressionBacterialMutationstart codon removedPromoterpConNoHind and pConNoHindM12Available sinceJuly 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET19b-EcMscL-TEV-mEGFP
Plasmid#165097PurposeExpresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.DepositorInsertE. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
UseSynthetic BiologyTagsmonomeric Enhanced Green Fluorescent ProteinExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
anxA9-GFP
Plasmid#107198PurposeExpresses human annexin A9-GFP fusion protein for live cell imagingDepositorInsertannexin A9 (ANXA9 Human)
UseTagsGreen Fluorescent ProteinExpressionMammalianMutationPromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPEE47
Plasmid#139426PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
anxA1-GFP
Plasmid#107194PurposeExpresses porcine annexin A1-GFP fusion protein for live cell imagingDepositorInsertannexin A1
UseTagsGreen Fluorescent ProteinExpressionMammalianMutationPromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-AlbEx14-mNG
Plasmid#201780Purposeknocks in mNeonGreen into exon 14 of Alb to produce Alb-mNeonGreen fusion protein in miceDepositorUseAAV and CRISPRTagsExpressionMutationPromoterU6Available sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGD283
Plasmid#129733PurposeDestination plasmid containing the 35S:XVE estradiol inducible driver and the A-G overhangs for GreenGate cloning of an expression casette for estradiole-inducible expression in plants.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only