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Showing: 10381 - 10400 of 18163 results

  5. Top7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L

    Plasmid
    #12474
    Depositor
    David Baker
    Insert
    Top7 N7K D9T D10V N14T F15Y D16E Y17I Y37L K39E K40E Q41L
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    b19 only. N7K D9T D10V N14T F15Y D16E Y17I Y37L K…
    Available Since
    March 13, 2007
    Availability
    Academic Institutions and Nonprofits only

  6. pLANT-2b

    Plasmid
    #231487
    Purpose
    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.
    Depositor
    Venkat Gopalan
    Inserts
    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    Tags
    His6 tag and His6-SUMO^NIT
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…
    Promoter
    T7 promoter
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231487-plasmid-map-sequence-id-460673

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  8. pSBtet-2xMARS-nGFP-puro

    Plasmid
    #205239
    Purpose
    DOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon system
    Depositor
    Jeremy Baskin
    Article
    Cao et al Nat Cell Biol. 2024 Oct;26(10):1804-1816
    Insert
    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    Use
    Transposon
    Tags
    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    tight TRE promoter
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205239-plasmid-map-sequence-id-404740

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  9. pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(MIN)

    Plasmid
    #204695
    Purpose
    This plasmid allows for inducible expression of BRD4-NUT(MIN) mutant fusion tagged with mNeonGreen, in mammalian cells.
    Depositor
    Michael Rosen
    Article
    Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1
    Insert
    BRD4-NUT(MIN) (BRD4, NUTM1 Human)
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    deletion of majority of NUT, retaining only amino…
    Promoter
    CMV
    Available Since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204695-plasmid-map-sequence-id-405171

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  12. pCRISPaint-mNeon-F-gB-PuroR [M1G]

    Plasmid
    #171803
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-gB-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available Since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171803-plasmid-map-sequence-id-341955

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  13. pCRISPaint-mScarlet-F-hgp100-PuroR [M1G]

    Plasmid
    #171810
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-hgp100-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171810-plasmid-map-sequence-id-337982

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  14. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

    Plasmid
    #171802
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-mgp100-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171802-plasmid-map-sequence-id-337973

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  16. pcDNA5/FRT/TO/Flag-mKO2-HuR.DelHNS

    Plasmid
    #110373
    Purpose
    Mammalian expression of mKO2-HuR (DelHNS) with FLAG tag, Flp-In T-Rex system
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Tags
    FLAG and mKO2
    Expression
    Mammalian
    Mutation
    HNS (HuR nuclear-cytoplasmic shuttling sequence) …
    Promoter
    CMV
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110373-plasmid-map-sequence-id-213400

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  17. pBEST-p70a-UTR1-deGFP Y35TAG-6xHis-T500

    Plasmid
    #92225
    Purpose
    Expression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    deGFP Y35TAG
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Residue Y35 mutated into TAG (Amber) stop codon f…
    Promoter
    p70a
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    92225-plasmid-map-sequence-id-197052

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  18. pET151-Vps4(101-437)-Hcp1 K321A/R325A

    Plasmid
    #89287
    Purpose
    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1
    Depositor
    Chris Hill
    Article
    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1
    Insert
    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    Tags
    6xHis-V5-TEV and Hcp1
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-100, mutated Lys-321 and Ar…
    Promoter
    T7
    Available Since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89287-plasmid-map-sequence-id-175359

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  20. pSH-hPXR-LBD-T248E

    Plasmid
    #41619
    Depositor
    Sridhar Mani
    Insert
    hPXR-LBD-T248E (NR1I2 Human)
    Expression
    Mammalian
    Mutation
    contains human PXR ligand binding domain (LBD, aa…
    Promoter
    pEN1 (chimeric HIV-1 LTR enhancer and HTLV-I LTR …
    Available Since
    April 24, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 10381 - 10400 of 18163 results