We narrowed to 5,906 results for: pcDNA 3.1

1. pY112 (pcDNA3.1-huSaCpf1)

   Plasmid

   #92269

   Purpose

   Expression of humanized SaCpf1

   Depositor

   Feng Zhang

   Insert

   huSaCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY114 (pcDNA3.1-huPgCpf1)

   Plasmid

   #92273

   Purpose

   Expression of humanized PgCpf1

   Depositor

   Feng Zhang

   Insert

   huPgCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pY122 (pcDNA3.1-huCPbCpf1)

   Plasmid

   #92298

   Purpose

   Expression of humanized CPbCpf1

   Depositor

   Feng Zhang

   Insert

   huCPbCpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pY118 (pcDNA3.1-huLb4Cpf1)

   Plasmid

   #92294

   Purpose

   Expression of humanized Lb4Cpf1

   Depositor

   Feng Zhang

   Insert

   huLb4Cpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Gα13(EE)-YFP

   Plasmid

   #171374

   Purpose

   Plasmid encoding Gα13 internally-tagged with the Citrine variant of YFP in the b/c loop

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Ga13(EE)-YFP
   

   Use

   Tags

   Glu-Glu

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 (-) hYAP 1-1alpha

   Plasmid

   #124139

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Marius Sudol, Megan Finch-Edmondson

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   None (Wild Type)

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-ERBB4 (R544W)

   Plasmid

   #29531

   Depositor

   Yardena Samuels

   Article

   Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

   Insert

   ERBB4 (ERBB4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed arginine 544 to tryptophan

   Promoter

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1/3xmyc_NLRP11-NACHT

   Plasmid

   #131199

   Purpose

   mammalian expression

   Depositor

   Thomas Kufer

   Article

   Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

   Insert

   NACHT of NLRP11 (NLRP11 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   contains only the NACHT domain (amino acids 125-5…

   Promoter

   pCMV

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.1 AT1AR-324delta

   Plasmid

   #45635

   Depositor

   Robert Lefkowitz

   Article

   Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

   Insert

   AT1AR-324delta (Agtr1a Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   added stop codon at 324 aa

   Promoter

   CMV

   Available since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1(+)MBII-85-5'/3'ss mut

    Plasmid

    #67645

    Purpose

    MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 5' and 3' splice sites were mutated to consensus sequences.

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

    Insert

    MBII85 snoRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5' and 3' splice sites mutations

    Promoter

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Myc-gamma3 (in pCDNA3.1)

    Plasmid

    #118041

    Purpose

    GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG3 (Gabrg3 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Myc-gamma1 (in pCDNA3.1)

    Plasmid

    #118038

    Purpose

    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG1 (Gabrg1 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-Nluc(W12stop)

    Plasmid

    #180502

    Purpose

    Mammalian expression of Nluc(W12stop)

    Depositor

    Masaharu Somiya

    Article

    Somiya et al bioRxiv 2022.01.25.477620

    Insert

    Nluc(W12stop)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    W12(UGG) to amber stop codon(UAG)

    Promoter

    CMV

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-FluoSTEP-EKAR

    Plasmid

    #181858

    Purpose

    Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

    Insert

    FluoSTEP-EKAR
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3.1-nAChr-beta3

    Plasmid

    #48811

    Purpose

    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.

    Depositor

    Henry Lester, Jerry Stitzel

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    *

    Promoter

    CMV

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. a4 ecto pcDNA3.1 hygro(-)

    Plasmid

    #113406

    Purpose

    expression of human alpha 4 ectodomain with purification tags and C-terminal clasp

    Depositor

    Timothy Springer

    Article

    Li et al J Cell Biol. 2018 Jan 2;217(1):397-412.

    Insert

    human alpha 4 ectodomain
    

    Use

    Tags

    Strep II tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-CCND1(T286A)

    Plasmid

    #172631

    Purpose

    Expresses untagged cyclin D1(T286A) in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Thr286Ala

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. XE212 RGS9-pcDNA3.1

    Plasmid

    #16676

    Depositor

    Randall Moon

    Insert

    RGS9 (Rgs9 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1(+) ZKSCAN1 Antisense

    Plasmid

    #60632

    Purpose

    Negative Control for the pcDNA3.1(+) ZKSCAN1 Sense plasmid

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    ZKSCAN1 (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+) EPHB4 Antisense

    Plasmid

    #60640

    Purpose

    Negative Control for the pcDNA3.1(+) EPHB4 Sense plasmid

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    Ephrin type B receptor 4 (EPHB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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