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Showing: 2041 - 2080 of 27024 results

  1. pET-29b(+)_His-CBD-SpEndoH

    Plasmid
    #218196
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH
    Depositor
    Florence Pojer
    Insert
    EndoH
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 45-313
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218196-plasmid-map-sequence-id-431799

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  3. bU6-sgMark2-mU6-sgMark3-hU6-sgMark4

    Plasmid
    #177235
    Purpose
    Expresses Mark2 (bU6), Mark3 (mU6) and Mark4 (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgMark2/sgMark3/sgMark4
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    177235-plasmid-map-sequence-id-352696

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  6. pET28a-mOct4 Full length(FL)

    Plasmid
    #206394
    Purpose
    Expresses Oct4 with a 6xHis tag for protein purification
    Depositor
    Ralf Jauch
    Insert
    Oct4 (Pou5f1 Mouse)
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    206394-plasmid-map-sequence-id-409984

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  7. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Nathan Reyna
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68374-plasmid-map-sequence-id-121945

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  10. pOliSpec5'

    Plasmid
    #228551
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228551-plasmid-map-sequence-id-454860

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  11. pOliSpec3'

    Plasmid
    #228550
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228550-plasmid-map-sequence-id-454859

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  13. Sc RFC[hk-1s + 5]-pLANT/RIL

    Plasmid
    #239200
    Purpose
    Overeexpress Sc RFC1 (truncated N-terminus; N-terminal his tag, PKA kinase motif) in E. coli
    Depositor
    Michael O'Donnell
    Inserts
    RFC1 (RFC1 Budding Yeast)
    RFC5 (RFC5 Budding Yeast)
    Tags
    His-10 + PKA kinase motif on truncated Sc RFC1
    Expression
    Bacterial
    Mutation
    N-terminal truncation
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239200-plasmid-map-sequence-id-476583

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  16. pLSU1/t35s[]:mCherry-I{PIV-180}:NosT

    Plasmid
    #212179
    Purpose
    This binary vector expresses mCherry containing the PIV intron (180bp into the gene) with the truncated 35sCAMV promoter
    Depositor
    Wayne Curtis
    Insert
    mCherry-I{PIV-180}
    Expression
    Plant
    Mutation
    Truncated mCherry, missing the first 9 amino acid…
    Promoter
    truncated 35sCaMV
    Available Since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212179-plasmid-map-sequence-id-443888

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  18. pOpen-RNAse A

    Plasmid
    #165565
    Purpose
    Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    RNAse A
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165565-plasmid-map-sequence-id-340093

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  19. pOCC102-His-MBP-DDX3X(WT)

    Plasmid
    #219988
    Purpose
    DDX3X(WT) protein purification
    Depositor
    Simon Alberti
    Insert
    DDX3X(WT) (DDX3X Human)
    Tags
    6xHis and MBP
    Expression
    Insect
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219988-plasmid-map-sequence-id-436790

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  26. pTXB1-sfGFP

    Plasmid
    #51562
    Purpose
    Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    superfolder GFP
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Available Since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51562-plasmid-map-sequence-id-78800

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  29. pOCC120-MBP-DDX3X-monoGFP-His

    Plasmid
    #219985
    Purpose
    DDX3X(WT) protein purification
    Depositor
    Simon Alberti
    Insert
    DDX3X(WT) (DDX3X Human)
    Tags
    MBP and monoGFP and 6xHis
    Expression
    Insect
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219985-plasmid-map-sequence-id-436890

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  31. pRAV12

    Plasmid
    #67713
    Purpose
    Parental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purification
    Depositor
    Robert Batey, Jeffrey Kieft
    Article
    Kieft et al RNA. 2004 Jun;10(6):988-95.
    Insert
    Transcription cassette for 3'-tagged RNAs
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7 RNA polymerase
    Available Since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    67713-plasmid-map-sequence-id-126588

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  34. lentiGuideB3GNT5-neo

    Plasmid
    #220867
    Purpose
    encodes CRISPR sgRNA targeting human B3GNT5
    Depositor
    Jennifer Kohler
    Insert
    B3GNT5 (B3GNT5 Human)
    Use
    Lentiviral
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220867-plasmid-map-sequence-id-440866

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  35. pLSU1/t35s[TMV]:mNeonGreen-I{PDK-198}:NosT

    Plasmid
    #212170
    Purpose
    This binary vector expresses mNeonGreen containing the castor bean catalase (PDK) intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR
    Depositor
    Wayne Curtis
    Insert
    Tomato codon optimized version of mNeonGreen-I{PDK-198}
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PDK int…
    Promoter
    truncated 35sCaMV
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212170-plasmid-map-sequence-id-422699

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  39. rEstus

    Plasmid
    #209767
    Purpose
    To express a bright and sensitive genetically encoded voltage indicator in mammalian cells.
    Depositor
    Stefan Heinemann
    Article
    Rühl et al bioRxiv 2023.
    Insert
    rEstus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    209767-plasmid-map-sequence-id-413323

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  40. RHCglo

    Plasmid
    #80169
    Purpose
    Minigene reporter for identification and analysis of cis elements and trans factors affecting pre-mRNA splicing
    Depositor
    Thomas Cooper
    Article
    Singh et al Biotechniques. 2006 Aug;41(2):177-81.
    Insert
    segments of chicken cardiac troponin T (Tnnt2) introns 4 and 5 flanking an artifical exon
    Expression
    Mammalian
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80169-plasmid-map-sequence-id-150808

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Showing: 2041 - 2080 of 27024 results