We narrowed to 6,165 results for: cas9 expression plasmid

1. pET28b-SpRY-His

   Plasmid

   #179320

   Purpose

   Plasmid for bacterial expression and purification of SpRY-Cas9

   Depositor

   Miguel Angel Moreno-Mateos, Julián Cerón

   Article

   Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

   Insert

   Human codon-optimized Cas9 variant SpRY
   

   Use

   CRISPR

   Tags

   6xHis and SV40-NLS

   Expression

   Bacterial

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCRRNAcos

   Plasmid

   #78492

   Purpose

   Transcription of Streptococcus pyogenes crRNA and tracrRNA under the wildtype crRNA promoter.

   Depositor

   David Bikard

   Article

   Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

   Insert

   tracrRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HCP2

   Plasmid

   #166104

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HCP3

   Plasmid

   #166105

   Purpose

   This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination

   Depositor

   Matthias Heinemann

   Insert

   Msn2-sgRNA#27 (MSN2 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HCP7

   Plasmid

   #166109

   Purpose

   This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.

   Depositor

   Matthias Heinemann

   Insert

   Whi5-sg106 (WHI5 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pACRISPR

   Plasmid

   #113348

   Purpose

   A sgRNA expression plasmid for genome editing in Pseudomonas aeruginosa

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-BbsI-chiRNA

   Plasmid

   #45946

   Purpose

   Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

   Depositor

   Jill Wildonger, Kate O'Connor-Giles, Melissa Harrison

   Article

   Gratz et al Genetics. 2013 May 24.

   Insert

   U6-BbsI-chiRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Dm-snRNA:U6:96Ab

   Available since

   June 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. AD59_pEP.DonorCLYBL.TS

   Plasmid

   #199227

   Purpose

   Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   Expression unit for PuroR.T2A.EGFP selectable and reporter markers
   

   Use

   Gene targeting donor plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1 gene promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-MBP-NLS-Geo_st

   Plasmid

   #87703

   Purpose

   Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

   Depositor

   Jennifer Doudna

   Insert

   GeoCas9
   

   Use

   Tags

   10xHis-MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSBtet-RB-PE2

    Plasmid

    #173903

    Purpose

    SB-transposon with inducible expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pML107-KAE1

    Plasmid

    #232891

    Purpose

    Plasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    KAE1 gRNA (KAE1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pML107-SAM50

    Plasmid

    #232895

    Purpose

    Plasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    SAM50 gRNA (SAM50 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pML107-ARB1

    Plasmid

    #232888

    Purpose

    Plasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    ARB1 gRNA (ARB1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pML107-RER2

    Plasmid

    #232884

    Purpose

    Plasmid expressing Cas9 and gRNA AGAATCGCATCTCTACACGG which targets the RER2 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    RER2 gRNA (RER2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pML104-LEU2-3

    Plasmid

    #232894

    Purpose

    Plasmid expressing Cas9 and gRNA GTTCTTAACTAGGATCATGG which targets the RER2 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    LEU2 gRNA (RER2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pML104-HygMx4-URA3-3

    Plasmid

    #232886

    Purpose

    Plasmid expressing Cas9 and gRNA GAAGTAACAAAGGAACCTAG which targets the URA3 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    URA3 gRNA (URA3 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pML104-HygMx4-URA3-2

    Plasmid

    #232885

    Purpose

    Plasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    URA3 gRNA (URA3 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pML107-AVO1

    Plasmid

    #232889

    Purpose

    Plasmid expressing Cas9 and gRNA AATGATGACGACCATACCAG which targets the AVO1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    AVO1 gRNA (AVO1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pML107-YFR054C

    Plasmid

    #232900

    Purpose

    Plasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    YFR054C gRNA (YFR054C Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pML107-ZIM17

    Plasmid

    #232901

    Purpose

    Plasmid expressing Cas9 and gRNA AAATGTCTCACTTTGCAGTG which targets the ZIM17 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    ZIM17 gRNA (ZIM17 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pML107-SRP14

    Plasmid

    #232896

    Purpose

    Plasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    SRP14 gRNA (SRP14 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pML107-TLG1

    Plasmid

    #232897

    Purpose

    Plasmid expressing Cas9 and gRNA GATGAAAACGAAGACGTGAG which targets the TLG1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    TLG1 gRNA (TLG1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pML107-VHT1

    Plasmid

    #232898

    Purpose

    Plasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    VHT1 gRNA (VHT1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pML107-ADE13

    Plasmid

    #232887

    Purpose

    Plasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    ADE13 gRNA (ADE13 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pML107-LAS17

    Plasmid

    #232892

    Purpose

    Plasmid expressing Cas9 and gRNA AATACCCTGAATTCTGCCGG which targets the LAS17 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    LAS17 gRNA (LAS17 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pML104-LEU2-2

    Plasmid

    #232883

    Purpose

    Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    LEU2 gRNA (LEU2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pML107-INO80

    Plasmid

    #232890

    Purpose

    Plasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    INO80 gRNA (INO80 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSBbi-RB-PE2

    Plasmid

    #173902

    Purpose

    SB-transposon with constitutive expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. BB3nK_ext_AC

    Plasmid

    #108686

    Purpose

    Plasmid for assembly of donor DNA templates consisting of two expression units

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. BB3nK_ext_AD

    Plasmid

    #108687

    Purpose

    Plasmid for assembly of donor DNA templates consisting of three expression units

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge