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pEB1-mCerulean

Plasmid

#103968

Purpose

Plasmid encoding mCerulean

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCerulean

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

ProC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET-Ran(Q69L)

Plasmid

#42048

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

Ran (RAN Human)

Tags

His

Expression

Bacterial

Mutation

Q69L

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-RFP-p2a-postASAP

Plasmid

#178793

Purpose

Expresses voltage sensor targeted to dendrites and spines along with RFP

Depositor

Rafael Yuste

Article

Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

Insert

FingR.PSD95, ASAP2f and TagRFP

Tags

TagRFP

Expression

Mammalian

Mutation

mutations in amino acids L146G, S147T N149R, S150…

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-mCardinal-CAXX

Plasmid

#184451

Purpose

Plasma membrane-targeted mCardinal expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

mCardinal-CAAX

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pmRuby3-24xGCN4

Plasmid

#132413

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

mRuby3

Expression

Mammalian

Mutation

no

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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p177bp-LacOx50-Hyg

Plasmid

#210213

Purpose

Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMVV052

Plasmid

#215303

Purpose

Expresses sfGFP under rhamnose induction (RhaS + RhaR + pRhaB-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PRhaB

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTlpA39-Wasabi

Plasmid

#86116

Purpose

mWasabi reporter gene gated by TlpA39

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA39
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A, D135V, A217V, L236F

Promoter

pTlpA and pTlpA, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBES1-LacOx50-Hyg

Plasmid

#210261

Purpose

Integrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHO4d-Cas9

Plasmid

#67881

Purpose

Cas9nlsHis6 Bacterial Expression construct

Depositor

Michael Nonet

Article

Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

Insert

Cas9

Use

CRISPR

Tags

His6 and T7 epitope

Expression

Bacterial

Promoter

T7

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pAR-Ec318

Plasmid

#130871

Purpose

A nuclear plasmid encoding the WT TP-DNAP1 for OrthoRep

Depositor

Chang Liu

Article

Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

Insert

TP-DNAP1

Expression

Yeast

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pHES553 [prGal1-RAS2(KAN) pnh604]

Plasmid

#103846

Purpose

Yeast Expression Plasmid

Depositor

Hana El-Samad

Article

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

pGal1-Ras2(KAN)

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-GFP

Plasmid

#83375

Purpose

Expression of Myc tagged GFP

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

Myc-GFP

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-30)

Plasmid

#199167

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(-30)

Tags

MGHHHHHHGGA

Mutation

T19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pVITRO1-iRFP702-LaminB1

Plasmid

#184456

Purpose

Nuclear membrane-targeted iRFP702 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

iRFP702-LaminB1

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTlpA-Wasabi

Plasmid

#85455

Purpose

mWasabi reporter gene gated by wild type TlpA

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A

Promoter

TlpA and TlpA, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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TOP-H2B-mCherry-DD

Plasmid

#96894

Purpose

Reporter to monitor translation of 5'TOP mRNA

Depositor

Tobias Meyer

Article

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

TOP-H2B-mCherry-DD

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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Human NCKX6-FlagTag (pcDNA3.1+)

Plasmid

#75217

Purpose

Mammalian expression of SLC8B1

Depositor

Jonathan Lytton

Article

Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

Insert

SLC8B1 (SLC8B1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pVITRO1-BFP-CAAX

Plasmid

#184459

Purpose

Plasma membrane-targeted EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

EBFP2-CAAX

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pWN172

Plasmid

#215291

Purpose

Expresses sfGFP under the Anderson J23113 promoter with an RSF1010 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Tags

His

Expression

Bacterial

Promoter

J23113

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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