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pCMV-Tag/WT M1

Plasmid

#89321

Purpose

expression of M1 C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted

Promoter

CMV

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M1C448Y

Plasmid

#89319

Purpose

expression of M1 C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, C448Y

Promoter

CMV

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mTFP-NLS-blast

Plasmid

#136257

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mTFP-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pABM-D4-miR30-L1221

Plasmid

#132933

Purpose

Lentivector for luciferase (control) knockdown and blasticidin resistance

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

luciferase shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M87 C448Y

Plasmid

#89320

Purpose

expression of M87 C448Y spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-460 bp deleted

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pABM-D4-miR30-CypA

Plasmid

#132934

Purpose

Lentivector for CypA knockdown and blasticidin resistance

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

CypA shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pDest_pK7WG2_pRPS5A_CTP OleGFP

Plasmid

#171723

Purpose

ptpTALECD vector assembly, Destination vector;

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

RPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMM532

Plasmid

#112529

Purpose

pZA1D-hrtR - HrtR expressed consitutively from promoter ProD with RBS2, p15a origin, AmpR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Insert

HrtR

Use

Tags

Expression

Bacterial

Mutation

Promoter

ProD

Available since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1WT

Plasmid

#89327

Purpose

Inducible expression of WT M1 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted

Promoter

Tet-responsive P tight

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87 WT

Plasmid

#89328

Purpose

Inducible expression of WT M87 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-460 bp deleted

Promoter

Tet-responsive P tight

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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UAS::PTPRDb-DN-GFP

Plasmid

#37112

Depositor

Alvaro Sagasti

Article

Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

Insert

UAS::PTPRDb-DN-GFP (ptprdb Zebrafish)

Use

Tol2 gateway destination vector

Tags

EGFP

Expression

Mutation

deleted cytodomain, including phosphatase domains…

Promoter

UAS

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pAGH51

Plasmid

#107255

Purpose

HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.

Depositor

Erin O'Shea

Article

Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

Insert

HU-FRB

Use

Synthetic Biology

Tags

FRB

Expression

Mutation

Promoter

native HU promoter

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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CpcB•PHLS+Cpc

Plasmid

#73335

Purpose

Expresses the PHLS gene as a fusion with the CpcB gene followed by the CmR gene

Depositor

Anastasios Melis

Article

Formighieri et al Metab Eng. 2015 Nov;32:116-24. doi: 10.1

Insert

cpcB•PHLS-CmR

Use

Tags

Expression

Mutation

Promoter

cpc operon

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pVEA3-DR

Plasmid

#234800

Purpose

Mammalian expression of bovine arrestin-3 (D291R)

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Use

Tags

Venus

Expression

Mammalian

Mutation

D291R

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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p3VEA3

Plasmid

#234796

Purpose

Mammalian expression of bovine arrestin-3

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Use

Tags

Venus

Expression

Mammalian

Mutation

none

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVEA3-Tr

Plasmid

#234798

Purpose

Mammalian expression of truncated bovine arrestin-3 (1-393)

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Use

Tags

Venus

Expression

Mammalian

Mutation

truncated (1-393)

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pVEA3-RE

Plasmid

#234799

Purpose

Mammalian expression of bovine arrestin-3 (R170E)

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Use

Tags

Venus

Expression

Mammalian

Mutation

R170E

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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TC alphaDdCBE T2

Plasmid

#221991

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-TC TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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TL1 DdCBE A1 (DddA6-C)

Plasmid

#221992

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

DddA: T1413I

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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TL1 DdCBE T2 (DddA6-N)

Plasmid

#221993

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

DddA: Q1310R, S1330I, T1380I

Promoter

CMV

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge