We narrowed to 13,228 results for: sequence
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Plasmid#61387PurposemRNA synthesis of bacterial nitroreductase NfsB codon modified for zebrafishDepositorInsertnfsB.zf1
UseZebrafish expressionMutationE28GPromotersp6Available SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBlueScript-humanAPOBEC1
Plasmid#134606PurposePlasmid bearing APOBEC1 coding sequence. In presence of its cofactor and overexpressing an artificial reporter with editable CAA codon in APOB sequence we can visualize its editing activityDepositorArticleAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-TagRFPT.zf1
Plasmid#61390PurposemRNA synthesis of TagRFPT codon modified for zebrafishDepositorInsertTagRFPT.zf1
UseZebrafish expressionPromotersp6Available SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-IE1
Plasmid#52894Purposebaculovirus IE-1 promoter driving firefly luciferaseDepositorInsertIE1 promoter
UseLuciferasePromoterbaculovirus IE1Available SinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC_sgRNA
Plasmid#68710PurposeContains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyAvailable SinceSept. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAP24
Plasmid#70190PurposeModular vector for secreted luciferase reporter expressionDepositorInsertsLucopt
UseSynthetic BiologyExpressionBacterialPromoterPtetAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pENTR2B-dCas9-KRAB
Plasmid#115547PurposeEntry cassette for dCas9-KRABDepositorInsertdCas9-KRAB
TagsHAExpressionBacterial and MammalianAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-2xMARS-nGFP-puro
Plasmid#205239PurposeDOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon systemDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseTransposonTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…Promotertight TRE promoterAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3-MARS-p85(iSH2)
Plasmid#205236PurposeExpresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85betaDepositorTagsNuclear Export Sequence and mCherryExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cer.zf1
Plasmid#61389PurposemRNA synthesis of cerulean codon modified for zebrafishDepositorInsertCer.zf1
UseZebrafish expressionPromotersp6Available SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHisIHFαIHFβ
Plasmid#149384PurposeExpression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbetaDepositorInsertsPseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
Pseudomonas aeruginosa PA14 IHFbeta
Tags6xHis tag and HRV3C cleavage siteExpressionBacterialPromoterT7Available SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStrepIHFb
Plasmid#149385PurposeExpression of P. aeruginosa PA14, StrepII-HRV3C-IHFbetaDepositorInsertPseudomonas aeruginosa PA14 StrepII-tagged IHFbeta
TagsHRV3C cleavage site and StrepII-tag withExpressionBacterialPromoterT7Available SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
plIVMD156
Plasmid#160934PurposeIn vivo mRNA display Gateway Destination constructDepositorInsertMCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
TagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastPromoterMET25Available SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
1.6 kB VEGF-luc
Plasmid#29667DepositorInsert1.6 kB VEGF
UseLuciferaseAvailable SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pWKS1583
Plasmid#70188PurposeModular vector for secreted amylase reporter expressionDepositorInsertamyEopt
UseSynthetic BiologyExpressionBacterialPromoterPtetAvailable SinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -