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We narrowed to 2,542 results for: pcas9

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Showing: 1021 - 1040 of 2542 results

  1. Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)

    Plasmid
    #217797
    Purpose
    Variant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Cdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Cdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217797-plasmid-map-sequence-id-432864

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  2. pCMV-PEmax-P2A-hMLH1dn

    Plasmid
    #174828
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Insert
    PEmax-P2A-hMLH1dn
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript; deletion of residues 754-…
    Promoter
    CMV
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174828-plasmid-map-sequence-id-357934

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  3. C-term AAV-eVRQR-ACTA2-gRNA-A4 (mouse) (LLH310)

    Plasmid
    #242662
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and mouse gRNA-A4
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_mouse_NGA_gRNA-pU6
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242662-plasmid-map-sequence-id-489091

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  4. C-term AAV-eVRQR-ACTA2-gRNA-A4 (human) (LLH301)

    Plasmid
    #242661
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and human gRNA-A4
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_human_NGA_gRNA-pU6
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242661-plasmid-map-sequence-id-489090

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  5. pCMV-T7 RNA POL

    Plasmid
    #138352
    Purpose
    Expressing T7 RNA Polymerase in mammalian cells
    Depositor
    Baisong Lu
    Insert
    T7 RNA POL
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    138352-plasmid-map-sequence-id-273271

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  6. pCMV-PE2-pegRNA-tetra-com vector

    Plasmid
    #136271
    Purpose
    Expresses PE2 and Tetra-com modified pegRNA cassette
    Depositor
    Baisong Lu
    Insert
    PE2 and pegRNA cassette
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    136271-plasmid-map-sequence-id-273274

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  7. pCMV-PE2-pegRNA-ST2-com-vector

    Plasmid
    #136272
    Purpose
    Expresses PE2 and ST2-com modified pegRNA cassette
    Depositor
    Baisong Lu
    Insert
    PE2, pegRNA scaffold
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    136272-plasmid-map-sequence-id-273277

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  9. Cas9_P2A_POLB

    Plasmid
    #190145
    Purpose
    Expresses SpCas9 and POLB
    Depositor
    Timothy Lu
    Article
    Nakade et al bioRxiv 2022.12.05.518807
    Insert
    SpCas9_P2A_POLB
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Codon Optimized for mammalian cells
    Promoter
    CBh
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    190145-plasmid-map-sequence-id-376721

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  10. LP102: pMAGIC (R4-R3) NLS-Sp Cas9-NLS

    Plasmid
    #132927
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2xNLS SpCas9 (nuclease active) for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Insert
    humanized SpCas9
    Use
    CRISPR, Gateway Cloning, and Synthetic Biology
    Available Since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    132927-plasmid-map-sequence-id-314451

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Showing: 1021 - 1040 of 2542 results