1,878 results

1. HRP-1LC

   Plasmid

   #139116

   Purpose

   express the LC of HRP-1

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Expression

   Bacterial

   Mutation

   181-346

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJ4M_HRP-1_FL

   Plasmid

   #139117

   Purpose

   express HRP-1 with a C-terminal maltose binding protein tag

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hrp-1 (hrp-1 Nematode)
   

   Expression

   Bacterial

   Mutation

   None

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

   Plasmid

   #159838

   Purpose

   Three-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHJ4

   Plasmid

   #158785

   Purpose

   Pan-neuronal nuclear-localized GCaMP expression in C. elegans

   Depositor

   Zachary Knight, Jennifer Garrison

   Article

   Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

   Insert

   Prab-3::nls::GCaMP6m
   

   Tags

   NLS

   Expression

   Worm

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJW1884

   Plasmid

   #154332

   Purpose

   sgRNA Target Vector for CRISPR

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   cxTi10882 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJW1827

   Plasmid

   #154323

   Purpose

   Multi-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mScarlet-I
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJW1841

   Plasmid

   #154338

   Purpose

   Promoter reporter

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR
   

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJW1948

   Plasmid

   #154340

   Purpose

   Enhancer reporter

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   pes-10delta::SV40 NLS::mScarlet_I::PEST(dpi)::-tbb-2 3'UTR
   

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDD289

   Plasmid

   #137018

   Purpose

   GFP-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein, Daniel Dickinson

   Insert

   GFP-GLO^SEC^3xFlag + ccdB
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans GLO-optimized GFP

   Expression

   Worm

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJW4.34

    Plasmid

    #131370

    Purpose

    expression of GST:MEG-4 in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    meg-4 (C36C9.1 Nematode)
    

    Expression

    Bacterial

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDD376

    Plasmid

    #137022

    Purpose

    SapTrap mKate2-GLO donor

    Depositor

    Bob Goldstein, Daniel Dickinson

    Insert

    C. elegans GLO-optimized mKate2
    

    Use

    CRISPR; Saptrap donor for cloning repair templat…

    Tags

    C. elegans GLO-optimized mKate2

    Expression

    Worm

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXF90

    Plasmid

    #139044

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    halotag::HDEL (includes STOP codon, PATC introns)
    

    Expression

    Worm

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDK167

    Plasmid

    #134188

    Purpose

    Expression of C. elegans KNL1

    Depositor

    Iain Cheeseman

    Article

    Kern et al Mol Biol Cell. 2015 Jan 15;26(2):229-37.

    Insert

    KNL1 FL with oligomutant (knl-1 Nematode)
    

    Expression

    Bacterial

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDK540

    Plasmid

    #134201

    Purpose

    Bacterial expression of C. elegans KNL1

    Depositor

    Iain Cheeseman

    Article

    Kern et al Mol Biol Cell. 2015 Jan 15;26(2):229-37.

    Insert

    KNL1 FL with YA mutant (knl-1 Nematode)
    

    Expression

    Bacterial

    Mutation

    YA

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDK134

    Plasmid

    #134187

    Purpose

    Expression of C. elegans KNL1

    Depositor

    Iain Cheeseman

    Article

    Kern et al Mol Biol Cell. 2015 Jan 15;26(2):229-37.

    Insert

    KNL1 aa 69 - 479 (knl-1 Nematode)
    

    Expression

    Bacterial

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDK324

    Plasmid

    #134190

    Purpose

    Expression of C. elegans KNL1 with 8A mutation

    Depositor

    Iain Cheeseman

    Insert

    KNL1 (knl-1 Nematode)
    

    Expression

    Bacterial

    Mutation

    8A

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDK038

    Plasmid

    #134182

    Purpose

    Expression of C. elegans KNL1

    Depositor

    Iain Cheeseman

    Article

    Kern et al Mol Biol Cell. 2015 Jan 15;26(2):229-37.

    Insert

    KNL1 aa 69-235 (knl-1 Nematode)
    

    Expression

    Bacterial

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJS263

    Plasmid

    #134141

    Purpose

    Bacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    Ska1 MTBD-His (Y106G6H.15 Nematode)
    

    Expression

    Bacterial

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCZGY921

    Plasmid

    #135092

    Purpose

    Transcriptional GFP reporter of acr-2 (cholinergic) expressing neurons in C. elegans

    Depositor

    Yishi Jin

    Article

    Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

    Insert

    Pacr-2
    

    Tags

    GFP and Synthetic Intron

    Expression

    Worm

    Promoter

    Pacr-2

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCZGY2750

    Plasmid

    #135094

    Purpose

    Site specific CRISPR/Cas9 editing of C. elegans Chr IV

    Depositor

    Yishi Jin

    Article

    Andrusiak et al Neuron. 2019 Jul 24. pii: S0896-6273(19)

    Insert

    sgRNA for cxTi10082 (actgttggatgcctgtgtag)
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. Myo 9 Avi+Flag

    Plasmid

    #134969

    Purpose

    Expression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1

    Depositor

    Martin Bähler

    Article

    Liao et al J Biol Chem. 2010 Aug 6;285(32):24933-42

    Insert

    Myo 9 (hum-7 Nematode)
    

    Tags

    Avi and Flag

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pJW4.33

    Plasmid

    #131369

    Purpose

    expression of GST:MEG-3 in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    meg-3 (meg-3 Nematode)
    

    Expression

    Bacterial

    Available Since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Unc104_K560GFP

    Plasmid

    #129773

    Purpose

    Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged

    Depositor

    William Hancock

    Insert

    Unc104 (unc-104 Nematode)
    

    Tags

    GFP and His6

    Expression

    Bacterial

    Mutation

    Truncation at 361, fused to kinesin-1 starting Al…

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pJW4.15

    Plasmid

    #131373

    Purpose

    expression of MBP:PAA-1 in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    paa-1 (paa-1 Nematode)
    

    Expression

    Bacterial

    Available Since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJW4.31

    Plasmid

    #131371

    Purpose

    expression of GST:MEG-1 in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    meg-1 (meg-1 Nematode)
    

    Expression

    Bacterial

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJW4.13

    Plasmid

    #131375

    Purpose

    expression of MBP:PPTR-2 in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    pptr-2 (pptr-2 Nematode)
    

    Expression

    Bacterial

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJW4.12

    Plasmid

    #131374

    Purpose

    expression of MBP:PPTR-1 in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    pptr-1 (pptr-1 Nematode)
    

    Expression

    Bacterial

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDC24

    Plasmid

    #131368

    Purpose

    expression of MEG-3Cterm (aa545-862) in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

    Insert

    meg-3 (aa545-862) (meg-3 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDC21

    Plasmid

    #131367

    Purpose

    expression of MEG-3IDR (aa1-544) in C. elegans

    Depositor

    Geraldine Seydoux

    Article

    Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

    Insert

    meg-3 (aa1-544) (meg-3 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJPL3[flp-6p::cmk-1::GFP]

    Plasmid

    #124613

    Purpose

    Used to confirm the expression of the trans-spliced GFP reporter in the expected ASE neurons

    Depositor

    Anne Brunet

    Article

    Lim et al J Neurosci. 2018 Jul 4;38(27):6114-6129.

    Insert

    [flp-6p::cmk-1::GFP]
    

    Expression

    Worm

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge