We narrowed to 1,138 results for: fret

1. pcDNA3-InPGrp-Kras

   Plasmid

   #236001

   Purpose

   Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to plasma membrane.

   Depositor

   Jin Zhang

   Article

   Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

   Insert

   InPGrp-Kras (Cyth3 Synthetic, Rat)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Desmoplakin I tension sensor (F40-based)

   Plasmid

   #119186

   Purpose

   The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between mTFP1 and mEYFP.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakin I-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   inserted F40-based tension sensor module after aa…

   Promoter

   TCE

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-nAChr-Alpha6-GFP

   Plasmid

   #50486

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Tags

   GFP fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Mutation

   Gly-Ala-Gly flexible linker flanking the GFP open…

   Promoter

   CMV

   Available Since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1(+)-NES-ZapCV2 (cpV143)

   Plasmid

   #112060

   Purpose

   Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

   Depositor

   Amy Palmer

   Article

   Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

   Insert

   NES-ZapCV2
   

   Tags

   Nuclear Export Signal (NES) derived from HIV-1

   Expression

   Mammalian

   Mutation

   ECFP gene is truncated 36 bp at 3' end. Venu…

   Promoter

   CMV

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Epac-S-H21

   Plasmid

   #170335

   Purpose

   CFP(ST)_EPAC full length_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.

   Insert

   CFP(ST)_EPAC full length_Y(UST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Epac-S-H30

   Plasmid

   #170337

   Purpose

   CFP(UST)_EPACdDEPCD_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.

   Insert

   CFP(UST)_EPACdDEPCD_Y(UST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Epac-S-H27

   Plasmid

   #170336

   Purpose

   CFP(ST)_EPACdDEP _Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.

   Insert

   CFP(ST)_EPACdDEP _Y(UST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain

   Promoter

   CMV

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps

   Plasmid

   #181956

   Purpose

   Genetically encoded FRET-based cAMP indicator fused to AKAP79.

   Depositor

   Jin Zhang

   Article

   Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

   Insert

   AKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)
   

   Tags

   AKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Desmoplakin I no force control (F40-based)

   Plasmid

   #119187

   Purpose

   The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between mTFP1 and mEYFP that are tension-independent.

   Depositor

   Alex Dunn

   Article

   Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

   Insert

   human Desmoplakinhuman Desmoplakin I (1-1945)-[mTFP1-F40-mEYFP] (DSP Human)
   

   Use

   Transposon

   Tags

   mTFP1-F40-mEYFP

   Expression

   Mammalian

   Mutation

   truncation after aa1945

   Promoter

   TCE

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-2dV-Camui (T286A)

    Plasmid

    #220367

    Purpose

    T286A phosphodead mutant of pCAG-2dV-Camui

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T286A)-mEGFP(A206K) (Camk2a Synthetic, Rat)
    

    Tags

    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Alanine

    Promoter

    CAG

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Desmoplakin II tension sensor (F40-based)

    Plasmid

    #118715

    Purpose

    The F40-based human desmoplakin II tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II-[YPet(short)-F40-mCherry] (internal-1353) (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-F40-mCherry

    Expression

    Mammalian

    Mutation

    inserted F40-based tension sensor module after aa…

    Promoter

    CMV

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSBbi-Pur-mCerulean3-cpVenus[E172]

    Plasmid

    #125203

    Purpose

    SB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET high control

    Depositor

    Eric Davis

    Article

    Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

    Insert

    mCerulean3-cpVenus[E172]
    

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Desmoplakin II tension sensor (FL-based)

    Plasmid

    #118721

    Purpose

    The FL-based human desmoplakin II tension sensor detects forces in the range of 3-5 pN by changes in FRET between YPet(short) and mCherry.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II-[YPet(short)-FL-mCherry] (internal-1353) (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-FL-mCherry

    Expression

    Mammalian

    Mutation

    inserted FL-based tension sensor module after aa1…

    Promoter

    CMV

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Desmoplakin I tension sensor (F40-based)

    Plasmid

    #118725

    Purpose

    The F40-based human desmoplakin I tension sensor detects forces in the range of 1-6 pN by changes in FRET between YPet(short) and mCherry.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin I-[YPet(short)-F40-mCherry] (internal-1952) (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-F40-mCherry

    Expression

    Mammalian

    Mutation

    inserted F40-based tension sensor module after aa…

    Promoter

    CMV

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSBbi-Pur-cpVenus[E172]

    Plasmid

    #125202

    Purpose

    SB-transposon plasmid for stable expression of cpVenus[E172] cDNA as a FRET single color control

    Depositor

    Eric Davis

    Article

    Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

    Insert

    cpVenus[E172]
    

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSBbi-Pur-mCerulean3-spacer-cpVenus[E172]

    Plasmid

    #125204

    Purpose

    SB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET low control

    Depositor

    Eric Davis

    Article

    Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

    Insert

    mCerulean3-spacer-cpVenus[E172]
    

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Desmoplakin II no force control (F40-based)

    Plasmid

    #118716

    Purpose

    The no force control for the F40-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry] (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-F40-mCherry

    Expression

    Mammalian

    Mutation

    truncation after aa1353

    Promoter

    CMV

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-nAChr-Alpha6-YFP

    Plasmid

    #50483

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Tags

    YFP fusion in M3-M4 loop (after residue A405)

    Expression

    Mammalian

    Mutation

    Gly-Ala-Gly flexible linker flanking the YFP open…

    Promoter

    CMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

19. Desmoplakin II no force control (FL-based)

    Plasmid

    #118724

    Purpose

    The no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.

    Depositor

    Carsten Grashoff

    Article

    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

    Insert

    human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)
    

    Use

    Retroviral

    Tags

    YPet(short)-FL-mCherry

    Expression

    Mammalian

    Mutation

    truncation after aa1353

    Promoter

    CMV

    Available Since

    Dec. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Endo-InPGrp

    Plasmid

    #236003

    Purpose

    Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to endosomes.

    Depositor

    Jin Zhang

    Article

    Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

    Insert

    Endo-InPGrp (Cyth3 Synthetic, Rat)
    

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger), 6xHIS -…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge