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Showing: 1021 - 1040 of 13280 results

  1. CAGGS-SAFB-DD3-NLS-3XFLAG-V5

    Plasmid
    #196095
    Purpose
    Expresses only the third computationally called disordered domain of mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag
    Depositor
    Mauro Calabrese
    Article
    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10
    Insert
    SAFB (Safb Mouse)
    Use
    Tags
    C-terminal 3xFLAG and C-terminal V5
    Expression
    Bacterial and Mammalian
    Mutation
    Contains only AA 619-925 (NLS and predicted disor…
    Promoter
    CAGGS
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196095-plasmid-map-sequence-id-384632

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  2. pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid
    #113701
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113701-plasmid-map-sequence-id-232036

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  3. pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid
    #113699
    Purpose
    Dox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113699-plasmid-map-sequence-id-229551

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  10. pcDNA3-ps-CFP

    Plasmid
    #13034
    Purpose
    Expresses photo-switchable CFP in mammalian cells. Can undergo irreversible photoconversion from cyan to green fluorescence
    Depositor
    Doug Golenbock
    Insert
    photo-switchable cyan fluorescent protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    13034-plasmid-map-sequence-id-7430

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  11. Hsp68-LacZ-Gibson

    Plasmid
    #170102
    Purpose
    Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.
    Depositor
    Len Pennacchio
    Type
    Empty backbone
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Mouse Hsp68 minimal promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170102-plasmid-map-sequence-id-346308

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  12. pGHE-PdNIP2-1

    Plasmid
    #108447
    Purpose
    PdNIP2-1 gene from Date Palm (Phoenix dactylifera) cloned in pGHE. Can be used for Xenopus oocyte assay. The vector has Xenopus globin 3UTR and 5UTR, pT7, and polyA.
    Depositor
    Richard Bélanger
    Insert
    PdNIP2-1
    Use
    Tags
    Expression
    Mutation
    Promoter
    pT7
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    108447-plasmid-map-sequence-id-274297

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  13. RV-Syn-Dio-mVenus

    Plasmid
    #153204
    Purpose
    Can be used to generate retrovirus that will mark the membrane (Palmitoylated-Venus) in the presence of Cre from the rat synapsin promoter
    Depositor
    Lynette Lim
    Insert
    membrane-tagged-Venus
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    rat synapsin
    Available since
    June 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    153204-plasmid-map-sequence-id-298905

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  14. pANY4-pL18-ccdB

    Plasmid
    #164898
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164898-plasmid-map-sequence-id-327989

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  15. RV-Cag-Dio-mVenus

    Plasmid
    #153202
    Purpose
    Can be used to generate retrovirus that will mark the membrane (Palmitoylated-Venus) in the presence of Cre from the CAG promoter
    Depositor
    Lynette Lim
    Insert
    membrane-tagged-Venus
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    CAG
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    153202-plasmid-map-sequence-id-305047

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  16. pT7TS-rich

    Plasmid
    #99050
    Purpose
    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.
    Depositor
    Richard Bélanger
    Type
    Empty backbone
    Use
    Unspecified; Xenopus oocyte system
    Tags
    Expression
    Mutation
    Promoter
    pT7
    Available since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    99050-plasmid-map-sequence-id-191760

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  17. INT_Cargo_PgyrA_gfp

    Plasmid
    #212138
    Purpose
    Cargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212138-plasmid-map-sequence-id-420768

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  18. pRS415 GPDpro-RNQ1-CFP

    Plasmid
    #224887
    Purpose
    Low copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).
    Depositor
    Paul Herman
    Insert
    RNQ1 (RNQ1 Budding Yeast)
    Use
    Tags
    CFP
    Expression
    Yeast
    Mutation
    Promoter
    GPD
    Available since
    Oct. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    224887-plasmid-map-sequence-id-448181

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  19. pAG1guide_RUBY_EggCas9

    Plasmid
    #225983
    Purpose
    To make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plants
    Depositor
    Imran Siddiqi
    Insert
    Guide RNA against AtAGAMOUS1
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35s promoter to Drive RUBY and DD45p to drive Cas9
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225983-plasmid-map-sequence-id-448020

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  20. INT_Cargo_T7_gutB

    Plasmid
    #212139
    Purpose
    Cargo plasmid for integrating PT7 expression cassette of xylitol dehydrogenase in in genome.Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    xylitol dehydrogenase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    212139-plasmid-map-sequence-id-424777

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Showing: 1021 - 1040 of 13280 results