We narrowed to 1,229 results for: Aspa

Showing: 1021 - 1040 of 1229 results

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pcDNA3.3−3xFLAG−AGO2(S387D)

Plasmid

#220463

Purpose

Express S387 phosphomimetic mutant human AGO2 with N-terminal 3xFLAG tag in mammalian cells

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

Human AGO2 (AGO2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Serine 387 to aspartate

Promoter

CMV

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pYFP-NGAT(A193T,N194Y)

Plasmid

#11388

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Changed Alanine 193 to Threonine, and Asparagine …

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3T911A

Plasmid

#120254

Purpose

Expression of ATP3-6 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Expression

Yeast

Mutation

T911 changed to A; Isoleucine 304 changed to Aspa…

Promoter

ATP3

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-mTDGa.1

Plasmid

#81051

Purpose

bacterial expression of catalytically dead murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

asparagine 151 changed to alanine

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pMALp2x-SCO5461(E164D)(43-204)

Plasmid

#42508

Depositor

Koji Okamoto

Article

Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

Insert

guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))

Tags

maltose-binding protein

Expression

Bacterial

Mutation

Deleted amino acids 1-42 (nucleotide 1-126); chan…

Promoter

tac

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pTG-mTDGa.1

Plasmid

#81049

Purpose

bacterial expression of catalytically dead murine TDG

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TDG (Tdg Mouse)

Tags

GST

Expression

Bacterial

Mutation

asparagine 151 changed to alanine

Promoter

T7

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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CITED2-T166N

Plasmid

#47749

Purpose

Introduces a T166N point mutation in the CITED2 gene

Depositor

Shoumo Bhattacharya

Article

Chen et al PLoS One. 2012;7(10):e46256. doi: 10.137

Insert

CITED2 (CITED2 Human)

Expression

Mammalian

Mutation

Threonine 166 to Asparagine

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
DDT Wnt

Plasmid

#248843

Purpose

Double Death Trap (DDT) Wnt reporter Containing FKBP12(F36V)-ΔCaspase9 (FC) and Puromycin Resistance Gene for Assessing Wnt Signaling

Depositor

Hai Jiang

Article

He et al Cell Chem Biol. 2025 Oct 22:S2451-9456(2

Insert

7xTCF binding element

Use

Lentiviral

Promoter

7xTCF binding element & minimal TATA-box prom…

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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Shim_AspRS_3212.91_TP_GFP_pK7fWG2_IVD_FP611

Plasmid

#228374

Purpose

Expresses N-term transit peptide from Sapria himalayana Aspartate aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 3212.91; determination of S. himalayana AspRS target localization

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from S. himalayana AspRS evm.model.fragScaff scaffold 3212.91

Tags

Green Florescent Protien

Expression

Plant

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-parkin K161N

Plasmid

#227365

Purpose

For bacterial expression of parkin with a mutated pUbl/pUb binding site on RING0.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin K161N (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC8 D101R

Plasmid

#224349

Purpose

Expresses human KDAC8 (HDAC8) D101R in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to arginine

Promoter

T5

Available Since

Oct. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJExpress401-KDAC8 D101A

Plasmid

#224347

Purpose

Expresses human KDAC8 (HDAC8) D101A in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to alanine

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P

Plasmid

#216404

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/N102/Q443 to proline mutations

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-A51P/A56P/Q70P/N102P/Q443P

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Alanine 51, 56, Glutamine 70, 443 and Asp…

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[V458D][Q459D][V460D][G461D]-FLAG

Plasmid

#199327

Purpose

mutated RHIM motive (interaction active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed V458, Q459,V460 and G461 to aspartic acid

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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DNMT2Δ191-237

Plasmid

#198382

Purpose

Bacterial expression plasmid for human DNMT2 deletion mutant; for binding assays

Depositor

Tanja Schirmeister

Article

Schwickert et al ACS Med Chem Lett. 2023 May 9;14(6):777-

Insert

DNMT2 (TRDMT1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

deleted amino acids 191-237

Promoter

T7

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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Sandgrouse+R111Q–insD

Plasmid

#191229

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

removed aspartic acid at 121 and replaced arginin…

Promoter

PH and p10

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pRS313_ATP3mut

Plasmid

#120256

Purpose

Expression of ATP3-67 in yeast

Depositor

Markus Ralser

Article

Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

Insert

ATP3 (ATP3 Budding Yeast)

Expression

Yeast

Mutation

Isoleucine 304 changed to Asparagine; Alanine 307…

Promoter

ATP3

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) R104D

Plasmid

#125827

Purpose

Expresses GST-RPN13 (19-132) R104D in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed arginine 104 to aspartate

Promoter

T7

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-GFP-Atg17(416) (L313D I314K)

Plasmid

#121409

Purpose

Expressing GFP-Atg17

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

CUP1p-GFP-ATG17 ORF-CYC1 terminator

Expression

Yeast

Mutation

Leucine 313 to Aspartic acid; Isoleucine 314 to …

Promoter

CUP1 promoter

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]

Plasmid

#106462

Purpose

Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(N163,242Q)

Tags

PA

Expression

Bacterial and Yeast

Mutation

changed Asparagines at positions 163 and 242 to G…

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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